The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats
<p><i>Lin28a</i> and <i>Lin28b</i> serve as crucial regulators in gametogenesis. However, it remains unclear whether the two genes are expressed in goat ovarian tissues. In the present study, we examined the distribution of Lin28a proteins in the ovarian tissues of ju...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Copernicus Publications
2025-07-01
|
| Series: | Archives Animal Breeding |
| Online Access: | https://aab.copernicus.org/articles/68/435/2025/aab-68-435-2025.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849429514784866304 |
|---|---|
| author | H. Liang X. Du Y. Xie M. Chu G. Cao |
| author_facet | H. Liang X. Du Y. Xie M. Chu G. Cao |
| author_sort | H. Liang |
| collection | DOAJ |
| description | <p><i>Lin28a</i> and <i>Lin28b</i> serve as crucial regulators in gametogenesis. However, it remains unclear whether the two genes are expressed in goat ovarian tissues. In the present study, we examined the distribution of Lin28a proteins in the ovarian tissues of juvenile and pubertal Jining Grey (JG) goats. Additionally, we investigated the mutations in <i>Lin28a</i> and <i>Lin28b</i> and their association with litter size in JG goats. Lin28a proteins were predominantly expressed in the oocytes and granulosa cells of the follicles at different stages of development. Lin28a was also expressed in the corpus luteum, and its expression level declined as the corpus luteum formed and degenerated into the corpus albicans. A mutation (12298T>C) was identified in intron 2 of <i>Lin28a</i>, and there was no significant difference (<span class="inline-formula"><i>P</i>>0.05</span>) in terms of the kidding number among the three genotypes of JG goats. For <i>Lin28b</i>, a mutation (718A>T) in exon 4 was found, presenting three genotypes. The frequency of genotypes AA, AT, and TT was 0.466, 0.504, and 0.03, respectively. The A allele was the dominant allele in JG goats, with an allele frequency of 0.718. At this locus, JG female goats with genotypes TT and AT had 0.83 (<span class="inline-formula"><i>P</i><0.01</span>) and 0.48 (<span class="inline-formula"><i>P</i><0.05</span>) more kids than the goats with genotype AA, and no significant difference (<span class="inline-formula"><i>P</i>>0.05</span>) was observed in terms of litter size between the TT and AT genotypes. The present study preliminarily indicated an association between the T allele at the 718 locus in <i>Lin28b</i> and a larger litter size in JG goats. Moreover, Lin28a was widely expressed in follicles. These results suggest a potential DNA marker for improving the kidding number in goats and hold significance for goat breeding.</p> |
| format | Article |
| id | doaj-art-0c4826d75b4248a09352133b2161eb82 |
| institution | Kabale University |
| issn | 0003-9438 2363-9822 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Copernicus Publications |
| record_format | Article |
| series | Archives Animal Breeding |
| spelling | doaj-art-0c4826d75b4248a09352133b2161eb822025-08-20T03:28:21ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222025-07-016843544310.5194/aab-68-435-2025The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goatsH. Liang0X. Du1Y. Xie2M. Chu3G. Cao4School of Agriculture and Biology, Liaocheng University, Liaocheng 252059, ChinaSchool of Agriculture and Biology, Liaocheng University, Liaocheng 252059, ChinaSchool of Agriculture and Biology, Liaocheng University, Liaocheng 252059, ChinaState Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaSchool of Agriculture and Biology, Liaocheng University, Liaocheng 252059, China<p><i>Lin28a</i> and <i>Lin28b</i> serve as crucial regulators in gametogenesis. However, it remains unclear whether the two genes are expressed in goat ovarian tissues. In the present study, we examined the distribution of Lin28a proteins in the ovarian tissues of juvenile and pubertal Jining Grey (JG) goats. Additionally, we investigated the mutations in <i>Lin28a</i> and <i>Lin28b</i> and their association with litter size in JG goats. Lin28a proteins were predominantly expressed in the oocytes and granulosa cells of the follicles at different stages of development. Lin28a was also expressed in the corpus luteum, and its expression level declined as the corpus luteum formed and degenerated into the corpus albicans. A mutation (12298T>C) was identified in intron 2 of <i>Lin28a</i>, and there was no significant difference (<span class="inline-formula"><i>P</i>>0.05</span>) in terms of the kidding number among the three genotypes of JG goats. For <i>Lin28b</i>, a mutation (718A>T) in exon 4 was found, presenting three genotypes. The frequency of genotypes AA, AT, and TT was 0.466, 0.504, and 0.03, respectively. The A allele was the dominant allele in JG goats, with an allele frequency of 0.718. At this locus, JG female goats with genotypes TT and AT had 0.83 (<span class="inline-formula"><i>P</i><0.01</span>) and 0.48 (<span class="inline-formula"><i>P</i><0.05</span>) more kids than the goats with genotype AA, and no significant difference (<span class="inline-formula"><i>P</i>>0.05</span>) was observed in terms of litter size between the TT and AT genotypes. The present study preliminarily indicated an association between the T allele at the 718 locus in <i>Lin28b</i> and a larger litter size in JG goats. Moreover, Lin28a was widely expressed in follicles. These results suggest a potential DNA marker for improving the kidding number in goats and hold significance for goat breeding.</p>https://aab.copernicus.org/articles/68/435/2025/aab-68-435-2025.pdf |
| spellingShingle | H. Liang X. Du Y. Xie M. Chu G. Cao The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats Archives Animal Breeding |
| title | The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats |
| title_full | The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats |
| title_fullStr | The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats |
| title_full_unstemmed | The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats |
| title_short | The expression of Lin28a in the ovaries and the association of <i>Lin28a</i> and <i>Lin28b</i> with litter size in goats |
| title_sort | expression of lin28a in the ovaries and the association of i lin28a i and i lin28b i with litter size in goats |
| url | https://aab.copernicus.org/articles/68/435/2025/aab-68-435-2025.pdf |
| work_keys_str_mv | AT hliang theexpressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT xdu theexpressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT yxie theexpressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT mchu theexpressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT gcao theexpressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT hliang expressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT xdu expressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT yxie expressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT mchu expressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats AT gcao expressionoflin28aintheovariesandtheassociationofilin28aiandilin28biwithlittersizeingoats |