Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water
The in vitro survival of Escherichia coli O157:H7 at 15∘C under two experimental conditions (sterile soil and sterile natural water) was examined. DNA microarrays of the entire set of E. coli O157:H7 genes were used to measure the genomic expression patterns after 14 days. Although the populations...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2011/340506 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567354626670592 |
|---|---|
| author | Ashley D. Duffitt Robert T. Reber Andrew Whipple Christian Chauret |
| author_facet | Ashley D. Duffitt Robert T. Reber Andrew Whipple Christian Chauret |
| author_sort | Ashley D. Duffitt |
| collection | DOAJ |
| description | The in vitro survival of Escherichia coli O157:H7 at 15∘C
under two experimental conditions (sterile soil and sterile natural water) was examined. DNA microarrays of the entire set of E. coli O157:H7 genes were used to measure the genomic expression patterns after 14 days. Although the populations declined, some E. coli O157:H7 cells survived in sterile stream water up to 234 days and in sterile soil for up to 179 days. Cells incubated in soil microcosms for 14 days expressed genes for antibiotic resistance, biosynthesis, DNA replication and modification, metabolism, phages, transposons, plasmids, pathogenesis and virulence, antibiotic resistance, ribosomal proteins, the stress response, transcription, translation, and transport and binding proteins at significantly higher levels than cells grown in Luria broth. These results suggest that E. coli O157:H7 may develop a different phenotype during transport through the environment. Furthermore, this pathogen may become more resistant to antibiotics making subsequent infections more difficult to treat. |
| format | Article |
| id | doaj-art-0d183d12471e4abd994b00c894fac619 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-0d183d12471e4abd994b00c894fac6192025-02-03T01:01:35ZengWileyInternational Journal of Microbiology1687-918X1687-91982011-01-01201110.1155/2011/340506340506Gene Expression during Survival of Escherichia coli O157:H7 in Soil and WaterAshley D. Duffitt0Robert T. Reber1Andrew Whipple2Christian Chauret3Earth and Environmental Sciences, Taylor University, Upland, IN 46989, USAEarth and Environmental Sciences, Taylor University, Upland, IN 46989, USAEarth and Environmental Sciences, Taylor University, Upland, IN 46989, USADepartment of Natural, Information, and Mathematical Sciences, IN University Kokomo, 2300 South Washington Street, Kokomo, Indiana 46904-9003, USAThe in vitro survival of Escherichia coli O157:H7 at 15∘C under two experimental conditions (sterile soil and sterile natural water) was examined. DNA microarrays of the entire set of E. coli O157:H7 genes were used to measure the genomic expression patterns after 14 days. Although the populations declined, some E. coli O157:H7 cells survived in sterile stream water up to 234 days and in sterile soil for up to 179 days. Cells incubated in soil microcosms for 14 days expressed genes for antibiotic resistance, biosynthesis, DNA replication and modification, metabolism, phages, transposons, plasmids, pathogenesis and virulence, antibiotic resistance, ribosomal proteins, the stress response, transcription, translation, and transport and binding proteins at significantly higher levels than cells grown in Luria broth. These results suggest that E. coli O157:H7 may develop a different phenotype during transport through the environment. Furthermore, this pathogen may become more resistant to antibiotics making subsequent infections more difficult to treat.http://dx.doi.org/10.1155/2011/340506 |
| spellingShingle | Ashley D. Duffitt Robert T. Reber Andrew Whipple Christian Chauret Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water International Journal of Microbiology |
| title | Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water |
| title_full | Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water |
| title_fullStr | Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water |
| title_full_unstemmed | Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water |
| title_short | Gene Expression during Survival of Escherichia coli O157:H7 in Soil and Water |
| title_sort | gene expression during survival of escherichia coli o157 h7 in soil and water |
| url | http://dx.doi.org/10.1155/2011/340506 |
| work_keys_str_mv | AT ashleydduffitt geneexpressionduringsurvivalofescherichiacolio157h7insoilandwater AT roberttreber geneexpressionduringsurvivalofescherichiacolio157h7insoilandwater AT andrewwhipple geneexpressionduringsurvivalofescherichiacolio157h7insoilandwater AT christianchauret geneexpressionduringsurvivalofescherichiacolio157h7insoilandwater |