Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia
Ischemic postconditioning (PC) is proved to efficiently protect diabetic patients with acute myocardial infarction from ischemia-reperfusion injury. We aimed to explore the protective roles of ischemic PC on diabetic retinopathy in tree shrews with diabetic cerebral ischemia. A diabetic tree shrew m...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2020/6286571 |
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| author | Ling Zhao Qiwei Liao Yueting Zhang Shufen Tan Shuqing Li Tingyu Ke |
| author_facet | Ling Zhao Qiwei Liao Yueting Zhang Shufen Tan Shuqing Li Tingyu Ke |
| author_sort | Ling Zhao |
| collection | DOAJ |
| description | Ischemic postconditioning (PC) is proved to efficiently protect diabetic patients with acute myocardial infarction from ischemia-reperfusion injury. We aimed to explore the protective roles of ischemic PC on diabetic retinopathy in tree shrews with diabetic cerebral ischemia. A diabetic tree shrew model was established through high-fat diet feeding combined with streptozotocin (STZ) injection, while cortical thrombotic cerebral ischemia was induced photochemically. Tree shrews were divided into the normal control group, sham operation group, diabetes mellitus group, diabetes mellitus+cerebral ischemia group, and diabetes mellitus+cerebral ischemia+PC group (in which the tree shrews with diabetic cerebral ischemia were treated with ischemic PC). H&E staining was used to examine the pathological changes in the retina, and immunohistochemistry was performed to determine the retinal expression of VEGF (vascular endothelial growth factor). The modeling resulted in 77% tree shrews with diabetes. Ischemic PC reduced the blood glucose levels in the tree shrews with diabetic cerebral ischemia. Tree shrews with diabetes had thinned retina with disordered structures, and these pathological changes were aggravated after cerebral ischemia. The retinopathy was alleviated after ischemic PC. Retina expression of VEGF was mainly distributed in the ganglion cell layer in tree shrews. Diabetes and cerebral ischemia increased retinal VEGF expression in a step-wise manner, while additional ischemic PC reduced retinal VEGF expression. Therefore, ischemic PC effectively alleviates retinopathy in tree shrews with diabetic cerebral ischemia, and this effect is associated with reduced retinal VEGF expression. |
| format | Article |
| id | doaj-art-0cdc8ce11d2943b1b5023eea50ad0730 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-0cdc8ce11d2943b1b5023eea50ad07302025-02-03T01:24:39ZengWileyJournal of Diabetes Research2314-67452314-67532020-01-01202010.1155/2020/62865716286571Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral IschemiaLing Zhao0Qiwei Liao1Yueting Zhang2Shufen Tan3Shuqing Li4Tingyu Ke5Department of Endocrinology, The Second Affiliated Hospital of Kunming Medical University, Yunnan 650101, ChinaDepartment of Cardiology, The Yan-an Affiliated Hospital of Kunming Medical University, Yunnan 650051, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Kunming Medical University, Yunnan 650101, ChinaDepartment of Gynecologic Oncology, The Third Affiliated Hospital of Kunming Medical University, Yunnan 650101, ChinaDepartment of Pathophysiology, Kunming Medical University, Yunnan 650050, ChinaDepartment of Endocrinology, The Second Affiliated Hospital of Kunming Medical University, Yunnan 650101, ChinaIschemic postconditioning (PC) is proved to efficiently protect diabetic patients with acute myocardial infarction from ischemia-reperfusion injury. We aimed to explore the protective roles of ischemic PC on diabetic retinopathy in tree shrews with diabetic cerebral ischemia. A diabetic tree shrew model was established through high-fat diet feeding combined with streptozotocin (STZ) injection, while cortical thrombotic cerebral ischemia was induced photochemically. Tree shrews were divided into the normal control group, sham operation group, diabetes mellitus group, diabetes mellitus+cerebral ischemia group, and diabetes mellitus+cerebral ischemia+PC group (in which the tree shrews with diabetic cerebral ischemia were treated with ischemic PC). H&E staining was used to examine the pathological changes in the retina, and immunohistochemistry was performed to determine the retinal expression of VEGF (vascular endothelial growth factor). The modeling resulted in 77% tree shrews with diabetes. Ischemic PC reduced the blood glucose levels in the tree shrews with diabetic cerebral ischemia. Tree shrews with diabetes had thinned retina with disordered structures, and these pathological changes were aggravated after cerebral ischemia. The retinopathy was alleviated after ischemic PC. Retina expression of VEGF was mainly distributed in the ganglion cell layer in tree shrews. Diabetes and cerebral ischemia increased retinal VEGF expression in a step-wise manner, while additional ischemic PC reduced retinal VEGF expression. Therefore, ischemic PC effectively alleviates retinopathy in tree shrews with diabetic cerebral ischemia, and this effect is associated with reduced retinal VEGF expression.http://dx.doi.org/10.1155/2020/6286571 |
| spellingShingle | Ling Zhao Qiwei Liao Yueting Zhang Shufen Tan Shuqing Li Tingyu Ke Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia Journal of Diabetes Research |
| title | Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia |
| title_full | Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia |
| title_fullStr | Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia |
| title_full_unstemmed | Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia |
| title_short | Ischemic Postconditioning Mitigates Retinopathy in Tree Shrews with Diabetic Cerebral Ischemia |
| title_sort | ischemic postconditioning mitigates retinopathy in tree shrews with diabetic cerebral ischemia |
| url | http://dx.doi.org/10.1155/2020/6286571 |
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