Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization
Organic fertilizer application promotes the prevalence of antibiotic resistance genes (ARGs), yet the factors driving temporal differences in ARG abundance under long-term organic fertilizer application remain unclear. This study investigated the temporal dynamics of ARG diversity and abundance in b...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412024007669 |
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| author | Xiujing Guan Yuhui Li Yanying Yang Zihua Liu Rongguang Shi Yan Xu |
| author_facet | Xiujing Guan Yuhui Li Yanying Yang Zihua Liu Rongguang Shi Yan Xu |
| author_sort | Xiujing Guan |
| collection | DOAJ |
| description | Organic fertilizer application promotes the prevalence of antibiotic resistance genes (ARGs), yet the factors driving temporal differences in ARG abundance under long-term organic fertilizer application remain unclear. This study investigated the temporal dynamics of ARG diversity and abundance in both bulk and rhizosphere soils over 17 years (2003–2019), and explored microbial evolution strategies, ARG hosts succession and the influence of root exudates on ARGs regulation. The results showed that the ARGs abundance in rhizosphere soil was lower than that in bulk soil under long-term fertilization, and ARGs abundance exhibited a decrease and then remained stable in rhizosphere soil over time. There was a strong association between host bacteria and dominant ARGs (p < 0.05). Structural equations demonstrated that bacterial community had a most pronounced influence on ARGs (p < 0.05), and metabolites exhibited an important mediation effect on bacterial community (p < 0.05), thereby impacting ARGs. The metabolome analysis evidenced that significant correlations were found between defensive root exudates and most ARGs abundance (p < 0.05), like, luteolin-7-glucoside was negatively correlated with tetA(58). These findings provide deeper insights into the dynamics of soil ARGs under long-term fertilization, and identify critical factors that influence ARGs colonization in soils, providing support for controlling the spread of ARGs in agriculture soils. |
| format | Article |
| id | doaj-art-0a43f78908c44ecbb1c461854040cd30 |
| institution | Kabale University |
| issn | 0160-4120 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Environment International |
| spelling | doaj-art-0a43f78908c44ecbb1c461854040cd302025-01-24T04:43:59ZengElsevierEnvironment International0160-41202025-01-01195109180Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilizationXiujing Guan0Yuhui Li1Yanying Yang2Zihua Liu3Rongguang Shi4Yan Xu5Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaAgro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaCorresponding authors.; Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaCorresponding authors.; Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, ChinaOrganic fertilizer application promotes the prevalence of antibiotic resistance genes (ARGs), yet the factors driving temporal differences in ARG abundance under long-term organic fertilizer application remain unclear. This study investigated the temporal dynamics of ARG diversity and abundance in both bulk and rhizosphere soils over 17 years (2003–2019), and explored microbial evolution strategies, ARG hosts succession and the influence of root exudates on ARGs regulation. The results showed that the ARGs abundance in rhizosphere soil was lower than that in bulk soil under long-term fertilization, and ARGs abundance exhibited a decrease and then remained stable in rhizosphere soil over time. There was a strong association between host bacteria and dominant ARGs (p < 0.05). Structural equations demonstrated that bacterial community had a most pronounced influence on ARGs (p < 0.05), and metabolites exhibited an important mediation effect on bacterial community (p < 0.05), thereby impacting ARGs. The metabolome analysis evidenced that significant correlations were found between defensive root exudates and most ARGs abundance (p < 0.05), like, luteolin-7-glucoside was negatively correlated with tetA(58). These findings provide deeper insights into the dynamics of soil ARGs under long-term fertilization, and identify critical factors that influence ARGs colonization in soils, providing support for controlling the spread of ARGs in agriculture soils.http://www.sciencedirect.com/science/article/pii/S0160412024007669FertilizationAntibiotic resistance genesRhizosphereMetabolome analysisMicrobial evolution strategies |
| spellingShingle | Xiujing Guan Yuhui Li Yanying Yang Zihua Liu Rongguang Shi Yan Xu Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization Environment International Fertilization Antibiotic resistance genes Rhizosphere Metabolome analysis Microbial evolution strategies |
| title | Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization |
| title_full | Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization |
| title_fullStr | Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization |
| title_full_unstemmed | Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization |
| title_short | Root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long-term fertilization |
| title_sort | root exudates regulate soil antibiotic resistance genes via rhizosphere microbes under long term fertilization |
| topic | Fertilization Antibiotic resistance genes Rhizosphere Metabolome analysis Microbial evolution strategies |
| url | http://www.sciencedirect.com/science/article/pii/S0160412024007669 |
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