Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue
Background: Diabetes mellitus (DM), one of the most pervasive and enduring metabolic diseases, has been demonstratedto adversely impact male fertility. Conversely, both exercise training and Chrysin have been identified aspotential interventions capable of mitigating the deleterious effects of diabe...
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Royan Institute (ACECR), Tehran
2025-01-01
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| Series: | International Journal of Fertility and Sterility |
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| Online Access: | https://www.ijfs.ir/article_713721_6b3b9dcc78010956e01290e9e049e8e2.pdf |
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| author | Shiva Cheraghi Abajlou Asghar Tofighi Javad Tolouei Azar Amir Afshin Khaki Mazdak Razi |
| author_facet | Shiva Cheraghi Abajlou Asghar Tofighi Javad Tolouei Azar Amir Afshin Khaki Mazdak Razi |
| author_sort | Shiva Cheraghi Abajlou |
| collection | DOAJ |
| description | Background: Diabetes mellitus (DM), one of the most pervasive and enduring metabolic diseases, has been demonstratedto adversely impact male fertility. Conversely, both exercise training and Chrysin have been identified aspotential interventions capable of mitigating the deleterious effects of diabetes on spermatogenesis. Thus, the currentstudy aims to explore the individual and combined influences of Chrysin supplementation and running exerciseon oxidative stress and germ cell apoptosis in the testicular tissue of diabetic adult rats.Materials and Methods: In this experimental study, the DM was induced by streptozotocin (STZ,50 mg/kg). Ratswere divided into control (received STZ solvent), DM-sole, Chrysin-sole (50 mg/kg, daily), moderate-intensityrunning exercise training (MIRET-sole, warm-up, 5 minutes at 30% of Smax1 (Maximum speed); Moderate intensityexercise, 60 minutes at 60% of Smax1, and recovery, 5 minutes to 30% of Smax1), DM+Chrysin, DM+MIRET,and DM+MIRET+Chrysin. Following 8 weeks, the histopathological changes (Johnson’s score, epithelial height,and tubular diameter), testicular malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase(GPX), and the mRNA levels of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax was analyzed.Results: Chrysin solely and simultaneous with MIRET could remarkably (P=0.001) improve the DM-induced histopathologicaldamages, increase the testicular SOD and GPx levels, and decline the DM-increased MDA content.Moreover, our results showed that Chrysin solely and more simultaneously with MIRET could significantly (P=0.001)decrease the mRNA expression of Bax and improve the Bcl-2 expression and rebalance the Bax/Bcl-2 balance.Conclusion: Our findings showed that co-administration of Chrysin along with MIRET can significantly amelioratethe DM-induced histopathological, and biochemical impairments and reduce the pro-apoptotic impact of DMon testicular tissue. |
| format | Article |
| id | doaj-art-0c30796bcd7643159eee10a4658dbb11 |
| institution | Kabale University |
| issn | 2008-076X 2008-0778 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
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| series | International Journal of Fertility and Sterility |
| spelling | doaj-art-0c30796bcd7643159eee10a4658dbb112025-01-18T09:23:38ZengRoyan Institute (ACECR), TehranInternational Journal of Fertility and Sterility2008-076X2008-07782025-01-01191889510.22074/ijfs.2024.2019906.1606713721Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular TissueShiva Cheraghi Abajlou0Asghar Tofighi1Javad Tolouei Azar2Amir Afshin Khaki3Mazdak Razi4Department of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, IranDepartment of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, IranDepartment of Exercise Physiology and Corrective Exercises, Faculty of Sport Sciences, Urmia University, Urmia, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Anatomical Sciences, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, IranBackground: Diabetes mellitus (DM), one of the most pervasive and enduring metabolic diseases, has been demonstratedto adversely impact male fertility. Conversely, both exercise training and Chrysin have been identified aspotential interventions capable of mitigating the deleterious effects of diabetes on spermatogenesis. Thus, the currentstudy aims to explore the individual and combined influences of Chrysin supplementation and running exerciseon oxidative stress and germ cell apoptosis in the testicular tissue of diabetic adult rats.Materials and Methods: In this experimental study, the DM was induced by streptozotocin (STZ,50 mg/kg). Ratswere divided into control (received STZ solvent), DM-sole, Chrysin-sole (50 mg/kg, daily), moderate-intensityrunning exercise training (MIRET-sole, warm-up, 5 minutes at 30% of Smax1 (Maximum speed); Moderate intensityexercise, 60 minutes at 60% of Smax1, and recovery, 5 minutes to 30% of Smax1), DM+Chrysin, DM+MIRET,and DM+MIRET+Chrysin. Following 8 weeks, the histopathological changes (Johnson’s score, epithelial height,and tubular diameter), testicular malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase(GPX), and the mRNA levels of anti-apoptotic gene Bcl-2 and pro-apoptotic gene Bax was analyzed.Results: Chrysin solely and simultaneous with MIRET could remarkably (P=0.001) improve the DM-induced histopathologicaldamages, increase the testicular SOD and GPx levels, and decline the DM-increased MDA content.Moreover, our results showed that Chrysin solely and more simultaneously with MIRET could significantly (P=0.001)decrease the mRNA expression of Bax and improve the Bcl-2 expression and rebalance the Bax/Bcl-2 balance.Conclusion: Our findings showed that co-administration of Chrysin along with MIRET can significantly amelioratethe DM-induced histopathological, and biochemical impairments and reduce the pro-apoptotic impact of DMon testicular tissue.https://www.ijfs.ir/article_713721_6b3b9dcc78010956e01290e9e049e8e2.pdfapoptosischrysindiabetes mellitusexercise trainingtestis |
| spellingShingle | Shiva Cheraghi Abajlou Asghar Tofighi Javad Tolouei Azar Amir Afshin Khaki Mazdak Razi Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue International Journal of Fertility and Sterility apoptosis chrysin diabetes mellitus exercise training testis |
| title | Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue |
| title_full | Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue |
| title_fullStr | Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue |
| title_full_unstemmed | Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue |
| title_short | Combined Effects of Chrysin Supplementation and Exercise Training on Diabetes-Induced Oxidative Stress and Apoptosis in Rat Testicular Tissue |
| title_sort | combined effects of chrysin supplementation and exercise training on diabetes induced oxidative stress and apoptosis in rat testicular tissue |
| topic | apoptosis chrysin diabetes mellitus exercise training testis |
| url | https://www.ijfs.ir/article_713721_6b3b9dcc78010956e01290e9e049e8e2.pdf |
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