An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae)
Abstract Premise Seaweeds are gaining substantial research interest, particularly for genomic applications, where high‐quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Applications in Plant Sciences |
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| Online Access: | https://doi.org/10.1002/aps3.11625 |
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| author | Riyad Hossen Myles Courtney Alasdair Sim Md Abdullah Al Kamran Khan Heroen Verbruggen Trevor Bringloe |
| author_facet | Riyad Hossen Myles Courtney Alasdair Sim Md Abdullah Al Kamran Khan Heroen Verbruggen Trevor Bringloe |
| author_sort | Riyad Hossen |
| collection | DOAJ |
| description | Abstract Premise Seaweeds are gaining substantial research interest, particularly for genomic applications, where high‐quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing protocols often lack versatility, leading to inconsistent outcomes across various materials and taxa, which highlights the need for a universal method for use with a variety of green seaweed samples. Methods and Results We optimized the conventional cetyltrimethylammonium bromide (CTAB) protocol for green seaweed DNA extraction. Our method, involving an initial sample treatment, lysis buffer adjustment, and enzyme incubation alterations, outperformed the conventional CTAB and commercial kits in terms of DNA yield and purity. Notably, the protocol's effectiveness was demonstrated across various green algal materials and preservation methods, and was tested with downstream applications with satisfactory results. Conclusions Our optimized CTAB protocol offers a reliable solution for high‐quality genomic DNA extraction from a wide variety of green seaweed samples. |
| format | Article |
| id | doaj-art-0a9c23ceff324272a67bc51932186a35 |
| institution | Kabale University |
| issn | 2168-0450 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj-art-0a9c23ceff324272a67bc51932186a352025-02-03T12:21:35ZengWileyApplications in Plant Sciences2168-04502025-01-01131n/an/a10.1002/aps3.11625An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae)Riyad Hossen0Myles Courtney1Alasdair Sim2Md Abdullah Al Kamran Khan3Heroen Verbruggen4Trevor Bringloe5School of Biosciences University of Melbourne Victoria AustraliaSchool of Biosciences University of Melbourne Victoria AustraliaSchool of Biosciences University of Melbourne Victoria AustraliaDepartment of Microbiology and Immunology at The Peter Doherty Institute University of Melbourne Victoria AustraliaSchool of Biosciences University of Melbourne Victoria AustraliaSchool of Biosciences University of Melbourne Victoria AustraliaAbstract Premise Seaweeds are gaining substantial research interest, particularly for genomic applications, where high‐quality DNA is a prerequisite. Extracting DNA from these organisms presents challenges due to high levels of biomacromolecules resulting from their diverse cell structures. Existing protocols often lack versatility, leading to inconsistent outcomes across various materials and taxa, which highlights the need for a universal method for use with a variety of green seaweed samples. Methods and Results We optimized the conventional cetyltrimethylammonium bromide (CTAB) protocol for green seaweed DNA extraction. Our method, involving an initial sample treatment, lysis buffer adjustment, and enzyme incubation alterations, outperformed the conventional CTAB and commercial kits in terms of DNA yield and purity. Notably, the protocol's effectiveness was demonstrated across various green algal materials and preservation methods, and was tested with downstream applications with satisfactory results. Conclusions Our optimized CTAB protocol offers a reliable solution for high‐quality genomic DNA extraction from a wide variety of green seaweed samples.https://doi.org/10.1002/aps3.11625absorbance ratioshigh‐molecular‐weight DNAmacrophytemarineseaweed |
| spellingShingle | Riyad Hossen Myles Courtney Alasdair Sim Md Abdullah Al Kamran Khan Heroen Verbruggen Trevor Bringloe An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) Applications in Plant Sciences absorbance ratios high‐molecular‐weight DNA macrophyte marine seaweed |
| title | An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) |
| title_full | An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) |
| title_fullStr | An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) |
| title_full_unstemmed | An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) |
| title_short | An optimized CTAB method for genomic DNA extraction from green seaweeds (Ulvophyceae) |
| title_sort | optimized ctab method for genomic dna extraction from green seaweeds ulvophyceae |
| topic | absorbance ratios high‐molecular‐weight DNA macrophyte marine seaweed |
| url | https://doi.org/10.1002/aps3.11625 |
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