Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy
Background. The purpose of the present study was to assess the feasibility of using miR-126 in the urine as a biomarker for diabetic nephropathy. Methods. miRNAs were extracted from the urine samples of T2DM patients with diabetic nephropathy (DN; n=92), T2DM without DN (n=86), and 85 healthy volunt...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/393109 |
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| author | Yang Liu Guangqiang Gao Chun Yang Kun Zhou Baozhong Shen Hongyan Liang Xiaofeng Jiang |
| author_facet | Yang Liu Guangqiang Gao Chun Yang Kun Zhou Baozhong Shen Hongyan Liang Xiaofeng Jiang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Background. The purpose of the present study was to assess the feasibility of using miR-126 in the urine as a biomarker for diabetic nephropathy. Methods. miRNAs were extracted from the urine samples of T2DM patients with diabetic nephropathy (DN; n=92), T2DM without DN (n=86), and 85 healthy volunteers using quantitative reverse transcriptase polymerase chain reaction (real-time polymerase chain reaction) analysis. Stability of urinary miR-126 and factors that affected the stability were assessed. A subgroup analysis was also carried out to compare the urinary miR-126 level in T2DM patients well controlled by the treatment versus those who were not well controlled. Results. Urinary miR-126 was stable when the urine samples were kept at room temperature for extended period of time, 4°C, −20°C, and −80°C for up to 12 hours or subjected to 10 freeze-and-thaw cycle. Urinary miR-126 was significantly higher in T2DM patients with DN (5.76±0.33 versus 3.25±0.45 in T2DM patients without DN). Successful treatment significantly reduced urinary miR-126 in T2DM patients with DN to 3.89±0.52 (P<0.05). Conclusion. miR-126 in the urine is stable and it could be used as a biomarker of DN and to monitor the treatment response. |
| format | Article |
| id | doaj-art-0bb120cab6a6476f87c8fb549e844752 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-0bb120cab6a6476f87c8fb549e8447522025-02-03T01:30:17ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/393109393109Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic NephropathyYang Liu0Guangqiang Gao1Chun Yang2Kun Zhou3Baozhong Shen4Hongyan Liang5Xiaofeng Jiang6Department of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaDepartment of Clinical Biochemistry Laboratory, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, ChinaBackground. The purpose of the present study was to assess the feasibility of using miR-126 in the urine as a biomarker for diabetic nephropathy. Methods. miRNAs were extracted from the urine samples of T2DM patients with diabetic nephropathy (DN; n=92), T2DM without DN (n=86), and 85 healthy volunteers using quantitative reverse transcriptase polymerase chain reaction (real-time polymerase chain reaction) analysis. Stability of urinary miR-126 and factors that affected the stability were assessed. A subgroup analysis was also carried out to compare the urinary miR-126 level in T2DM patients well controlled by the treatment versus those who were not well controlled. Results. Urinary miR-126 was stable when the urine samples were kept at room temperature for extended period of time, 4°C, −20°C, and −80°C for up to 12 hours or subjected to 10 freeze-and-thaw cycle. Urinary miR-126 was significantly higher in T2DM patients with DN (5.76±0.33 versus 3.25±0.45 in T2DM patients without DN). Successful treatment significantly reduced urinary miR-126 in T2DM patients with DN to 3.89±0.52 (P<0.05). Conclusion. miR-126 in the urine is stable and it could be used as a biomarker of DN and to monitor the treatment response.http://dx.doi.org/10.1155/2014/393109 |
| spellingShingle | Yang Liu Guangqiang Gao Chun Yang Kun Zhou Baozhong Shen Hongyan Liang Xiaofeng Jiang Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy International Journal of Endocrinology |
| title | Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy |
| title_full | Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy |
| title_fullStr | Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy |
| title_full_unstemmed | Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy |
| title_short | Stability of miR-126 in Urine and Its Potential as a Biomarker for Renal Endothelial Injury with Diabetic Nephropathy |
| title_sort | stability of mir 126 in urine and its potential as a biomarker for renal endothelial injury with diabetic nephropathy |
| url | http://dx.doi.org/10.1155/2014/393109 |
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