Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt
Introduction: Pseudomonas aeruginosa is one of the most virulent nosocomial pathogens worldwide. Quorum sensing (QS) regulates the production of pathogenic virulence factors and biofilm formation in P. aeruginosa. The four genes lasR, lasI, rhlR,and rhlI were found to regulate this QS system. In thi...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Journal of Infection in Developing Countries
2015-10-01
|
| Series: | Journal of Infection in Developing Countries |
| Subjects: | |
| Online Access: | https://jidc.org/index.php/journal/article/view/6492 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850193345493598208 |
|---|---|
| author | Hamida M. Aboushleib Hoda M. Omar Rania Abozahra Amel Elsheredy Kholoud Baraka |
| author_facet | Hamida M. Aboushleib Hoda M. Omar Rania Abozahra Amel Elsheredy Kholoud Baraka |
| author_sort | Hamida M. Aboushleib |
| collection | DOAJ |
| description | Introduction: Pseudomonas aeruginosa is one of the most virulent nosocomial pathogens worldwide. Quorum sensing (QS) regulates the production of pathogenic virulence factors and biofilm formation in P. aeruginosa. The four genes lasR, lasI, rhlR,and rhlI were found to regulate this QS system. In this study, we aimed to assess the correlation between these four genes and QS-dependent virulence factors and to detect the inhibitory effect of clove oil on QS.
Methodology: Fifty P. aeruginosa clinical isolates were collected. Susceptibility to different antibiotics was tested. Virulence factors including biofilm formation, pyocyanin production, and twitching motility were phenotypically detected. QS genes were amplified by polymerase chain reaction (PCR), and one strain subsequently underwent sequencing. The inhibitory effect of clove oil on virulence factors was also tested.
Results: A positive correlation was found between biofilm formation and the presence of lasR and rhlI genes. Twitching motility was positively correlated with the presence of lasR, lasI, and rhlI genes. On the other hand, no correlation was found between pyocyanin production and any of the studied genes. Only one isolate amplified all the tested QS gene primers, but it did not express any of the tested virulence factors phenotypically. Sequence analyses of this isolate showed that the four genes had point mutations.
Conclusions: Results emphasize the importance of QS in P. aeruginosa virulence; however, QS-deficient clinical isolates occur and are still capable of causing clinical infections in humans. Also, clove oil has an obvious inhibitory effect on QS, which should be clinically exploited.
|
| format | Article |
| id | doaj-art-0a0f07bcdbd044e4a99e57f0eb50d2f1 |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-0a0f07bcdbd044e4a99e57f0eb50d2f12025-08-20T02:14:19ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-10-0191010.3855/jidc.6492Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in EgyptHamida M. Aboushleib0Hoda M. Omar1Rania Abozahra2Amel Elsheredy3Kholoud Baraka4Faculty of Pharmacy, Alexandria University, Alexandria, EgyptFaculty of Pharmacy, Alexandria University, Alexandria, EgyptFaculty of Pharmacy, Damanhour University, Damanhour, EgyptMedical Research Institute, Alexandria University, Alexandria, EgyptFaculty of Pharmacy, Damanhour University, Damanhour, EgyptIntroduction: Pseudomonas aeruginosa is one of the most virulent nosocomial pathogens worldwide. Quorum sensing (QS) regulates the production of pathogenic virulence factors and biofilm formation in P. aeruginosa. The four genes lasR, lasI, rhlR,and rhlI were found to regulate this QS system. In this study, we aimed to assess the correlation between these four genes and QS-dependent virulence factors and to detect the inhibitory effect of clove oil on QS. Methodology: Fifty P. aeruginosa clinical isolates were collected. Susceptibility to different antibiotics was tested. Virulence factors including biofilm formation, pyocyanin production, and twitching motility were phenotypically detected. QS genes were amplified by polymerase chain reaction (PCR), and one strain subsequently underwent sequencing. The inhibitory effect of clove oil on virulence factors was also tested. Results: A positive correlation was found between biofilm formation and the presence of lasR and rhlI genes. Twitching motility was positively correlated with the presence of lasR, lasI, and rhlI genes. On the other hand, no correlation was found between pyocyanin production and any of the studied genes. Only one isolate amplified all the tested QS gene primers, but it did not express any of the tested virulence factors phenotypically. Sequence analyses of this isolate showed that the four genes had point mutations. Conclusions: Results emphasize the importance of QS in P. aeruginosa virulence; however, QS-deficient clinical isolates occur and are still capable of causing clinical infections in humans. Also, clove oil has an obvious inhibitory effect on QS, which should be clinically exploited. https://jidc.org/index.php/journal/article/view/6492quorum sensingvirulencePseudomonas aeruginosagenesbiofilm |
| spellingShingle | Hamida M. Aboushleib Hoda M. Omar Rania Abozahra Amel Elsheredy Kholoud Baraka Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt Journal of Infection in Developing Countries quorum sensing virulence Pseudomonas aeruginosa genes biofilm |
| title | Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt |
| title_full | Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt |
| title_fullStr | Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt |
| title_full_unstemmed | Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt |
| title_short | Correlation of quorum sensing and virulence factors in Pseudomonas aeruginosa isolates in Egypt |
| title_sort | correlation of quorum sensing and virulence factors in pseudomonas aeruginosa isolates in egypt |
| topic | quorum sensing virulence Pseudomonas aeruginosa genes biofilm |
| url | https://jidc.org/index.php/journal/article/view/6492 |
| work_keys_str_mv | AT hamidamaboushleib correlationofquorumsensingandvirulencefactorsinpseudomonasaeruginosaisolatesinegypt AT hodamomar correlationofquorumsensingandvirulencefactorsinpseudomonasaeruginosaisolatesinegypt AT raniaabozahra correlationofquorumsensingandvirulencefactorsinpseudomonasaeruginosaisolatesinegypt AT amelelsheredy correlationofquorumsensingandvirulencefactorsinpseudomonasaeruginosaisolatesinegypt AT kholoudbaraka correlationofquorumsensingandvirulencefactorsinpseudomonasaeruginosaisolatesinegypt |