Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota
This research aimed to investigate the antidiabetic activity, underlying mechanisms, and gut microbiota regulation of aloin. The insulin-resistant HepG2 (IR-HepG2) cell model and the type 2 diabetic (T2D) mouse model were successfully established using dexamethasone and a high-fat high-sucrose diet...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2022-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000859 |
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| author | Ruting Zhong Lanbin Chen Yuanyuan Liu Shouxia Xie Sumei Li Bin Liu Chao Zhao |
| author_facet | Ruting Zhong Lanbin Chen Yuanyuan Liu Shouxia Xie Sumei Li Bin Liu Chao Zhao |
| author_sort | Ruting Zhong |
| collection | DOAJ |
| description | This research aimed to investigate the antidiabetic activity, underlying mechanisms, and gut microbiota regulation of aloin. The insulin-resistant HepG2 (IR-HepG2) cell model and the type 2 diabetic (T2D) mouse model were successfully established using dexamethasone and a high-fat high-sucrose diet with low-dose streptozotocin, respectively. Aloin intervention increased glucose consumption and stimulated the activity of hexokinase and pyruvate dehydrogenase in IR-HepG2 cells. Additionally, it diminished the weight loss, reduced fasting blood glucose levels and hemoglobin A1c activity, and promoted glucose tolerance and fasting serum insulin activity in T2D mice. Histopathological analysis of the liver indicated hepatic protection by aloin. Additionally, aloin treatment inhibited the protein expression of c-Jun N-terminal kinases and activated that of IRS1/PI3K/Akt in the liver. Moreover, aloin modulated the bacterial community in the gut by raising the abundance of Bacteroidota and reducing the richness of Firmicutes, Proteobacteria, and Actinobacteriota. Thus, aloin ameliorated IR via activating IRS1/PI3K/Akt signaling pathway and regulating the gut microbiota, and it may be promising candidate as functional food for diabetic therapy. |
| format | Article |
| id | doaj-art-0c31fbf835bf4073a9814ea4a93f728a |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-0c31fbf835bf4073a9814ea4a93f728a2025-02-03T00:27:31ZengTsinghua University PressFood Science and Human Wellness2213-45302022-01-01111189198Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiotaRuting Zhong0Lanbin Chen1Yuanyuan Liu2Shouxia Xie3Sumei Li4Bin Liu5Chao Zhao6College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaDepartment of Pharmacology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, ChinaDepartment of Pharmacology, Shenzhen People's Hospital (The Second Clinical Medical College, Jinan University; The First Affiliated Hospital, Southern University of Science and Technology), Shenzhen 518020, Guangdong, China; Corresponding authors.College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition, Ministry of Education, Fuzhou 350002, China; Corresponding authors.This research aimed to investigate the antidiabetic activity, underlying mechanisms, and gut microbiota regulation of aloin. The insulin-resistant HepG2 (IR-HepG2) cell model and the type 2 diabetic (T2D) mouse model were successfully established using dexamethasone and a high-fat high-sucrose diet with low-dose streptozotocin, respectively. Aloin intervention increased glucose consumption and stimulated the activity of hexokinase and pyruvate dehydrogenase in IR-HepG2 cells. Additionally, it diminished the weight loss, reduced fasting blood glucose levels and hemoglobin A1c activity, and promoted glucose tolerance and fasting serum insulin activity in T2D mice. Histopathological analysis of the liver indicated hepatic protection by aloin. Additionally, aloin treatment inhibited the protein expression of c-Jun N-terminal kinases and activated that of IRS1/PI3K/Akt in the liver. Moreover, aloin modulated the bacterial community in the gut by raising the abundance of Bacteroidota and reducing the richness of Firmicutes, Proteobacteria, and Actinobacteriota. Thus, aloin ameliorated IR via activating IRS1/PI3K/Akt signaling pathway and regulating the gut microbiota, and it may be promising candidate as functional food for diabetic therapy.http://www.sciencedirect.com/science/article/pii/S2213453021000859AloinAntidiabeticGut microbiotaInsulin signaling pathway |
| spellingShingle | Ruting Zhong Lanbin Chen Yuanyuan Liu Shouxia Xie Sumei Li Bin Liu Chao Zhao Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota Food Science and Human Wellness Aloin Antidiabetic Gut microbiota Insulin signaling pathway |
| title | Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota |
| title_full | Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota |
| title_fullStr | Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota |
| title_full_unstemmed | Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota |
| title_short | Anti-diabetic effect of aloin via JNK-IRS1/PI3K pathways and regulation of gut microbiota |
| title_sort | anti diabetic effect of aloin via jnk irs1 pi3k pathways and regulation of gut microbiota |
| topic | Aloin Antidiabetic Gut microbiota Insulin signaling pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000859 |
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