Activation of Macrophages by Oligomeric Proteins of Different Size and Origin
Activation of macrophages is one of the key processes in generating the immune response against pathogens or misfolded/aggregated otherwise unharmful host’s proteins. Antigens and their immune complexes (IC) may shape macrophage phenotype in various directions. Data on the impact of protein structur...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2018/7501985 |
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| _version_ | 1832562095863889920 |
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| author | Indrė Dalgėdienė Asta Lučiūnaitė Aurelija Žvirblienė |
| author_facet | Indrė Dalgėdienė Asta Lučiūnaitė Aurelija Žvirblienė |
| author_sort | Indrė Dalgėdienė |
| collection | DOAJ |
| description | Activation of macrophages is one of the key processes in generating the immune response against pathogens or misfolded/aggregated otherwise unharmful host’s proteins. Antigens and their immune complexes (IC) may shape macrophage phenotype in various directions. Data on the impact of protein structure during inflammation are evident; however, some separate steps of this process involving changes in macrophage phenotype are not fully understood. Our aim was to investigate the phenotype of macrophages after activation with different oligomeric proteins and their IC. We have used amyloid beta (Aβ1–42) that plays a role in neurodegenerative inflammation as a model of host-associated protein and three oligomeric viral antigens as pathogen-associated proteins. Murine cell lines J774, BV-2, and macrophage primary cell culture were treated with oligomeric proteins and their IC. After 48 h, expression of surface markers F4/80, CD68, CD86, and CD206 and secreted cytokines IL-10, IL-12, IL-23, and TNF-α was analysed. Aβ1–42 oligomers stimulated expression of both inflammatory and anti-inflammatory molecules; however, fibrils induced less intense expression of markers investigated as compared to small and large oligomers. Two out of three viral oligomeric proteins induced the inflammatory response of macrophages. Data suggest that macrophage activation pattern depends on the origin, size, and structure of oligomeric proteins. |
| format | Article |
| id | doaj-art-0d14047afce64a1689e937a364f92cd1 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0d14047afce64a1689e937a364f92cd12025-02-03T01:23:25ZengWileyMediators of Inflammation0962-93511466-18612018-01-01201810.1155/2018/75019857501985Activation of Macrophages by Oligomeric Proteins of Different Size and OriginIndrė Dalgėdienė0Asta Lučiūnaitė1Aurelija Žvirblienė2Institute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, LithuaniaInstitute of Biotechnology, Life Sciences Center, Vilnius University, Vilnius LT-10257, LithuaniaActivation of macrophages is one of the key processes in generating the immune response against pathogens or misfolded/aggregated otherwise unharmful host’s proteins. Antigens and their immune complexes (IC) may shape macrophage phenotype in various directions. Data on the impact of protein structure during inflammation are evident; however, some separate steps of this process involving changes in macrophage phenotype are not fully understood. Our aim was to investigate the phenotype of macrophages after activation with different oligomeric proteins and their IC. We have used amyloid beta (Aβ1–42) that plays a role in neurodegenerative inflammation as a model of host-associated protein and three oligomeric viral antigens as pathogen-associated proteins. Murine cell lines J774, BV-2, and macrophage primary cell culture were treated with oligomeric proteins and their IC. After 48 h, expression of surface markers F4/80, CD68, CD86, and CD206 and secreted cytokines IL-10, IL-12, IL-23, and TNF-α was analysed. Aβ1–42 oligomers stimulated expression of both inflammatory and anti-inflammatory molecules; however, fibrils induced less intense expression of markers investigated as compared to small and large oligomers. Two out of three viral oligomeric proteins induced the inflammatory response of macrophages. Data suggest that macrophage activation pattern depends on the origin, size, and structure of oligomeric proteins.http://dx.doi.org/10.1155/2018/7501985 |
| spellingShingle | Indrė Dalgėdienė Asta Lučiūnaitė Aurelija Žvirblienė Activation of Macrophages by Oligomeric Proteins of Different Size and Origin Mediators of Inflammation |
| title | Activation of Macrophages by Oligomeric Proteins of Different Size and Origin |
| title_full | Activation of Macrophages by Oligomeric Proteins of Different Size and Origin |
| title_fullStr | Activation of Macrophages by Oligomeric Proteins of Different Size and Origin |
| title_full_unstemmed | Activation of Macrophages by Oligomeric Proteins of Different Size and Origin |
| title_short | Activation of Macrophages by Oligomeric Proteins of Different Size and Origin |
| title_sort | activation of macrophages by oligomeric proteins of different size and origin |
| url | http://dx.doi.org/10.1155/2018/7501985 |
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