The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study
Iron is needed for life-essential processes, but free iron overload causes dangerous clinical consequences. The study of the role of red blood cells (RBCs) in the influence of excess free iron in the blood on the pathological consequences in an organism is relevant. Here, in a direct biophysical exp...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2022/7113958 |
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| author | E. Kozlova E. Sherstyukova V. Sergunova A. Kozlov O. Gudkova V. Inozemtsev A. Chernysh |
| author_facet | E. Kozlova E. Sherstyukova V. Sergunova A. Kozlov O. Gudkova V. Inozemtsev A. Chernysh |
| author_sort | E. Kozlova |
| collection | DOAJ |
| description | Iron is needed for life-essential processes, but free iron overload causes dangerous clinical consequences. The study of the role of red blood cells (RBCs) in the influence of excess free iron in the blood on the pathological consequences in an organism is relevant. Here, in a direct biophysical experiment in vitro, we studied the action of free iron overload on the packed red blood cell (pRBC) characteristics. In experiments, we incubated pRBCs with the ferrous sulfate solution (Fe2+). Wе used free iron in a wide range of concentrations. High Fe2+ concentrations made us possible to establish the pattern of the toxic effect of excess iron on pRBCs during a reduced incubation time in a biophysical experiment in vitro. It was found that excess free iron causes changes in pRBC morphology, the appearance of bridges between cells, and the formation of clots, increasing the membrane stiffness and methemoglobin concentration. We created a kinetic model of changes in the hemoglobin derivatives. The complex of simultaneous distortions of pRBCs established in our experiments can be taken into account when studying the mechanism of the toxic influence of excess free iron in the blood on pathological changes in an organism. |
| format | Article |
| id | doaj-art-0a68d20c13f944b68c850b5f7c0e47a2 |
| institution | Kabale University |
| issn | 1687-8205 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-0a68d20c13f944b68c850b5f7c0e47a22025-02-03T01:02:29ZengWileyJournal of Toxicology1687-82052022-01-01202210.1155/2022/7113958The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro StudyE. Kozlova0E. Sherstyukova1V. Sergunova2A. Kozlov3O. Gudkova4V. Inozemtsev5A. Chernysh6Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyFederal Research and Clinical Center of Intensive Care Medicine and RehabilitologyFederal Research and Clinical Center of Intensive Care Medicine and RehabilitologySechenov First Moscow State Medical University (Sechenov University)Federal Research and Clinical Center of Intensive Care Medicine and RehabilitologyFederal Research and Clinical Center of Intensive Care Medicine and RehabilitologyFederal Research and Clinical Center of Intensive Care Medicine and RehabilitologyIron is needed for life-essential processes, but free iron overload causes dangerous clinical consequences. The study of the role of red blood cells (RBCs) in the influence of excess free iron in the blood on the pathological consequences in an organism is relevant. Here, in a direct biophysical experiment in vitro, we studied the action of free iron overload on the packed red blood cell (pRBC) characteristics. In experiments, we incubated pRBCs with the ferrous sulfate solution (Fe2+). Wе used free iron in a wide range of concentrations. High Fe2+ concentrations made us possible to establish the pattern of the toxic effect of excess iron on pRBCs during a reduced incubation time in a biophysical experiment in vitro. It was found that excess free iron causes changes in pRBC morphology, the appearance of bridges between cells, and the formation of clots, increasing the membrane stiffness and methemoglobin concentration. We created a kinetic model of changes in the hemoglobin derivatives. The complex of simultaneous distortions of pRBCs established in our experiments can be taken into account when studying the mechanism of the toxic influence of excess free iron in the blood on pathological changes in an organism.http://dx.doi.org/10.1155/2022/7113958 |
| spellingShingle | E. Kozlova E. Sherstyukova V. Sergunova A. Kozlov O. Gudkova V. Inozemtsev A. Chernysh The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study Journal of Toxicology |
| title | The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study |
| title_full | The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study |
| title_fullStr | The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study |
| title_full_unstemmed | The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study |
| title_short | The Toxic Influence of Excess Free Iron on Red Blood Cells in the Biophysical Experiment: An In Vitro Study |
| title_sort | toxic influence of excess free iron on red blood cells in the biophysical experiment an in vitro study |
| url | http://dx.doi.org/10.1155/2022/7113958 |
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