Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023
Abstract Background Staphylococcus aureus is one of the most common pathogens that colonizes human skin/mucous membranes, where it causes local infection that can progress to invasive infection, resulting in high morbidity and mortality worldwide. This study aimed to investigate the antibiotic susce...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12866-025-03758-2 |
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| author | Li Liu Ziyi Yan Fumei He Jie Chen Linghan Kuang Xingxin Liu Yali Cui Xia Wang Chenglin Miao Hong Li Yongmei Jiang |
| author_facet | Li Liu Ziyi Yan Fumei He Jie Chen Linghan Kuang Xingxin Liu Yali Cui Xia Wang Chenglin Miao Hong Li Yongmei Jiang |
| author_sort | Li Liu |
| collection | DOAJ |
| description | Abstract Background Staphylococcus aureus is one of the most common pathogens that colonizes human skin/mucous membranes, where it causes local infection that can progress to invasive infection, resulting in high morbidity and mortality worldwide. This study aimed to investigate the antibiotic susceptibility and molecular characteristics of invasive S. aureus in children and women in Southwest China from 2018 to 2023 to provide novel insights helpful in preventing and treating S. aureus infections. Methods The demographic and clinical characteristics of patients with invasive S. aureus infection were collected and analyzed. Next-generation sequencing (NGS) and sequence analysis techniques were used to determine the molecular epidemiological characteristics of the S. aureus isolates, and the microdilution broth method was used for antimicrobial susceptibility testing. Results A total of 108 invasive S. aureus isolates, 29 methicillin-resistant S. aureus (MRSA) isolates and 79 methicillin- susceptible S. aureus (MSSA) isolates, were included. The isolates had the highest rate of resistance to PEN, at 91.67%, with all the MRSA isolates being resistant; the next highest resistance was to ERY and CLI, both at 65.74%. A total of 32 STs (including 8 novel STs) were detected and divided into 10 CCs. Moreover, 45 spa types were also detected. The main STs were ST22 (17.59%) and ST59 (15.74%), and the main CCs were CC59 (21.30%) and CC22 (19.44%). The most prevalent spa types were t309 and t437, both at 14.81%, and the SCCmec type could be assigned to two categories: IV (62.07%) and V (34.48%). Among the 29 MRSA isolates tested, CC59-IV-t437 (34.48%) and CC59-V-t437 (13.79%) were the main lineages, and among the 79 MSSA isolates, CC22-t309 (18.99%), CC1-t189 (10.13%), and CC5-t002 (7.60%) were the main lineages. Except for SXT, the resistance rates of the 29 MRSA isolates were greater than those of the MSSA isolates. Most isolates carried common virulence genes, among which the carriage rate of pvl reached 33.33%. Conclusions This study provides valuable information, including the prevalence, molecular characteristics and antimicrobial resistance of S. aureus isolates that cause invasive infectious diseases in Southwest China, and the findings may advance the prevention and treatment of S. aureus infections. |
| format | Article |
| id | doaj-art-0b53cb8bd31b4684833230dd15f57358 |
| institution | Kabale University |
| issn | 1471-2180 |
| language | English |
| publishDate | 2025-01-01 |
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| series | BMC Microbiology |
| spelling | doaj-art-0b53cb8bd31b4684833230dd15f573582025-02-02T12:11:12ZengBMCBMC Microbiology1471-21802025-01-0125111310.1186/s12866-025-03758-2Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023Li Liu0Ziyi Yan1Fumei He2Jie Chen3Linghan Kuang4Xingxin Liu5Yali Cui6Xia Wang7Chenglin Miao8Hong Li9Yongmei Jiang10Department of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityCollege of Computer Science, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityKey Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, West China Second University Hospital, Sichuan UniversityDepartment of Laboratory Medicine, West China Second University Hospital, Sichuan UniversityAbstract Background Staphylococcus aureus is one of the most common pathogens that colonizes human skin/mucous membranes, where it causes local infection that can progress to invasive infection, resulting in high morbidity and mortality worldwide. This study aimed to investigate the antibiotic susceptibility and molecular characteristics of invasive S. aureus in children and women in Southwest China from 2018 to 2023 to provide novel insights helpful in preventing and treating S. aureus infections. Methods The demographic and clinical characteristics of patients with invasive S. aureus infection were collected and analyzed. Next-generation sequencing (NGS) and sequence analysis techniques were used to determine the molecular epidemiological characteristics of the S. aureus isolates, and the microdilution broth method was used for antimicrobial susceptibility testing. Results A total of 108 invasive S. aureus isolates, 29 methicillin-resistant S. aureus (MRSA) isolates and 79 methicillin- susceptible S. aureus (MSSA) isolates, were included. The isolates had the highest rate of resistance to PEN, at 91.67%, with all the MRSA isolates being resistant; the next highest resistance was to ERY and CLI, both at 65.74%. A total of 32 STs (including 8 novel STs) were detected and divided into 10 CCs. Moreover, 45 spa types were also detected. The main STs were ST22 (17.59%) and ST59 (15.74%), and the main CCs were CC59 (21.30%) and CC22 (19.44%). The most prevalent spa types were t309 and t437, both at 14.81%, and the SCCmec type could be assigned to two categories: IV (62.07%) and V (34.48%). Among the 29 MRSA isolates tested, CC59-IV-t437 (34.48%) and CC59-V-t437 (13.79%) were the main lineages, and among the 79 MSSA isolates, CC22-t309 (18.99%), CC1-t189 (10.13%), and CC5-t002 (7.60%) were the main lineages. Except for SXT, the resistance rates of the 29 MRSA isolates were greater than those of the MSSA isolates. Most isolates carried common virulence genes, among which the carriage rate of pvl reached 33.33%. Conclusions This study provides valuable information, including the prevalence, molecular characteristics and antimicrobial resistance of S. aureus isolates that cause invasive infectious diseases in Southwest China, and the findings may advance the prevention and treatment of S. aureus infections.https://doi.org/10.1186/s12866-025-03758-2Staphylococcus aureusInvasive infectionWhole-genome sequencingMultilocus sequence typingSpaAntibiotic resistance |
| spellingShingle | Li Liu Ziyi Yan Fumei He Jie Chen Linghan Kuang Xingxin Liu Yali Cui Xia Wang Chenglin Miao Hong Li Yongmei Jiang Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023 BMC Microbiology Staphylococcus aureus Invasive infection Whole-genome sequencing Multilocus sequence typing Spa Antibiotic resistance |
| title | Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023 |
| title_full | Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023 |
| title_fullStr | Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023 |
| title_full_unstemmed | Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023 |
| title_short | Antibiotic susceptibility and molecular characterization based on whole-genome sequencing of Staphylococcus aureus causing invasive infection in children and women living in Southwest China during 2018–2023 |
| title_sort | antibiotic susceptibility and molecular characterization based on whole genome sequencing of staphylococcus aureus causing invasive infection in children and women living in southwest china during 2018 2023 |
| topic | Staphylococcus aureus Invasive infection Whole-genome sequencing Multilocus sequence typing Spa Antibiotic resistance |
| url | https://doi.org/10.1186/s12866-025-03758-2 |
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