Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards
A fungus was isolated from ambrosia beetles collected using beetle traps in an apple orchard in Gunwi-gun, Daegu, South Korea. This fungal strain was termed ARI-24-A5, and was identified through morphological characterization and molecular phylogenetic analysis. After 8 d of incubation on potato dex...
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The Korean Society of Mycology
2024-12-01
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| Series: | 한국균학회지 |
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| Online Access: | https://www.kjmycology.or.kr/5204-16/ |
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| author | Ju-Heon Lee Youngsoo Kim Jong-Taek Park Dong-Hyuk Lee Hee-Young Jung |
| author_facet | Ju-Heon Lee Youngsoo Kim Jong-Taek Park Dong-Hyuk Lee Hee-Young Jung |
| author_sort | Ju-Heon Lee |
| collection | DOAJ |
| description | A fungus was isolated from ambrosia beetles collected using beetle traps in an apple orchard in Gunwi-gun, Daegu, South Korea. This fungal strain was termed ARI-24-A5, and was identified through morphological characterization and molecular phylogenetic analysis. After 8 d of incubation on potato dextrose agar (PDA), ARI-24-A5 exhibited gray-to-olive coloration, abundant aerial mycelia, and a colony diameter of 72.0–79.0 mm. Morphologically, the aleurioconidiophores formed monilioid chain structures, and the size of the aleurioconidia was 11.1 × 10.8 μm. For precise identification, molecular phylogenetic analysis was performed using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF1-α), small subunit of nuclear ribosomal RNA (SSU), and RNA polymerase II subunit 1 (RPB1) gene sequences. The overall analysis confirmed that ARI-24-A5 belongs to the genus Ambrosiella, which is known for its symbiotic relationship with ambrosia beetles. In the phylogenetic tree, ARI-24-A5 shared the same taxonomic position as A. catenulata and its morphological characteristics were consistent with those of this species. Therefore, ARI24-A5 was identified as A. catenulata, making this the first record of this species in South Korea. |
| format | Article |
| id | doaj-art-0aba6d94622a42f29e1524c7f5fa7e18 |
| institution | DOAJ |
| issn | 0253-651X 2383-5249 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | The Korean Society of Mycology |
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| series | 한국균학회지 |
| spelling | doaj-art-0aba6d94622a42f29e1524c7f5fa7e182025-08-20T03:10:46ZengThe Korean Society of Mycology한국균학회지0253-651X2383-52492024-12-0152438138910.4489/kjm.520416Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple OrchardsJu-Heon Lee0Youngsoo Kim1Jong-Taek Park2Dong-Hyuk Lee3Hee-Young Jung4Apple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, KoreaApple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, KoreaApple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, KoreaApple Research Center, National Institute of Horticultural & Herbal Science, Gunwi 43100, KoreaDepartment of Plant Medicine, Kyungpook National University, Daegu 41566, KoreaA fungus was isolated from ambrosia beetles collected using beetle traps in an apple orchard in Gunwi-gun, Daegu, South Korea. This fungal strain was termed ARI-24-A5, and was identified through morphological characterization and molecular phylogenetic analysis. After 8 d of incubation on potato dextrose agar (PDA), ARI-24-A5 exhibited gray-to-olive coloration, abundant aerial mycelia, and a colony diameter of 72.0–79.0 mm. Morphologically, the aleurioconidiophores formed monilioid chain structures, and the size of the aleurioconidia was 11.1 × 10.8 μm. For precise identification, molecular phylogenetic analysis was performed using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF1-α), small subunit of nuclear ribosomal RNA (SSU), and RNA polymerase II subunit 1 (RPB1) gene sequences. The overall analysis confirmed that ARI-24-A5 belongs to the genus Ambrosiella, which is known for its symbiotic relationship with ambrosia beetles. In the phylogenetic tree, ARI-24-A5 shared the same taxonomic position as A. catenulata and its morphological characteristics were consistent with those of this species. Therefore, ARI24-A5 was identified as A. catenulata, making this the first record of this species in South Korea.https://www.kjmycology.or.kr/5204-16/ambrosia beetleambrosiella catenulatakorean apple orchardsymbiotic fungi |
| spellingShingle | Ju-Heon Lee Youngsoo Kim Jong-Taek Park Dong-Hyuk Lee Hee-Young Jung Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards 한국균학회지 ambrosia beetle ambrosiella catenulata korean apple orchard symbiotic fungi |
| title | Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards |
| title_full | Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards |
| title_fullStr | Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards |
| title_full_unstemmed | Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards |
| title_short | Ambrosiella catenulata Isolated from Ambrosia Beetles in Korean Apple Orchards |
| title_sort | ambrosiella catenulata isolated from ambrosia beetles in korean apple orchards |
| topic | ambrosia beetle ambrosiella catenulata korean apple orchard symbiotic fungi |
| url | https://www.kjmycology.or.kr/5204-16/ |
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