Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15

Microbial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An al...

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Main Authors: El-Sayed E. Mostafa, Moataza M. Saad, Hassan M. Awad, Mohsen H. Selim, Helmy M. Hassan
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:E-Journal of Chemistry
Online Access:http://dx.doi.org/10.1155/2012/168540
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author El-Sayed E. Mostafa
Moataza M. Saad
Hassan M. Awad
Mohsen H. Selim
Helmy M. Hassan
author_facet El-Sayed E. Mostafa
Moataza M. Saad
Hassan M. Awad
Mohsen H. Selim
Helmy M. Hassan
author_sort El-Sayed E. Mostafa
collection DOAJ
description Microbial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An alkaline protease producer strain NRC-15 was isolated from Egyptian soil sample. The cultural, morphological, physiological characters and chemotaxonomic evidence strongly indicated that the NRC-15 strain represents a novel species of the genus Streptomyces, hence the name Strptomyces pseudogrisiolus NRC-15. The culture conditions for higher protease production by NRC-15 were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% glucose, 1% yeast extract, 6% NaCl and 100 μmol/L of Tween 20, initial pH 9.0 at 50 °C for 96 h. The current results confirm that for this strain, a great ability to produce alkaline proteases, which supports the use of applications in industry.
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issn 0973-4945
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language English
publishDate 2012-01-01
publisher Wiley
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series E-Journal of Chemistry
spelling doaj-art-0b9f375c996f42f3adae0a96a568913e2025-02-03T01:11:03ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-019294996110.1155/2012/168540Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15El-Sayed E. Mostafa0Moataza M. Saad1Hassan M. Awad2Mohsen H. Selim3Helmy M. Hassan4Microbial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptMicrobial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptChemistry of Natural and Microbial Products Dept., National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, MalaysiaMicrobial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptMicrobial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An alkaline protease producer strain NRC-15 was isolated from Egyptian soil sample. The cultural, morphological, physiological characters and chemotaxonomic evidence strongly indicated that the NRC-15 strain represents a novel species of the genus Streptomyces, hence the name Strptomyces pseudogrisiolus NRC-15. The culture conditions for higher protease production by NRC-15 were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% glucose, 1% yeast extract, 6% NaCl and 100 μmol/L of Tween 20, initial pH 9.0 at 50 °C for 96 h. The current results confirm that for this strain, a great ability to produce alkaline proteases, which supports the use of applications in industry.http://dx.doi.org/10.1155/2012/168540
spellingShingle El-Sayed E. Mostafa
Moataza M. Saad
Hassan M. Awad
Mohsen H. Selim
Helmy M. Hassan
Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
E-Journal of Chemistry
title Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
title_full Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
title_fullStr Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
title_full_unstemmed Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
title_short Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
title_sort optimization conditions of extracellular proteases production from a newly isolated streptomyces pseudogrisiolus nrc 15
url http://dx.doi.org/10.1155/2012/168540
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