Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells
Rab26 GTPase modulates the trafficking of cell surface receptors, such as G protein-coupled receptors including α2-adrenergic receptors in some cell types. However, the effect of Rab26 on β2-adrenergic receptor (β2-AR) trafficking or/and Toll-like receptor 4 (TLR4) expression in human pulmonary micr...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/7538071 |
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| author | Huaping Chen Ming Yuan Chunji Huang Zhi Xu Mingchun Li Chun Zhang Zhan Gao Mingzhou Zhang Jiancheng Xu Hang Qian Jiegen You Binfeng He Guansong Wang Mingdong Hu |
| author_facet | Huaping Chen Ming Yuan Chunji Huang Zhi Xu Mingchun Li Chun Zhang Zhan Gao Mingzhou Zhang Jiancheng Xu Hang Qian Jiegen You Binfeng He Guansong Wang Mingdong Hu |
| author_sort | Huaping Chen |
| collection | DOAJ |
| description | Rab26 GTPase modulates the trafficking of cell surface receptors, such as G protein-coupled receptors including α2-adrenergic receptors in some cell types. However, the effect of Rab26 on β2-adrenergic receptor (β2-AR) trafficking or/and Toll-like receptor 4 (TLR4) expression in human pulmonary microvascular endothelial cells (HPMECs) is still unclear. Here, we investigated the role of Rab26 in regulating the expression of β2-ARs and TLR4 in HPMECs and the effect of these receptors’ imbalance on endothelial cell barrier function. The results showed that there was unbalance expression in these receptors, where β2-AR expression was remarkably reduced, and TLR4 was increased on the cell membrane after lipopolysaccharide (LPS) treatment. Furthermore, we found that Rab26 overexpression not only upregulated β2-ARs but also downregulated TLR4 expression on the cell membrane. Subsequently, the TLR4-related inflammatory response was greatly attenuated, and the hyperpermeability of HPMECs also was partially relived. Taken together, these data suggest that basal Rab26 maintains the balance between β2-ARs and TLR4 on the cell surface, and it might be a potential therapeutic target for diseases involving endothelial barrier dysfunction. |
| format | Article |
| id | doaj-art-0bff7f7cf7a24b99a6a1b1d93d0f27d5 |
| institution | OA Journals |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0bff7f7cf7a24b99a6a1b1d93d0f27d52025-08-20T02:02:55ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/75380717538071Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial CellsHuaping Chen0Ming Yuan1Chunji Huang2Zhi Xu3Mingchun Li4Chun Zhang5Zhan Gao6Mingzhou Zhang7Jiancheng Xu8Hang Qian9Jiegen You10Binfeng He11Guansong Wang12Mingdong Hu13Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaBasic Medical College, Third Military Medical University, Chongqing 400038, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaJiangxi Academy of Medical Sciences, Nanchang City, Jiangxi Province 330006, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaInstitute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, ChinaRab26 GTPase modulates the trafficking of cell surface receptors, such as G protein-coupled receptors including α2-adrenergic receptors in some cell types. However, the effect of Rab26 on β2-adrenergic receptor (β2-AR) trafficking or/and Toll-like receptor 4 (TLR4) expression in human pulmonary microvascular endothelial cells (HPMECs) is still unclear. Here, we investigated the role of Rab26 in regulating the expression of β2-ARs and TLR4 in HPMECs and the effect of these receptors’ imbalance on endothelial cell barrier function. The results showed that there was unbalance expression in these receptors, where β2-AR expression was remarkably reduced, and TLR4 was increased on the cell membrane after lipopolysaccharide (LPS) treatment. Furthermore, we found that Rab26 overexpression not only upregulated β2-ARs but also downregulated TLR4 expression on the cell membrane. Subsequently, the TLR4-related inflammatory response was greatly attenuated, and the hyperpermeability of HPMECs also was partially relived. Taken together, these data suggest that basal Rab26 maintains the balance between β2-ARs and TLR4 on the cell surface, and it might be a potential therapeutic target for diseases involving endothelial barrier dysfunction.http://dx.doi.org/10.1155/2019/7538071 |
| spellingShingle | Huaping Chen Ming Yuan Chunji Huang Zhi Xu Mingchun Li Chun Zhang Zhan Gao Mingzhou Zhang Jiancheng Xu Hang Qian Jiegen You Binfeng He Guansong Wang Mingdong Hu Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells Mediators of Inflammation |
| title | Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells |
| title_full | Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells |
| title_fullStr | Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells |
| title_full_unstemmed | Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells |
| title_short | Endothelial Cell Inflammation and Barriers Are Regulated by the Rab26-Mediated Balance between β2-AR and TLR4 in Pulmonary Microvessel Endothelial Cells |
| title_sort | endothelial cell inflammation and barriers are regulated by the rab26 mediated balance between β2 ar and tlr4 in pulmonary microvessel endothelial cells |
| url | http://dx.doi.org/10.1155/2019/7538071 |
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