Electrochemical Glucose Sensor Based on Dual Redox Mediators
Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and R...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/9 |
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| author | Changyun Quan Yue Zhang Yuanyuan Liu Liping Wen Haixia Yang Xueqin Huang Minghui Yang Binjie Xu |
| author_facet | Changyun Quan Yue Zhang Yuanyuan Liu Liping Wen Haixia Yang Xueqin Huang Minghui Yang Binjie Xu |
| author_sort | Changyun Quan |
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| description | Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. Experimental results indicated the synergy between PD and Ru(III) provided a promising electron transfer environment for a glucose dehydrogenase (GDH)-catalyzed glucose reaction. The sensor exhibits a linear glucose response range from 0.01 to 38.6 mmol/L, with a limit of detection (LOD) as low as 7.0 µmol/L and a sensitivity of 38 µA·L/(mmol·cm<sup>2</sup>). The accuracy of the sensor was further validated in spiked recovery tests of human venous blood samples. The glucose recovery rate was between 99.5% and 107%, with a relative standard deviation (RSD) of less than 3.2%. These results demonstrate that our sensor has high potential for commercialization and practical application in glucose monitoring. |
| format | Article |
| id | doaj-art-0a2386721cca48e88202c85d5afac410 |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-0a2386721cca48e88202c85d5afac4102025-01-24T13:25:25ZengMDPI AGBiosensors2079-63742024-12-01151910.3390/bios15010009Electrochemical Glucose Sensor Based on Dual Redox MediatorsChangyun Quan0Yue Zhang1Yuanyuan Liu2Liping Wen3Haixia Yang4Xueqin Huang5Minghui Yang6Binjie Xu7Hunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, ChinaHunan Institute For Drug Control, Changsha 410001, ChinaCofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, ChinaCofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, ChinaCofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, ChinaHunan Provincial Key Laboratory of Micro & Nano Materials Interface Science, College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, ChinaCofoe Medical Technology Co., Ltd., No. 816 Zhenghua Road, Changsha 410021, ChinaElectrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. Experimental results indicated the synergy between PD and Ru(III) provided a promising electron transfer environment for a glucose dehydrogenase (GDH)-catalyzed glucose reaction. The sensor exhibits a linear glucose response range from 0.01 to 38.6 mmol/L, with a limit of detection (LOD) as low as 7.0 µmol/L and a sensitivity of 38 µA·L/(mmol·cm<sup>2</sup>). The accuracy of the sensor was further validated in spiked recovery tests of human venous blood samples. The glucose recovery rate was between 99.5% and 107%, with a relative standard deviation (RSD) of less than 3.2%. These results demonstrate that our sensor has high potential for commercialization and practical application in glucose monitoring.https://www.mdpi.com/2079-6374/15/1/9electrochemical glucose sensordual redox mediatorHexaammineruthenium trichloride1,10-Phenanthroline-5,6-dione |
| spellingShingle | Changyun Quan Yue Zhang Yuanyuan Liu Liping Wen Haixia Yang Xueqin Huang Minghui Yang Binjie Xu Electrochemical Glucose Sensor Based on Dual Redox Mediators Biosensors electrochemical glucose sensor dual redox mediator Hexaammineruthenium trichloride 1,10-Phenanthroline-5,6-dione |
| title | Electrochemical Glucose Sensor Based on Dual Redox Mediators |
| title_full | Electrochemical Glucose Sensor Based on Dual Redox Mediators |
| title_fullStr | Electrochemical Glucose Sensor Based on Dual Redox Mediators |
| title_full_unstemmed | Electrochemical Glucose Sensor Based on Dual Redox Mediators |
| title_short | Electrochemical Glucose Sensor Based on Dual Redox Mediators |
| title_sort | electrochemical glucose sensor based on dual redox mediators |
| topic | electrochemical glucose sensor dual redox mediator Hexaammineruthenium trichloride 1,10-Phenanthroline-5,6-dione |
| url | https://www.mdpi.com/2079-6374/15/1/9 |
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