Quality control checkpoints for high throughput DNA methylation measurement using the human MethylationEPICv1 array: application to formalin-fixed paraffin embedded prostate tissue

Quality control checkpoints for high throughput DNA methylation measurement using the human MethylationEPICv1 array: application to formalin-fixed paraffin embedded prostate tissue

Abstract Objective The performance of FFPE tissue-derived DNA on the MethylationEpicV1.0 array can be unpredictable as the protocol only has two quality checks; checkpoint 1 for DNA quantity and checkpoint 2 for DNA quality assessment. We sought to incorporate a third, previously developed bisulfite...

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Bibliographic Details
Main Authors: Robert L. O’Reilly, Fleur Hammet, Neil O’Callaghan, Robert J. MacInnis, Damien Bolton, Graham G. Giles, Pierre-Antoine Dugué, Melissa C. Southey
Format: Article
Language:English
Published: BMC 2025-07-01
Series:BMC Research Notes
Subjects:
FFPE tissue
Human methylationepic Beadchip
Bisulfite conversion
Quality control
FFPE tissue-derived DNA
Online Access:https://doi.org/10.1186/s13104-025-07221-3
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