Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo
The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia le...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/8027537 |
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| author | Chanyong Yun Youngchul Jung Wonjoo Chun Beodeul Yang Junghyun Ryu Chiyeon Lim Jung-Hoon Kim Hyungwoo Kim Su-In Cho |
| author_facet | Chanyong Yun Youngchul Jung Wonjoo Chun Beodeul Yang Junghyun Ryu Chiyeon Lim Jung-Hoon Kim Hyungwoo Kim Su-In Cho |
| author_sort | Chanyong Yun |
| collection | DOAJ |
| description | The leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia leaf extract (ALE) using CD mice and Raw 264.7 cells. The effects of ALE on histopathological changes and cytokine production in ear tissues were assessed in mice with CD induced by 1-fluoro-2,4-dinitrobenzene (DNFB). Moreover, the anti-inflammatory effects on production levels of prostaglandin E2 (PGE2) and nitric oxide (NO) and expression levels of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) were investigated in Raw 264.7 cells. Topical application of ALE effectively prevented ear swelling induced by repeated DNFB application. ALE prevented epidermal hyperplasia and infiltration of immune cells and lowered the production of interferon- (IFN-) gamma (γ), tumour necrosis factor- (TNF-) alpha (α), and interleukin- (IL-) 6 in inflamed tissues. In addition, ALE inhibited expression of COX-2 and iNOS and production of NO and PGE2 in Raw 264.7 cells. These results indicate that Artemisia leaf can be used as a therapeutic agent for inflammatory skin diseases and that its anti-inflammatory effects are closely related to the inhibition of inflammatory mediator release from macrophages and inflammatory cytokine production in inflamed tissues. |
| format | Article |
| id | doaj-art-0d821651585c40d288e04216855610c8 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0d821651585c40d288e04216855610c82025-02-03T01:09:00ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/80275378027537Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In VivoChanyong Yun0Youngchul Jung1Wonjoo Chun2Beodeul Yang3Junghyun Ryu4Chiyeon Lim5Jung-Hoon Kim6Hyungwoo Kim7Su-In Cho8Division of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaCollege of Medicine, Dongguk University, Goyang-si, Gyeonggi-do 10326, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaDivision of Pharmacology, School of Korean Medicine, Pusan National University, Busan, Republic of KoreaThe leaves of Artemisia argyi Lev. et Vant. and A. princeps Pamp. are well known medicinal herbs used to treat patients in China, Japan, and Korea with skin problems such as eczema and itching, as well as abdominal pain and dysmenorrhoea. We investigated the anti-inflammatory effects of Artemisia leaf extract (ALE) using CD mice and Raw 264.7 cells. The effects of ALE on histopathological changes and cytokine production in ear tissues were assessed in mice with CD induced by 1-fluoro-2,4-dinitrobenzene (DNFB). Moreover, the anti-inflammatory effects on production levels of prostaglandin E2 (PGE2) and nitric oxide (NO) and expression levels of cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) were investigated in Raw 264.7 cells. Topical application of ALE effectively prevented ear swelling induced by repeated DNFB application. ALE prevented epidermal hyperplasia and infiltration of immune cells and lowered the production of interferon- (IFN-) gamma (γ), tumour necrosis factor- (TNF-) alpha (α), and interleukin- (IL-) 6 in inflamed tissues. In addition, ALE inhibited expression of COX-2 and iNOS and production of NO and PGE2 in Raw 264.7 cells. These results indicate that Artemisia leaf can be used as a therapeutic agent for inflammatory skin diseases and that its anti-inflammatory effects are closely related to the inhibition of inflammatory mediator release from macrophages and inflammatory cytokine production in inflamed tissues.http://dx.doi.org/10.1155/2016/8027537 |
| spellingShingle | Chanyong Yun Youngchul Jung Wonjoo Chun Beodeul Yang Junghyun Ryu Chiyeon Lim Jung-Hoon Kim Hyungwoo Kim Su-In Cho Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo Mediators of Inflammation |
| title | Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo |
| title_full | Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo |
| title_fullStr | Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo |
| title_full_unstemmed | Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo |
| title_short | Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo |
| title_sort | anti inflammatory effects of artemisia leaf extract in mice with contact dermatitis in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2016/8027537 |
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