Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice
BackgroundAlthough diarrhea is a preventable disease, it continues to have a significant impact on global health, with the burden being much greater in Sub-Saharan Africa. Medicinal plants represent affordable and locally available resources to address many diseases, including diarrhea. Thus, this s...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1510171/full |
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| author | Khalid Ibrahim Kassaw Dawit Zewdu Wondafrash Jibril Seid Yesuf Mestayet Geta Mengistie |
| author_facet | Khalid Ibrahim Kassaw Dawit Zewdu Wondafrash Jibril Seid Yesuf Mestayet Geta Mengistie |
| author_sort | Khalid Ibrahim Kassaw |
| collection | DOAJ |
| description | BackgroundAlthough diarrhea is a preventable disease, it continues to have a significant impact on global health, with the burden being much greater in Sub-Saharan Africa. Medicinal plants represent affordable and locally available resources to address many diseases, including diarrhea. Thus, this study aimed to investigate the antidiarrheal activities of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice.MethodsThis study was carried out by administering the 80% hydromethanolic crude extract and solvent fractions from the crude extract, including the n-hexane fraction (NHF), ethyl acetate fraction (EAF), and aqueous fraction (AF) at doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively, to the mice. The effects of these extracts and solvent fractions on reducing/delaying diarrhea were compared with the impact of the solvent used for reconstitution and a standard drug (loperamide 3 mg/kg or atropine 5 mg/kg), as well as with each other.ResultsThe hydromethanolic crude extract and ethyl acetate fraction at all tested doses significantly reduced wet defecation (P < 0.05). In addition, total defecation was significantly reduced at a dose of 200 mg/kg (P < 0.05) and 400 mg/kg (P < 0.001) of the crude extract and ethyl acetate fraction at all tested doses (P < 0.01. The aqueous fraction at 200 mg/kg and 400 mg/kg and the n-hexane fraction at 400 mg/kg (P < 0.05) significantly inhibit wet and total defecation. Likewise, in the enteropooling test, the crude extract and ethyl acetate fractions showed a significant reduction in the weight and volume of intestinal contents at a dose of 200 mg/kg (P < 0.05) and 400 mg/kg (P < 0.05). Castor oil-induced intestinal motility was significantly reduced (p < 0.001) by the crude extract and ethyl acetate fractions at all tested doses.ConclusionThe result from this study suggests significant antidiarrheal activity of Terminalia brownii leaves, which validates its traditional use. The 80% hydromethanolic crude extract and ethyl acetate solvent fractions of Terminalia brownii Fresen leaves have shown better antidiarrheal activity. |
| format | Article |
| id | doaj-art-0a38916fa4f54c898caf867c01b5b396 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Pharmacology |
| spelling | doaj-art-0a38916fa4f54c898caf867c01b5b3962025-01-27T06:41:05ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15101711510171Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino miceKhalid Ibrahim Kassaw0Dawit Zewdu Wondafrash1Jibril Seid Yesuf2Mestayet Geta Mengistie3Department of Biomedical Science, School of Medicine, College of Medical and Health Sciences, Samara University, Semera, EthiopiaDepartment of Pharmacology, School of Medicine, Saint Paul’s Hospital Millennium Medical College, Addis Ababa, EthiopiaDepartment of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, EthiopiaDepartment of Pharmacology, College of Medicine and Health Sciences, University of Gondar, Gondar, EthiopiaBackgroundAlthough diarrhea is a preventable disease, it continues to have a significant impact on global health, with the burden being much greater in Sub-Saharan Africa. Medicinal plants represent affordable and locally available resources to address many diseases, including diarrhea. Thus, this study aimed to investigate the antidiarrheal activities of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice.MethodsThis study was carried out by administering the 80% hydromethanolic crude extract and solvent fractions from the crude extract, including the n-hexane fraction (NHF), ethyl acetate fraction (EAF), and aqueous fraction (AF) at doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg, respectively, to the mice. The effects of these extracts and solvent fractions on reducing/delaying diarrhea were compared with the impact of the solvent used for reconstitution and a standard drug (loperamide 3 mg/kg or atropine 5 mg/kg), as well as with each other.ResultsThe hydromethanolic crude extract and ethyl acetate fraction at all tested doses significantly reduced wet defecation (P < 0.05). In addition, total defecation was significantly reduced at a dose of 200 mg/kg (P < 0.05) and 400 mg/kg (P < 0.001) of the crude extract and ethyl acetate fraction at all tested doses (P < 0.01. The aqueous fraction at 200 mg/kg and 400 mg/kg and the n-hexane fraction at 400 mg/kg (P < 0.05) significantly inhibit wet and total defecation. Likewise, in the enteropooling test, the crude extract and ethyl acetate fractions showed a significant reduction in the weight and volume of intestinal contents at a dose of 200 mg/kg (P < 0.05) and 400 mg/kg (P < 0.05). Castor oil-induced intestinal motility was significantly reduced (p < 0.001) by the crude extract and ethyl acetate fractions at all tested doses.ConclusionThe result from this study suggests significant antidiarrheal activity of Terminalia brownii leaves, which validates its traditional use. The 80% hydromethanolic crude extract and ethyl acetate solvent fractions of Terminalia brownii Fresen leaves have shown better antidiarrheal activity.https://www.frontiersin.org/articles/10.3389/fphar.2024.1510171/fullantidiarrheal activityTerminalia brownii FresenCombretaceaehydromethanolic crude extractcastor oil-induced diarrhea |
| spellingShingle | Khalid Ibrahim Kassaw Dawit Zewdu Wondafrash Jibril Seid Yesuf Mestayet Geta Mengistie Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice Frontiers in Pharmacology antidiarrheal activity Terminalia brownii Fresen Combretaceae hydromethanolic crude extract castor oil-induced diarrhea |
| title | Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice |
| title_full | Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice |
| title_fullStr | Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice |
| title_full_unstemmed | Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice |
| title_short | Evaluation of the antidiarrheal activity of the 80% hydromethanolic crude extract and solvent fractions of Terminalia brownii Fresen (Combretaceae) leaves in Swiss Albino mice |
| title_sort | evaluation of the antidiarrheal activity of the 80 hydromethanolic crude extract and solvent fractions of terminalia brownii fresen combretaceae leaves in swiss albino mice |
| topic | antidiarrheal activity Terminalia brownii Fresen Combretaceae hydromethanolic crude extract castor oil-induced diarrhea |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1510171/full |
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