Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue
Domoic acid (DA), a neurotoxic amino acid produced by diatoms, is the main cause of amnesic shellfish poisoning (ASP). In this work, we propose a very simple and fast analytical method to determine DA in mussel tissue. The method consists of two consecutive extractions and requires no purification s...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2016/8404092 |
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| author | Elena Barbaro Roberta Zangrando Carlo Barbante Andrea Gambaro |
| author_facet | Elena Barbaro Roberta Zangrando Carlo Barbante Andrea Gambaro |
| author_sort | Elena Barbaro |
| collection | DOAJ |
| description | Domoic acid (DA), a neurotoxic amino acid produced by diatoms, is the main cause of amnesic shellfish poisoning (ASP). In this work, we propose a very simple and fast analytical method to determine DA in mussel tissue. The method consists of two consecutive extractions and requires no purification steps, due to a reduction of the extraction of the interfering species and the application of very sensitive and selective HILIC-MS/MS method. The procedural method was validated through the estimation of trueness, extract yield, precision, detection, and quantification limits of analytical method. The sample preparation was also evaluated through qualitative and quantitative evaluations of the matrix effect. These evaluations were conducted both on the DA-free matrix spiked with known DA concentration and on the reference certified material (RCM). We developed a very selective LC-MS/MS method with a very low value of method detection limit (9 ng g−1) without cleanup steps. |
| format | Article |
| id | doaj-art-0bb03d03de644977ad8c1b5d83ce72cd |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-0bb03d03de644977ad8c1b5d83ce72cd2025-02-03T01:11:49ZengWileyThe Scientific World Journal2356-61401537-744X2016-01-01201610.1155/2016/84040928404092Fast and Sensitive Method for Determination of Domoic Acid in Mussel TissueElena Barbaro0Roberta Zangrando1Carlo Barbante2Andrea Gambaro3Department of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Via Torino 155, Mestre, 30170 Venice, ItalyInstitute for the Dynamics of Environmental Processes, CNR, Via Torino 155, Mestre, 30170 Venice, ItalyInstitute for the Dynamics of Environmental Processes, CNR, Via Torino 155, Mestre, 30170 Venice, ItalyDepartment of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Via Torino 155, Mestre, 30170 Venice, ItalyDomoic acid (DA), a neurotoxic amino acid produced by diatoms, is the main cause of amnesic shellfish poisoning (ASP). In this work, we propose a very simple and fast analytical method to determine DA in mussel tissue. The method consists of two consecutive extractions and requires no purification steps, due to a reduction of the extraction of the interfering species and the application of very sensitive and selective HILIC-MS/MS method. The procedural method was validated through the estimation of trueness, extract yield, precision, detection, and quantification limits of analytical method. The sample preparation was also evaluated through qualitative and quantitative evaluations of the matrix effect. These evaluations were conducted both on the DA-free matrix spiked with known DA concentration and on the reference certified material (RCM). We developed a very selective LC-MS/MS method with a very low value of method detection limit (9 ng g−1) without cleanup steps.http://dx.doi.org/10.1155/2016/8404092 |
| spellingShingle | Elena Barbaro Roberta Zangrando Carlo Barbante Andrea Gambaro Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue The Scientific World Journal |
| title | Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue |
| title_full | Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue |
| title_fullStr | Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue |
| title_full_unstemmed | Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue |
| title_short | Fast and Sensitive Method for Determination of Domoic Acid in Mussel Tissue |
| title_sort | fast and sensitive method for determination of domoic acid in mussel tissue |
| url | http://dx.doi.org/10.1155/2016/8404092 |
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