Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks
The ovary is the main controller of female fertility, unfortunately, its onset of aging processes was earlier than other organs. Our previous studies showed calcium (Ca) deficiency reduced ovarian weight and declined numbers of dominant follicles in an avian model. However, whether Ca provided a fun...
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124011295 |
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| author | Cheng-long Jin Sheng-lin Wang Shuang Wang Ya-nan Zhang Wei-guang Xia Chang Zhang Xue-bing Huang Kai-chao Li Chun-tian Zheng Wei Chen |
| author_facet | Cheng-long Jin Sheng-lin Wang Shuang Wang Ya-nan Zhang Wei-guang Xia Chang Zhang Xue-bing Huang Kai-chao Li Chun-tian Zheng Wei Chen |
| author_sort | Cheng-long Jin |
| collection | DOAJ |
| description | The ovary is the main controller of female fertility, unfortunately, its onset of aging processes was earlier than other organs. Our previous studies showed calcium (Ca) deficiency reduced ovarian weight and declined numbers of dominant follicles in an avian model. However, whether Ca provided a functional role in follicle development of aged avian, and its further mechanism was still unknown. In this study, fifty180-day-old and fifty 700-day-old female Longyan ducks were divided into the young group and the aged group to illustrate the differences of Ca signaling and further mechanisms. We found the poor productive performance of aged ducks was correlated with follicle decreased numbers and atrophied microstructure, and restricted antioxidant ability of granulosa cells (GCs). Then, according to RNA-Seq analysis, we detected those aged ducks displayed lower Ca concentration in the ovary, while Ca channel related gene expression was increased in GCs to maintain homeostasis. Moreover, the cyclic adenosine monophosphate (cAMP) concentration and cAMP synthase family related genes were also decreased in GCs of aged ducks. Fortunately, medium supplemented with Ca channel-activator A23187 enhanced GC viability, antioxidant ability, tight junction ability, and increased cAMP concentration by improved cAMP metabolism, otherwise, the opposite changes were observed with Ca2+-chelating agent EGTA or Ca channel-inhibitor Verapamil supplementation. In conclusion, aging disordered Ca signaling to limit cAMP metabolism, then decreased antioxidant ability and inhibited proliferation and migration ability of GCs. Thus, the follicle development and reproductive performance were restricted in aged avian. |
| format | Article |
| id | doaj-art-0d736d1043da4a39beb33761aa8264b3 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-0d736d1043da4a39beb33761aa8264b32025-01-22T05:40:26ZengElsevierPoultry Science0032-57912025-01-011041104551Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducksCheng-long Jin0Sheng-lin Wang1Shuang Wang2Ya-nan Zhang3Wei-guang Xia4Chang Zhang5Xue-bing Huang6Kai-chao Li7Chun-tian Zheng8Wei Chen9State Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaState Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaCorresponding author.; State Key Laboratory of Swine and Poultry Breeding Industry/Institute of Animal Science, Guangdong Academy of Agricultural Sciences/Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs/ Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Guangzhou 510640, PR ChinaThe ovary is the main controller of female fertility, unfortunately, its onset of aging processes was earlier than other organs. Our previous studies showed calcium (Ca) deficiency reduced ovarian weight and declined numbers of dominant follicles in an avian model. However, whether Ca provided a functional role in follicle development of aged avian, and its further mechanism was still unknown. In this study, fifty180-day-old and fifty 700-day-old female Longyan ducks were divided into the young group and the aged group to illustrate the differences of Ca signaling and further mechanisms. We found the poor productive performance of aged ducks was correlated with follicle decreased numbers and atrophied microstructure, and restricted antioxidant ability of granulosa cells (GCs). Then, according to RNA-Seq analysis, we detected those aged ducks displayed lower Ca concentration in the ovary, while Ca channel related gene expression was increased in GCs to maintain homeostasis. Moreover, the cyclic adenosine monophosphate (cAMP) concentration and cAMP synthase family related genes were also decreased in GCs of aged ducks. Fortunately, medium supplemented with Ca channel-activator A23187 enhanced GC viability, antioxidant ability, tight junction ability, and increased cAMP concentration by improved cAMP metabolism, otherwise, the opposite changes were observed with Ca2+-chelating agent EGTA or Ca channel-inhibitor Verapamil supplementation. In conclusion, aging disordered Ca signaling to limit cAMP metabolism, then decreased antioxidant ability and inhibited proliferation and migration ability of GCs. Thus, the follicle development and reproductive performance were restricted in aged avian.http://www.sciencedirect.com/science/article/pii/S0032579124011295AgingCalciumcAMP metabolismAntioxidant abilityFollicle development |
| spellingShingle | Cheng-long Jin Sheng-lin Wang Shuang Wang Ya-nan Zhang Wei-guang Xia Chang Zhang Xue-bing Huang Kai-chao Li Chun-tian Zheng Wei Chen Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks Poultry Science Aging Calcium cAMP metabolism Antioxidant ability Follicle development |
| title | Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks |
| title_full | Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks |
| title_fullStr | Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks |
| title_full_unstemmed | Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks |
| title_short | Age-related calcium signaling disturbance restricted cAMP metabolism and induced ovarian oxidation stress in laying ducks |
| title_sort | age related calcium signaling disturbance restricted camp metabolism and induced ovarian oxidation stress in laying ducks |
| topic | Aging Calcium cAMP metabolism Antioxidant ability Follicle development |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124011295 |
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