Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System
Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were perfo...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2011-09-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1284 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | Liposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently. |
| format | Article |
| id | doaj-art-0d4e545b646e4f92ba07d2b22b00a530 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2011-09-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-0d4e545b646e4f92ba07d2b22b00a5302025-08-20T03:16:29ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862011-09-018310.21123/bsj.8.3.728-735Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay SystemBaghdad Science JournalLiposome-mediated transfection of cancer cells provide a valuable experimental technique to study cellular gene expression and may also be adapted for gene therapy studies. However, the widely recognized advantage of liposome-mediated transfection is high efficiency. Therefore, this study were performed to optimize transfection techniques in human larynx carcinoma cell line Hep-2 using the commercial synthetic lipid TransFast™ Reagent and monitoring the expression efficiency by using the pSV-?-galactosidase Control Vector which encoded ?-galactosidase, maximum transfection efficiency were achieved with TransFast™ Reagent used at the Charge ratios of 2:1 and 0.5 µg DNA/ml, this is indicate that TransFast™ Reagent can be used as an efficient transfection agent to deliver foreign DNA into human larynx carcinoma cell line Hep-2 and expression of the transgene efficiently.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1284Reporter gene, Hep-2, transfection |
| spellingShingle | Baghdad Science Journal Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System مجلة بغداد للعلوم Reporter gene, Hep-2, transfection |
| title | Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System |
| title_full | Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System |
| title_fullStr | Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System |
| title_full_unstemmed | Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System |
| title_short | Evaluation of Transfected HEP-2 Cell Line Using ß-Galactosidase Reporter Assay System |
| title_sort | evaluation of transfected hep 2 cell line using 223 galactosidase reporter assay system |
| topic | Reporter gene, Hep-2, transfection |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1284 |
| work_keys_str_mv | AT baghdadsciencejournal evaluationoftransfectedhep2celllineusing223galactosidasereporterassaysystem |