Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil
Bdellovibrio spp. are predatory bacteria with great potential as antimicrobial agents. Studies have shown that members of the genus Bdellovibrio exhibit peculiar characteristics that influence their ecological adaptations. In this study, whole genomes of two different Bdellovibrio spp. designated SK...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2018/9402073 |
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| author | Omotayo Opemipo Oyedara Aldo Segura-Cabrera Xianwu Guo Temidayo Oluyomi Elufisan Rafael Alejandro Cantú González Mario A. Rodríguez Pérez |
| author_facet | Omotayo Opemipo Oyedara Aldo Segura-Cabrera Xianwu Guo Temidayo Oluyomi Elufisan Rafael Alejandro Cantú González Mario A. Rodríguez Pérez |
| author_sort | Omotayo Opemipo Oyedara |
| collection | DOAJ |
| description | Bdellovibrio spp. are predatory bacteria with great potential as antimicrobial agents. Studies have shown that members of the genus Bdellovibrio exhibit peculiar characteristics that influence their ecological adaptations. In this study, whole genomes of two different Bdellovibrio spp. designated SKB1291214 and SSB218315 isolated from soil were sequenced. The core genes shared by all the Bdellovibrio spp. considered for the pangenome analysis including the epibiotic B. exovorus were 795. The number of unique genes identified in Bdellovibrio spp. SKB1291214, SSB218315, W, and B. exovorus JJS was 1343, 113, 857, and 1572, respectively. These unique genes encode hydrolytic, chemotaxis, and transporter proteins which might be useful for predation in the Bdellovibrio strains. Furthermore, the two Bdellovibrio strains exhibited differences based on the % GC content, amino acid identity, and 16S rRNA gene sequence. The 16S rRNA gene sequence of Bdellovibrio sp. SKB1291214 shared 99% identity with that of an uncultured Bdellovibrio sp. clone 12L 106 (a pairwise distance of 0.008) and 95–97% identity (a pairwise distance of 0.043) with that of other culturable terrestrial Bdellovibrio spp., including strain SSB218315. In Bdellovibrio sp. SKB1291214, 174 bp sequence was inserted at the host interaction (hit) locus region usually attributed to prey attachment, invasion, and development of host independent Bdellovibrio phenotypes. Also, a gene equivalent to Bd0108 in B. bacteriovorus HD100 was not conserved in Bdellovibrio sp. SKB1291214. The results of this study provided information on the genetic characteristics and diversity of the genus Bdellovibrio that can contribute to their successful applications as a biocontrol agent. |
| format | Article |
| id | doaj-art-0c080b2c3d874030981af31146159a15 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | International Journal of Genomics |
| spelling | doaj-art-0c080b2c3d874030981af31146159a152025-02-03T01:07:28ZengWileyInternational Journal of Genomics2314-436X2314-43782018-01-01201810.1155/2018/94020739402073Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from SoilOmotayo Opemipo Oyedara0Aldo Segura-Cabrera1Xianwu Guo2Temidayo Oluyomi Elufisan3Rafael Alejandro Cantú González4Mario A. Rodríguez Pérez5Instituto Politécnico Nacional, Centro de Biotecnología Genómica, 88710 Reynosa, TAMPS, MexicoRed de Estudios Moleculares Avanzados, Instituto de Ecología, A.C., Xalapa Enriquez, VER, MexicoInstituto Politécnico Nacional, Centro de Biotecnología Genómica, 88710 Reynosa, TAMPS, MexicoInstituto Politécnico Nacional, Centro de Biotecnología Genómica, 88710 Reynosa, TAMPS, MexicoInstituto Politécnico Nacional, Centro de Biotecnología Genómica, 88710 Reynosa, TAMPS, MexicoInstituto Politécnico Nacional, Centro de Biotecnología Genómica, 88710 Reynosa, TAMPS, MexicoBdellovibrio spp. are predatory bacteria with great potential as antimicrobial agents. Studies have shown that members of the genus Bdellovibrio exhibit peculiar characteristics that influence their ecological adaptations. In this study, whole genomes of two different Bdellovibrio spp. designated SKB1291214 and SSB218315 isolated from soil were sequenced. The core genes shared by all the Bdellovibrio spp. considered for the pangenome analysis including the epibiotic B. exovorus were 795. The number of unique genes identified in Bdellovibrio spp. SKB1291214, SSB218315, W, and B. exovorus JJS was 1343, 113, 857, and 1572, respectively. These unique genes encode hydrolytic, chemotaxis, and transporter proteins which might be useful for predation in the Bdellovibrio strains. Furthermore, the two Bdellovibrio strains exhibited differences based on the % GC content, amino acid identity, and 16S rRNA gene sequence. The 16S rRNA gene sequence of Bdellovibrio sp. SKB1291214 shared 99% identity with that of an uncultured Bdellovibrio sp. clone 12L 106 (a pairwise distance of 0.008) and 95–97% identity (a pairwise distance of 0.043) with that of other culturable terrestrial Bdellovibrio spp., including strain SSB218315. In Bdellovibrio sp. SKB1291214, 174 bp sequence was inserted at the host interaction (hit) locus region usually attributed to prey attachment, invasion, and development of host independent Bdellovibrio phenotypes. Also, a gene equivalent to Bd0108 in B. bacteriovorus HD100 was not conserved in Bdellovibrio sp. SKB1291214. The results of this study provided information on the genetic characteristics and diversity of the genus Bdellovibrio that can contribute to their successful applications as a biocontrol agent.http://dx.doi.org/10.1155/2018/9402073 |
| spellingShingle | Omotayo Opemipo Oyedara Aldo Segura-Cabrera Xianwu Guo Temidayo Oluyomi Elufisan Rafael Alejandro Cantú González Mario A. Rodríguez Pérez Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil International Journal of Genomics |
| title | Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil |
| title_full | Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil |
| title_fullStr | Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil |
| title_full_unstemmed | Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil |
| title_short | Whole-Genome Sequencing and Comparative Genome Analysis Provided Insight into the Predatory Features and Genetic Diversity of Two Bdellovibrio Species Isolated from Soil |
| title_sort | whole genome sequencing and comparative genome analysis provided insight into the predatory features and genetic diversity of two bdellovibrio species isolated from soil |
| url | http://dx.doi.org/10.1155/2018/9402073 |
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