Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15
Microbial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An al...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2012/168540 |
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| author | El-Sayed E. Mostafa Moataza M. Saad Hassan M. Awad Mohsen H. Selim Helmy M. Hassan |
| author_facet | El-Sayed E. Mostafa Moataza M. Saad Hassan M. Awad Mohsen H. Selim Helmy M. Hassan |
| author_sort | El-Sayed E. Mostafa |
| collection | DOAJ |
| description | Microbial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An alkaline protease producer strain NRC-15 was isolated from Egyptian soil sample. The cultural, morphological, physiological characters and chemotaxonomic evidence strongly indicated that the NRC-15 strain represents a novel species of the genus Streptomyces, hence the name Strptomyces pseudogrisiolus NRC-15. The culture conditions for higher protease production by NRC-15 were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% glucose, 1% yeast extract, 6% NaCl and 100 μmol/L of Tween 20, initial pH 9.0 at 50 °C for 96 h. The current results confirm that for this strain, a great ability to produce alkaline proteases, which supports the use of applications in industry. |
| format | Article |
| id | doaj-art-0b9f375c996f42f3adae0a96a568913e |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-0b9f375c996f42f3adae0a96a568913e2025-02-03T01:11:03ZengWileyE-Journal of Chemistry0973-49452090-98102012-01-019294996110.1155/2012/168540Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15El-Sayed E. Mostafa0Moataza M. Saad1Hassan M. Awad2Mohsen H. Selim3Helmy M. Hassan4Microbial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptMicrobial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptChemistry of Natural and Microbial Products Dept., National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptInstitute of Bioproduct Development, Universiti Teknologi Malaysia (UTM), 81310 Skudai, Johor, MalaysiaMicrobial Chemistry Department, National Research Centre (NRC), El-Bohouth Street, Dokki, P.O. Box 12622, Cairo, EgyptMicrobial protease represents the most important industrial enzymes, which have an active role in biotechnological processes. The objective of this study was to isolate new strain of Streptomyces that produce proteolytic enzymes with novel properties and the development of the low-cost medium. An alkaline protease producer strain NRC-15 was isolated from Egyptian soil sample. The cultural, morphological, physiological characters and chemotaxonomic evidence strongly indicated that the NRC-15 strain represents a novel species of the genus Streptomyces, hence the name Strptomyces pseudogrisiolus NRC-15. The culture conditions for higher protease production by NRC-15 were optimized with respect to carbon and nitrogen sources, metal ions, pH and temperature. Maximum protease production was obtained in the medium supplemented with 1% glucose, 1% yeast extract, 6% NaCl and 100 μmol/L of Tween 20, initial pH 9.0 at 50 °C for 96 h. The current results confirm that for this strain, a great ability to produce alkaline proteases, which supports the use of applications in industry.http://dx.doi.org/10.1155/2012/168540 |
| spellingShingle | El-Sayed E. Mostafa Moataza M. Saad Hassan M. Awad Mohsen H. Selim Helmy M. Hassan Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15 E-Journal of Chemistry |
| title | Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15 |
| title_full | Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15 |
| title_fullStr | Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15 |
| title_full_unstemmed | Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15 |
| title_short | Optimization Conditions of Extracellular Proteases Production from a Newly Isolated Streptomyces Pseudogrisiolus NRC-15 |
| title_sort | optimization conditions of extracellular proteases production from a newly isolated streptomyces pseudogrisiolus nrc 15 |
| url | http://dx.doi.org/10.1155/2012/168540 |
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