CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms

CAM/TMA-DPH as a promising alternative to SYTO9/PI for cell viability assessment in bacterial biofilms

IntroductionAccurately assessing biofilm viability is essential for evaluating both biofilm formation and the efficacy of antibacterial treatments. Traditional SYTO9 and propidium iodide (PI) live/dead staining in biofilm viability assays often ace challenges due to non-specific staining, limiting...

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Bibliographic Details
Main Authors: Tinatini Tchatchiashvili, Mateusz Jundzill, Mike Marquet, Kamran A. Mirza, Mathias W. Pletz, Oliwia Makarewicz, Lara Thieme
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
biofilm
viability
staining
metabolism
calcein acetoxymethyl
live/dead assay
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2024.1508016/full
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