Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications
Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of re...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.3233/ACP-130075 |
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| _version_ | 1832560087010377728 |
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| author | Tieqiao Zhang Samantha Osborn Chloe Brandow Denis Dwyre Ralph Green Stephen Lane Sebastian Wachsmann-Hogiu |
| author_facet | Tieqiao Zhang Samantha Osborn Chloe Brandow Denis Dwyre Ralph Green Stephen Lane Sebastian Wachsmann-Hogiu |
| author_sort | Tieqiao Zhang |
| collection | DOAJ |
| description | Structured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy. |
| format | Article |
| id | doaj-art-0ba90ca8dd04425fbee615b4d99aa9b5 |
| institution | Kabale University |
| issn | 2210-7177 2210-7185 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-0ba90ca8dd04425fbee615b4d99aa9b52025-02-03T01:28:24ZengWileyAnalytical Cellular Pathology2210-71772210-71852013-01-01361-2273510.3233/ACP-130075Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology ApplicationsTieqiao Zhang0Samantha Osborn1Chloe Brandow2Denis Dwyre3Ralph Green4Stephen Lane5Sebastian Wachsmann-Hogiu6Center for Biophotonics, Science and Technology, University of California – Davis, Sacramento, CA, USACenter for Biophotonics, Science and Technology, University of California – Davis, Sacramento, CA, USACenter for Biophotonics, Science and Technology, University of California – Davis, Sacramento, CA, USADepartment of Pathology and Laboratory Medicine, School of Medicine, University of California – Davis, Sacramento, CA, USADepartment of Pathology and Laboratory Medicine, School of Medicine, University of California – Davis, Sacramento, CA, USACenter for Biophotonics, Science and Technology, University of California – Davis, Sacramento, CA, USACenter for Biophotonics, Science and Technology, University of California – Davis, Sacramento, CA, USAStructured illumination fluorescence microscopy utilizes interfering light and the moiré effect to enhance spatial resolution to about a half of that of conventional light microscopy, i.e. approximately 90 nm. In addition to the enhancement in the x and y directions, it also allows enhancement of resolution in the z- direction by the same factor of two (to approximately 220 nm), making it a powerful tool for 3-D morphology studies of fluorescently labeled cells or thin tissue sections. In this report, we applied this technique to several types of blood cells that are commonly seen in hematopathology. Compared with standard brightfield and ordinary fluorescence microscopy images, the 3-D morphology results clearly reveal the morphological features of different types of normal blood cells. We have also used this technique to evaluate morphologies of abnormal erythrocytes and compare them with those recorded on normal cells. The results give a very intuitive presentation of morphological structures of erythrocytes with great details. This research illustrates the potential of this technique to be used in hematology and cyto-pathology studies aimed at identifying nanometer-sized features that cannot be distinguished otherwise with conventional optical microscopy.http://dx.doi.org/10.3233/ACP-130075 |
| spellingShingle | Tieqiao Zhang Samantha Osborn Chloe Brandow Denis Dwyre Ralph Green Stephen Lane Sebastian Wachsmann-Hogiu Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications Analytical Cellular Pathology |
| title | Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications |
| title_full | Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications |
| title_fullStr | Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications |
| title_full_unstemmed | Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications |
| title_short | Structured Illumination-Based Super-Resolution Optical Microscopy for Hemato- and Cyto-Pathology Applications |
| title_sort | structured illumination based super resolution optical microscopy for hemato and cyto pathology applications |
| url | http://dx.doi.org/10.3233/ACP-130075 |
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