MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain
In recent years, the wine industry has been researching how to improve wine quality along the production value chain. In this scenario, we present here a new tool, MicroVi, a cost-effective chip-sized microscopy solution to detect and count yeast cells in wine samples. We demonstrate that this novel...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/40 |
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| author | Ismael Benito-Altamirano Sergio Moreno David M. Vaz-Romero Anna Puig-Pujol Gemma Roca-Domènech Joan Canals Anna Vilà Joan Daniel Prades Ángel Diéguez |
| author_facet | Ismael Benito-Altamirano Sergio Moreno David M. Vaz-Romero Anna Puig-Pujol Gemma Roca-Domènech Joan Canals Anna Vilà Joan Daniel Prades Ángel Diéguez |
| author_sort | Ismael Benito-Altamirano |
| collection | DOAJ |
| description | In recent years, the wine industry has been researching how to improve wine quality along the production value chain. In this scenario, we present here a new tool, MicroVi, a cost-effective chip-sized microscopy solution to detect and count yeast cells in wine samples. We demonstrate that this novel microscopy setup is able to measure the same type of samples as an optical microscopy system, but with smaller size equipment and with automated cell count configuration. The technology relies on the top of state-of-the-art computer vision pipelines to post-process the images and count the cells. A typical pipeline consists of normalization, feature extraction (i.e., SIFT), image composition (to increase both resolution and scanning area), holographic reconstruction and particle count (i.e., Hough transform). MicroVi achieved a 2.19 µm resolution by properly resolving the G7.6 features from the USAF Resolving Power Test Target 1951. Additionally, we aimed for a successful calibration of cell counts for <i>Saccharomyces cerevisiae</i>. We compared our direct results with our current optical setup, achieving a linear calibration for measurements ranging from 0.5 to 50 million cells per milliliter. Furthermore, other yeast cells were qualitatively resolved with our MicroVi microscope, such as, <i>Brettanomyces bruxellensis</i>, or bacteria, like, <i>Lactobacillus plantarum</i>, thus confirming the system’s reliability for consistent microbial assessment. |
| format | Article |
| id | doaj-art-0a0d8e30082d4f0d91636c0f8d35191a |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-0a0d8e30082d4f0d91636c0f8d35191a2025-01-24T13:25:31ZengMDPI AGBiosensors2079-63742025-01-011514010.3390/bios15010040MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value ChainIsmael Benito-Altamirano0Sergio Moreno1David M. Vaz-Romero2Anna Puig-Pujol3Gemma Roca-Domènech4Joan Canals5Anna Vilà6Joan Daniel Prades7Ángel Diéguez8Department of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainINCAVI-IRTA, Catalan Institute of Vine and Wine—Institute of Agrifood Research and Technology, Plaça Àgora, 2, Vilafranca del Penedès, 08720 Barcelona, SpainINCAVI-IRTA, Catalan Institute of Vine and Wine—Institute of Agrifood Research and Technology, Plaça Àgora, 2, Vilafranca del Penedès, 08720 Barcelona, SpainDepartment of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainDepartment of Electronic and Biomedical Engineering, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, SpainIn recent years, the wine industry has been researching how to improve wine quality along the production value chain. In this scenario, we present here a new tool, MicroVi, a cost-effective chip-sized microscopy solution to detect and count yeast cells in wine samples. We demonstrate that this novel microscopy setup is able to measure the same type of samples as an optical microscopy system, but with smaller size equipment and with automated cell count configuration. The technology relies on the top of state-of-the-art computer vision pipelines to post-process the images and count the cells. A typical pipeline consists of normalization, feature extraction (i.e., SIFT), image composition (to increase both resolution and scanning area), holographic reconstruction and particle count (i.e., Hough transform). MicroVi achieved a 2.19 µm resolution by properly resolving the G7.6 features from the USAF Resolving Power Test Target 1951. Additionally, we aimed for a successful calibration of cell counts for <i>Saccharomyces cerevisiae</i>. We compared our direct results with our current optical setup, achieving a linear calibration for measurements ranging from 0.5 to 50 million cells per milliliter. Furthermore, other yeast cells were qualitatively resolved with our MicroVi microscope, such as, <i>Brettanomyces bruxellensis</i>, or bacteria, like, <i>Lactobacillus plantarum</i>, thus confirming the system’s reliability for consistent microbial assessment.https://www.mdpi.com/2079-6374/15/1/40ab-on-a-chipyeast cell countholographywine qualitychip-sized microscopy |
| spellingShingle | Ismael Benito-Altamirano Sergio Moreno David M. Vaz-Romero Anna Puig-Pujol Gemma Roca-Domènech Joan Canals Anna Vilà Joan Daniel Prades Ángel Diéguez MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain Biosensors ab-on-a-chip yeast cell count holography wine quality chip-sized microscopy |
| title | MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain |
| title_full | MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain |
| title_fullStr | MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain |
| title_full_unstemmed | MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain |
| title_short | MicroVi: A Cost-Effective Microscopy Solution for Yeast Cell Detection and Count in Wine Value Chain |
| title_sort | microvi a cost effective microscopy solution for yeast cell detection and count in wine value chain |
| topic | ab-on-a-chip yeast cell count holography wine quality chip-sized microscopy |
| url | https://www.mdpi.com/2079-6374/15/1/40 |
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