Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro
Human adipose tissue-stromal derived cells (ASCs) are considered a perspective tool for regenerative medicine. Depending on the application mode ASC/allogeneic immune cell interaction can occur in the systemic circulation under plenty high concentrations of O2 and in target tissues at lower O2 level...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2016-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/4726267 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551022217658368 |
|---|---|
| author | Polina I. Bobyleva Elena R. Andreeva Aleksandra N. Gornostaeva Ludmila B. Buravkova |
| author_facet | Polina I. Bobyleva Elena R. Andreeva Aleksandra N. Gornostaeva Ludmila B. Buravkova |
| author_sort | Polina I. Bobyleva |
| collection | DOAJ |
| description | Human adipose tissue-stromal derived cells (ASCs) are considered a perspective tool for regenerative medicine. Depending on the application mode ASC/allogeneic immune cell interaction can occur in the systemic circulation under plenty high concentrations of O2 and in target tissues at lower O2 levels. Here we examined the effects of allogeneic PHA-stimulated peripheral blood mononuclear cells (PBMCs) on ASCs under ambient (20%) oxygen and “physiological” hypoxia (5% O2). As revealed with microarray analysis ASCs under 20% O2 were more affected by activated PBMCs, which was manifested in differential expression of more than 300 genes, whereas under 5% O2 only 140 genes were changed. Altered gene pattern was only partly overlapped at different O2 conditions. Under O2 ASCs retained their proliferative and differentiative capacities, mesenchymal phenotype, and intracellular organelle’ state. ASCs were proinflammatory activated on transcription level that was confirmed by their ability to suppress activation and proliferation of mitogen-stimulated PBMCs. ASC/PBMCs interaction resulted in anti-inflammatory shift of paracrine mediators in conditioning medium with significant increase of immunosuppressive LIF level. Our data indicated that under both ambient and tissue-related O2 ASCs possessed immunosuppressive potential and maintained functional activity. Under “physiological” hypoxia ASCs were less susceptible to “priming” by allogeneic mitogen-activated PBMCs. |
| format | Article |
| id | doaj-art-0ba4fedafa354809a09249c90d9d7402 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0ba4fedafa354809a09249c90d9d74022025-02-03T06:05:09ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/47262674726267Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In VitroPolina I. Bobyleva0Elena R. Andreeva1Aleksandra N. Gornostaeva2Ludmila B. Buravkova3Institute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse 76a, Moscow 123007, RussiaInstitute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse 76a, Moscow 123007, RussiaInstitute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse 76a, Moscow 123007, RussiaInstitute of Biomedical Problems, Russian Academy of Sciences, Khoroshevskoye Shosse 76a, Moscow 123007, RussiaHuman adipose tissue-stromal derived cells (ASCs) are considered a perspective tool for regenerative medicine. Depending on the application mode ASC/allogeneic immune cell interaction can occur in the systemic circulation under plenty high concentrations of O2 and in target tissues at lower O2 levels. Here we examined the effects of allogeneic PHA-stimulated peripheral blood mononuclear cells (PBMCs) on ASCs under ambient (20%) oxygen and “physiological” hypoxia (5% O2). As revealed with microarray analysis ASCs under 20% O2 were more affected by activated PBMCs, which was manifested in differential expression of more than 300 genes, whereas under 5% O2 only 140 genes were changed. Altered gene pattern was only partly overlapped at different O2 conditions. Under O2 ASCs retained their proliferative and differentiative capacities, mesenchymal phenotype, and intracellular organelle’ state. ASCs were proinflammatory activated on transcription level that was confirmed by their ability to suppress activation and proliferation of mitogen-stimulated PBMCs. ASC/PBMCs interaction resulted in anti-inflammatory shift of paracrine mediators in conditioning medium with significant increase of immunosuppressive LIF level. Our data indicated that under both ambient and tissue-related O2 ASCs possessed immunosuppressive potential and maintained functional activity. Under “physiological” hypoxia ASCs were less susceptible to “priming” by allogeneic mitogen-activated PBMCs.http://dx.doi.org/10.1155/2016/4726267 |
| spellingShingle | Polina I. Bobyleva Elena R. Andreeva Aleksandra N. Gornostaeva Ludmila B. Buravkova Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro Stem Cells International |
| title | Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro |
| title_full | Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro |
| title_fullStr | Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro |
| title_full_unstemmed | Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro |
| title_short | Tissue-Related Hypoxia Attenuates Proinflammatory Effects of Allogeneic PBMCs on Adipose-Derived Stromal Cells In Vitro |
| title_sort | tissue related hypoxia attenuates proinflammatory effects of allogeneic pbmcs on adipose derived stromal cells in vitro |
| url | http://dx.doi.org/10.1155/2016/4726267 |
| work_keys_str_mv | AT polinaibobyleva tissuerelatedhypoxiaattenuatesproinflammatoryeffectsofallogeneicpbmcsonadiposederivedstromalcellsinvitro AT elenarandreeva tissuerelatedhypoxiaattenuatesproinflammatoryeffectsofallogeneicpbmcsonadiposederivedstromalcellsinvitro AT aleksandrangornostaeva tissuerelatedhypoxiaattenuatesproinflammatoryeffectsofallogeneicpbmcsonadiposederivedstromalcellsinvitro AT ludmilabburavkova tissuerelatedhypoxiaattenuatesproinflammatoryeffectsofallogeneicpbmcsonadiposederivedstromalcellsinvitro |