Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition
Abstract The fecundity of Nilaparvata lugens (brown planthopper) is influenced by trehalase (TRE). To investigate the mechanism by which trehalose affects the reproduction of N. lugens, we conducted a comparative transcriptomic and metabolomic analysis of the ovaries of N. lugens following injection...
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BMC
2025-02-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-025-11268-8 |
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| author | Yongkang Liu Fan Yang Sijing Wan Xianzhong Wang Liwen Guan Yan Li Caidi Xu Binghua Xie Shigui Wang Xiao-Ling Tan Bin Tang |
| author_facet | Yongkang Liu Fan Yang Sijing Wan Xianzhong Wang Liwen Guan Yan Li Caidi Xu Binghua Xie Shigui Wang Xiao-Ling Tan Bin Tang |
| author_sort | Yongkang Liu |
| collection | DOAJ |
| description | Abstract The fecundity of Nilaparvata lugens (brown planthopper) is influenced by trehalase (TRE). To investigate the mechanism by which trehalose affects the reproduction of N. lugens, we conducted a comparative transcriptomic and metabolomic analysis of the ovaries of N. lugens following injection with dsTREs and validamycin (a TRE inhibitor). The results revealed that 844 differentially expressed genes (DEGs) were identified between the dsGFP and dsTREs injection groups, with 317 up-regulated genes and 527 down-regulated genes. Additionally, 1451 DEGs were identified between the water and validamycin injection groups, with 637 up-regulated genes and 814 down-regulated genes. The total number of DEGs identified between the two comparison groups was 236. The overlapping DEGs were implicated in various biological processes, including protein metabolism, fatty acid metabolism, AMPK signaling, mTOR signaling, insulin/insulin-like growth factor signaling (IIS), the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and the cellular process of meiosis in oocytes. These results suggest that the inhibition of TRE expression may lead to alterations in ovarian nutrient and energy metabolism by modulating glucose transport and affecting amino acid metabolic pathways. These alterations may influence the reproduction of N. lugens by modulating reproductive regulatory signals. These findings provide robust evidence supporting the mechanism through which trehalase inhibition reduces the reproductive capacity of N. lugens. |
| format | Article |
| id | doaj-art-0b238d4e93c247d980de58ba353105b9 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-0b238d4e93c247d980de58ba353105b92025-02-02T12:10:18ZengBMCBMC Genomics1471-21642025-02-0126111610.1186/s12864-025-11268-8Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibitionYongkang Liu0Fan Yang1Sijing Wan2Xianzhong Wang3Liwen Guan4Yan Li5Caidi Xu6Binghua Xie7Shigui Wang8Xiao-Ling Tan9Bin Tang10College of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityChinese Education Modernization Research Institute of Hangzhou Normal University, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityCollege of Life and Environmental Sciences, Hangzhou Normal UniversityState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesCollege of Life and Environmental Sciences, Hangzhou Normal UniversityAbstract The fecundity of Nilaparvata lugens (brown planthopper) is influenced by trehalase (TRE). To investigate the mechanism by which trehalose affects the reproduction of N. lugens, we conducted a comparative transcriptomic and metabolomic analysis of the ovaries of N. lugens following injection with dsTREs and validamycin (a TRE inhibitor). The results revealed that 844 differentially expressed genes (DEGs) were identified between the dsGFP and dsTREs injection groups, with 317 up-regulated genes and 527 down-regulated genes. Additionally, 1451 DEGs were identified between the water and validamycin injection groups, with 637 up-regulated genes and 814 down-regulated genes. The total number of DEGs identified between the two comparison groups was 236. The overlapping DEGs were implicated in various biological processes, including protein metabolism, fatty acid metabolism, AMPK signaling, mTOR signaling, insulin/insulin-like growth factor signaling (IIS), the tricarboxylic acid (TCA) cycle, oxidative phosphorylation, and the cellular process of meiosis in oocytes. These results suggest that the inhibition of TRE expression may lead to alterations in ovarian nutrient and energy metabolism by modulating glucose transport and affecting amino acid metabolic pathways. These alterations may influence the reproduction of N. lugens by modulating reproductive regulatory signals. These findings provide robust evidence supporting the mechanism through which trehalase inhibition reduces the reproductive capacity of N. lugens.https://doi.org/10.1186/s12864-025-11268-8Nilaparvata lugensTranscriptomicMetabolomicsOvary developmentTrehalase |
| spellingShingle | Yongkang Liu Fan Yang Sijing Wan Xianzhong Wang Liwen Guan Yan Li Caidi Xu Binghua Xie Shigui Wang Xiao-Ling Tan Bin Tang Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition BMC Genomics Nilaparvata lugens Transcriptomic Metabolomics Ovary development Trehalase |
| title | Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition |
| title_full | Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition |
| title_fullStr | Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition |
| title_full_unstemmed | Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition |
| title_short | Comparative transcriptomic and metabolomics analysis of ovary in Nilaparvata lugens after trehalase inhibition |
| title_sort | comparative transcriptomic and metabolomics analysis of ovary in nilaparvata lugens after trehalase inhibition |
| topic | Nilaparvata lugens Transcriptomic Metabolomics Ovary development Trehalase |
| url | https://doi.org/10.1186/s12864-025-11268-8 |
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