HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts
A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, wit...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2020/8851285 |
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| author | Sung-Ho Lee Seong-Wook Seo Seung-Yub Song Deuk-Sil Oh Jung-Hyun Shim Goo Yoon Ye-Yong Choi Do-Kyung Kim Jae-Sung Kim In-Soo Yoon Seung-Sik Cho |
| author_facet | Sung-Ho Lee Seong-Wook Seo Seung-Yub Song Deuk-Sil Oh Jung-Hyun Shim Goo Yoon Ye-Yong Choi Do-Kyung Kim Jae-Sung Kim In-Soo Yoon Seung-Sik Cho |
| author_sort | Sung-Ho Lee |
| collection | DOAJ |
| description | A method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts. |
| format | Article |
| id | doaj-art-0b84bd56c62f4a07bcef1e898f6cd2fe |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-0b84bd56c62f4a07bcef1e898f6cd2fe2025-02-03T05:54:25ZengWileyJournal of Food Quality0146-94281745-45572020-01-01202010.1155/2020/88512858851285HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans ExtractsSung-Ho Lee0Seong-Wook Seo1Seung-Yub Song2Deuk-Sil Oh3Jung-Hyun Shim4Goo Yoon5Ye-Yong Choi6Do-Kyung Kim7Jae-Sung Kim8In-Soo Yoon9Seung-Sik Cho10College of Pharmacy, Mokpo National University, 1666 Yeongsan-ro, Muan-gun, Jeollanam-do 58554, Republic of KoreaCollege of Pharmacy, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of KoreaCollege of Pharmacy, Mokpo National University, 1666 Yeongsan-ro, Muan-gun, Jeollanam-do 58554, Republic of KoreaJeollanam-do Forest Resource Research Institute, 7 Dado-ro, Sanpo-myeon, Naju-si, Jeollanam-do 58213, Republic of KoreaCollege of Pharmacy, Mokpo National University, 1666 Yeongsan-ro, Muan-gun, Jeollanam-do 58554, Republic of KoreaCollege of Pharmacy, Mokpo National University, 1666 Yeongsan-ro, Muan-gun, Jeollanam-do 58554, Republic of KoreaResearch and Development Institute, CY Pharmaceutical Co., 340 Nonhyeon-ro, Gangnam-gu, Seoul 06227, Republic of KoreaSchool of Dentistry, Chosun University, Gwangju, 460 Iksan-daero, Iksan-si, Jeollabuk-do 54538, Republic of KoreaSchool of Dentistry, Chosun University, Gwangju, 460 Iksan-daero, Iksan-si, Jeollabuk-do 54538, Republic of KoreaCollege of Pharmacy, Pusan National University, 2 Busandaehak-ro 63beon-gil, Geumjeong-gu, Busan 46241, Republic of KoreaCollege of Pharmacy, Mokpo National University, 1666 Yeongsan-ro, Muan-gun, Jeollanam-do 58554, Republic of KoreaA method for the separation and quantification of acteoside by reverse-phase high-performance liquid chromatography (HPLC) was developed and validated. Hot water and ethanolic extracts of Osmanthus fragrans leaves and flowers were analyzed for acteoside content. Excellent linearity was obtained, with an r2 higher than 0.999. The precision, specificity, and accuracy of our method were excellent, suggesting that it can be conveniently used for the quantification of acteoside in the crude extract of O. fragrans. The hot water and ethanol extracts were analyzed, and their biological activities were tested. The extraction yields, marker (acteoside) contents, and antioxidant activities of the leaf and flower extracts were analyzed. The antioxidant activity was confirmed by measuring the 2,2-diphenyl-2-picrylhydrazyl radical scavenging activity, reducing power, and total phenolic content. The acteoside content tended to be higher in the 100% ethanol extract of O. fragrans compared to those with the other extraction conditions tested. Overall, almost all extracts prepared with ethanolic solvents tended to produce better antioxidant activity than those prepared with hot water. These results suggest that the ethanolic extract of O. fragrans could serve as a potential antioxidant and anti-inflammatory pharmaceutical source, and our validated method would be useful for the quality control of O. fragrans extracts.http://dx.doi.org/10.1155/2020/8851285 |
| spellingShingle | Sung-Ho Lee Seong-Wook Seo Seung-Yub Song Deuk-Sil Oh Jung-Hyun Shim Goo Yoon Ye-Yong Choi Do-Kyung Kim Jae-Sung Kim In-Soo Yoon Seung-Sik Cho HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts Journal of Food Quality |
| title | HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts |
| title_full | HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts |
| title_fullStr | HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts |
| title_full_unstemmed | HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts |
| title_short | HPLC Analysis and Antioxidant Evaluation of Acteoside-Rich Osmanthus fragrans Extracts |
| title_sort | hplc analysis and antioxidant evaluation of acteoside rich osmanthus fragrans extracts |
| url | http://dx.doi.org/10.1155/2020/8851285 |
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