Identification of potential substrates of LNX1 in chicken cells
Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase,...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579124012112 |
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| author | Yuhong Liu Qianmei Gao Qi Zhang Chen Li Sha Liu Meng Su Danli Song Guiping Zhao Qinghe Li |
| author_facet | Yuhong Liu Qianmei Gao Qi Zhang Chen Li Sha Liu Meng Su Danli Song Guiping Zhao Qinghe Li |
| author_sort | Yuhong Liu |
| collection | DOAJ |
| description | Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase, catalyzes the ubiquitination and subsequent degradation of specific substrate proteins. A cell line with stable knockdown of the LNX1 gene was developed using chicken macrophages (HD11) as the model. The relative quantitative values of each protein were analyzed using 4D-FastDIA quantitative proteomics. This identified 319 proteins with up-regulated expression, a majority of which relate to the immune system. Overall, this study uncovered potential substrate proteins targeted by LNX1 and their associated biological pathways. The findings suggest that LNX1 could be implicated in the regulation of the chicken innate immune system by mediating protein ubiquitination. |
| format | Article |
| id | doaj-art-0bcca6783ce34d118fa8c27d5dbe3165 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-0bcca6783ce34d118fa8c27d5dbe31652025-01-22T05:40:46ZengElsevierPoultry Science0032-57912025-01-011041104633Identification of potential substrates of LNX1 in chicken cellsYuhong Liu0Qianmei Gao1Qi Zhang2Chen Li3Sha Liu4Meng Su5Danli Song6Guiping Zhao7Qinghe Li8Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaInstitute of Animal Science & Veterinary, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR ChinaKey Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs; State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China; Corresponding author.Ubiquitination of proteins is involved in numerous life activities. In poultry, several ubiquitin ligases tightly control the innate immune response, imbalance of which may result in autoimmune diseases and seriously impair poultry growth, development, and productivity. LNX1, an E3 ubiquitin ligase, catalyzes the ubiquitination and subsequent degradation of specific substrate proteins. A cell line with stable knockdown of the LNX1 gene was developed using chicken macrophages (HD11) as the model. The relative quantitative values of each protein were analyzed using 4D-FastDIA quantitative proteomics. This identified 319 proteins with up-regulated expression, a majority of which relate to the immune system. Overall, this study uncovered potential substrate proteins targeted by LNX1 and their associated biological pathways. The findings suggest that LNX1 could be implicated in the regulation of the chicken innate immune system by mediating protein ubiquitination.http://www.sciencedirect.com/science/article/pii/S0032579124012112HD11LNX1UbiquitinationProteomicsInnate Immunity |
| spellingShingle | Yuhong Liu Qianmei Gao Qi Zhang Chen Li Sha Liu Meng Su Danli Song Guiping Zhao Qinghe Li Identification of potential substrates of LNX1 in chicken cells Poultry Science HD11 LNX1 Ubiquitination Proteomics Innate Immunity |
| title | Identification of potential substrates of LNX1 in chicken cells |
| title_full | Identification of potential substrates of LNX1 in chicken cells |
| title_fullStr | Identification of potential substrates of LNX1 in chicken cells |
| title_full_unstemmed | Identification of potential substrates of LNX1 in chicken cells |
| title_short | Identification of potential substrates of LNX1 in chicken cells |
| title_sort | identification of potential substrates of lnx1 in chicken cells |
| topic | HD11 LNX1 Ubiquitination Proteomics Innate Immunity |
| url | http://www.sciencedirect.com/science/article/pii/S0032579124012112 |
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