The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses
Background. The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. Methods. We evaluated t...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2017/1078062 |
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| author | Malene Nerstrøm Peter-Martin Krarup Lars Nannestad Jorgensen Magnus S. Ågren |
| author_facet | Malene Nerstrøm Peter-Martin Krarup Lars Nannestad Jorgensen Magnus S. Ågren |
| author_sort | Malene Nerstrøm |
| collection | DOAJ |
| description | Background. The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. Methods. We evaluated the following two treatment groups of male Sprague Dawley rats (220–256 g): control-treated colonic anastomoses (n=25) and OTR4120-treated colonic anastomoses (n=25). We resected 10 mm of the left colon and then applied either saline alone (control) or OTR4120 (100 μg/mL) in saline to the colonic ends before an end-to-end single-layer anastomosis was constructed and again on the anastomosis before the abdomen and skin were closed. Results. On postoperative day 3, the anastomotic breaking strengths were 1.47 ± 0.32 N (mean ± SD) in the control group and 1.52 ± 0.27 N in the OTR4120-treated animals (P=0.622). We also found that the hydroxyproline concentration (indicator of collagen) in the anastomotic wounds did not differ (P=0.571) between the two groups. Conclusions. Our data demonstrate that a single local application of OTR4120 intraoperatively did not increase the biomechanical strength of colonic anastomoses at the critical postoperative day 3 when the anastomoses are the weakest. |
| format | Article |
| id | doaj-art-0a700f9857e74a159090a1a05222382f |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | Gastroenterology Research and Practice |
| spelling | doaj-art-0a700f9857e74a159090a1a05222382f2025-02-03T01:09:47ZengWileyGastroenterology Research and Practice1687-61211687-630X2017-01-01201710.1155/2017/10780621078062The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic AnastomosesMalene Nerstrøm0Peter-Martin Krarup1Lars Nannestad Jorgensen2Magnus S. Ågren3Digestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkDigestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkDigestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkDigestive Disease Center, Bispebjerg Hospital, University of Copenhagen, Bispebjerg Bakke 23, 2400 Copenhagen NV, DenmarkBackground. The mimetic compound OTR4120 may replace endogenous-degraded heparan sulfates that normally maintain the bioactivity of growth factors that are important for tissue repair. Herein, we investigated the effect of OTR4120 on the healing of normal colonic anastomoses. Methods. We evaluated the following two treatment groups of male Sprague Dawley rats (220–256 g): control-treated colonic anastomoses (n=25) and OTR4120-treated colonic anastomoses (n=25). We resected 10 mm of the left colon and then applied either saline alone (control) or OTR4120 (100 μg/mL) in saline to the colonic ends before an end-to-end single-layer anastomosis was constructed and again on the anastomosis before the abdomen and skin were closed. Results. On postoperative day 3, the anastomotic breaking strengths were 1.47 ± 0.32 N (mean ± SD) in the control group and 1.52 ± 0.27 N in the OTR4120-treated animals (P=0.622). We also found that the hydroxyproline concentration (indicator of collagen) in the anastomotic wounds did not differ (P=0.571) between the two groups. Conclusions. Our data demonstrate that a single local application of OTR4120 intraoperatively did not increase the biomechanical strength of colonic anastomoses at the critical postoperative day 3 when the anastomoses are the weakest.http://dx.doi.org/10.1155/2017/1078062 |
| spellingShingle | Malene Nerstrøm Peter-Martin Krarup Lars Nannestad Jorgensen Magnus S. Ågren The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses Gastroenterology Research and Practice |
| title | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
| title_full | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
| title_fullStr | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
| title_full_unstemmed | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
| title_short | The Effect of a Synthetic Heparan Sulfate on the Healing of Colonic Anastomoses |
| title_sort | effect of a synthetic heparan sulfate on the healing of colonic anastomoses |
| url | http://dx.doi.org/10.1155/2017/1078062 |
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