Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica)
Abstract Background The Japanese eel (Anguilla japonica) holds significant economic value in East Asia, but limitations in understanding its reproductive biology have hindered advancements in artificial breeding techniques. Previous research has primarily focused on conserved sex differentiation gen...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12864-025-11279-5 |
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| author | Jiangling Li Jingjie Liang Mengyang Wang Yuewen Jiang Wen Li Mingxi Huang Yan Huang Yangjie Xie Jianchun Chen Tiansheng Chen |
| author_facet | Jiangling Li Jingjie Liang Mengyang Wang Yuewen Jiang Wen Li Mingxi Huang Yan Huang Yangjie Xie Jianchun Chen Tiansheng Chen |
| author_sort | Jiangling Li |
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| description | Abstract Background The Japanese eel (Anguilla japonica) holds significant economic value in East Asia, but limitations in understanding its reproductive biology have hindered advancements in artificial breeding techniques. Previous research has primarily focused on conserved sex differentiation genes, offering limited insights into the broader molecular mechanisms driving gonadal development and sexual dimorphism. To address these limitations, this study aims to investigate key genes and pathways involved in gonadal development through a comprehensive transcriptomic analysis of male and female eel gonads. Results PacBio Iso-Seq and Illumina RNA-Seq technologies were combined to conduct a full-length transcriptome analysis of male and female Japanese eel gonads at a post-differentiation, pre-maturation stage. A total of 24,661 unigenes were identified in ovaries and 15,023 in testes, along with genomic regulatory elements such as transcription factors, simple sequence repeats, and long non-coding RNAs. Additionally, 1,210 differentially expressed genes were detected. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed significant pathways involved in cell cycle regulation, metabolic processes, apoptosis, and hormone activity. Notably, several reproductive-related genes, including bambi, ccnb1, cdc20, gdf9, prlh, ccdc39, chrebp, tspo, syce3, and ngb, demonstrated significant dimorphic expression in eel gonads. Conclusions This study provides valuable insights into the molecular mechanisms of gonadal differentiation and sexual dimorphism in Japanese eels. The findings expand the genetic resources available for the eel breeding industry and could facilitate the development of improved artificial breeding techniques focused on reproductive development. |
| format | Article |
| id | doaj-art-0b18e53b701b48a2ba8a04829598b906 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Genomics |
| spelling | doaj-art-0b18e53b701b48a2ba8a04829598b9062025-02-02T12:10:11ZengBMCBMC Genomics1471-21642025-01-0126111610.1186/s12864-025-11279-5Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica)Jiangling Li0Jingjie Liang1Mengyang Wang2Yuewen Jiang3Wen Li4Mingxi Huang5Yan Huang6Yangjie Xie7Jianchun Chen8Tiansheng Chen9State Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityCollege of Fisheries, Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affairs, Huazhong Agricultural UniversityState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityXiamen Institute of Marine and FisheriesState Key Laboratory of Mariculture Breeding; Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education；Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Fisheries College of Jimei UniversityAbstract Background The Japanese eel (Anguilla japonica) holds significant economic value in East Asia, but limitations in understanding its reproductive biology have hindered advancements in artificial breeding techniques. Previous research has primarily focused on conserved sex differentiation genes, offering limited insights into the broader molecular mechanisms driving gonadal development and sexual dimorphism. To address these limitations, this study aims to investigate key genes and pathways involved in gonadal development through a comprehensive transcriptomic analysis of male and female eel gonads. Results PacBio Iso-Seq and Illumina RNA-Seq technologies were combined to conduct a full-length transcriptome analysis of male and female Japanese eel gonads at a post-differentiation, pre-maturation stage. A total of 24,661 unigenes were identified in ovaries and 15,023 in testes, along with genomic regulatory elements such as transcription factors, simple sequence repeats, and long non-coding RNAs. Additionally, 1,210 differentially expressed genes were detected. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed significant pathways involved in cell cycle regulation, metabolic processes, apoptosis, and hormone activity. Notably, several reproductive-related genes, including bambi, ccnb1, cdc20, gdf9, prlh, ccdc39, chrebp, tspo, syce3, and ngb, demonstrated significant dimorphic expression in eel gonads. Conclusions This study provides valuable insights into the molecular mechanisms of gonadal differentiation and sexual dimorphism in Japanese eels. The findings expand the genetic resources available for the eel breeding industry and could facilitate the development of improved artificial breeding techniques focused on reproductive development.https://doi.org/10.1186/s12864-025-11279-5Japanese eel (Anguilla japonica)Transcriptome analysisTestisOvarySexual dimorphism |
| spellingShingle | Jiangling Li Jingjie Liang Mengyang Wang Yuewen Jiang Wen Li Mingxi Huang Yan Huang Yangjie Xie Jianchun Chen Tiansheng Chen Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) BMC Genomics Japanese eel (Anguilla japonica) Transcriptome analysis Testis Ovary Sexual dimorphism |
| title | Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) |
| title_full | Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) |
| title_fullStr | Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) |
| title_full_unstemmed | Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) |
| title_short | Full-length transcriptome analysis of male and female gonads in Japanese Eel (Anguilla japonica) |
| title_sort | full length transcriptome analysis of male and female gonads in japanese eel anguilla japonica |
| topic | Japanese eel (Anguilla japonica) Transcriptome analysis Testis Ovary Sexual dimorphism |
| url | https://doi.org/10.1186/s12864-025-11279-5 |
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