Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles
Infectious diseases caused by antibiotic-resistant bacteria lead to a considerable increase in human morbidity and mortality globally. This requires to search potential actinomycete isolates from undiscovered habitats as a source of effective bioactive metabolites and to synthesis metabolite-mediate...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/8816111 |
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| author | Abebe Bizuye Lashitew Gedamu Christine Bii Erastus Gatebe Naomi Maina |
| author_facet | Abebe Bizuye Lashitew Gedamu Christine Bii Erastus Gatebe Naomi Maina |
| author_sort | Abebe Bizuye |
| collection | DOAJ |
| description | Infectious diseases caused by antibiotic-resistant bacteria lead to a considerable increase in human morbidity and mortality globally. This requires to search potential actinomycete isolates from undiscovered habitats as a source of effective bioactive metabolites and to synthesis metabolite-mediated antibacterial silver nanoparticles (AgNPs). The main purpose of the present study was to identify actinomycetes isolated from Thika waste dump soils that produce bioactive metabolites to synthesize antibacterial AgNPs. The synthesis of metabolite-mediated AgNP was confirmed with visual detection and a UV-vis spectrophotometer, whereas the functional groups involved in AgNP synthesis were identified using a FTIR spectrophotometer. The antibacterial activity of the metabolite-mediated AgNPs was tested by a well diffusion assay. Identification of actinomycete isolates involved in the synthesis of antibacterial AgNPs was done based on 16S rRNA gene sequence analysis. The visual detection showed that dark salmon and pale golden color change was observed due to the formation of AgNPs by KDT32 and KGT32 metabolites, respectively. The synthesis was confirmed by a characteristic UV spectra peak at 415.5 nm for KDT32-AgNP and 416 nm for KGT32-AgNP. The FTIR spectra revealed that OH, C=C, and S-S functional groups were involved in the synthesis of KDT32-AgNP, whereas OH, C=C, and C-H were involved in the formation of KGT32-AgNP. The inhibition zone results revealed that KDT32-AgNP showed 22.0 ± 1.4 mm and 19.0 ± 1.4 mm against Escherichia coli and Salmonella typhi, whereas KGT32-AgNP showed 21.5 ± 0.7 mm and 17.0 ± 0.0 mm, respectively. KDT32 and KGT32 isolates were identified as genus Streptomyces and their 16S rRNA gene sequences were deposited in the GenBank database with MH301089 and MH301090 accession numbers, respectively. Due to the bactericidal activity of synthesized AgNPs, KDT32 and KGT32 isolates can be used in biomedical applications. |
| format | Article |
| id | doaj-art-0a0a027e76c14312b660f31ffa45e8ec |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-0a0a027e76c14312b660f31ffa45e8ec2025-02-03T06:43:41ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/88161118816111Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver NanoparticlesAbebe Bizuye0Lashitew Gedamu1Christine Bii2Erastus Gatebe3Naomi Maina4Department of Medical Laboratory, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, EthiopiaDepartment of Biological Sciences, University of Calgary, Calgary, CanadaCentre for Microbiology Research, Kenya Medical Research Institute, Nairobi, KenyaKenya Industrial Research Development and Innovation, Nairobi, KenyaMolecular Biology and Biotechnology, Pan African University Institute of Basic Sciences, Innovation and Technology, Jomo Kenyatta University of Agriculture and Technology, Nairobi, KenyaInfectious diseases caused by antibiotic-resistant bacteria lead to a considerable increase in human morbidity and mortality globally. This requires to search potential actinomycete isolates from undiscovered habitats as a source of effective bioactive metabolites and to synthesis metabolite-mediated antibacterial silver nanoparticles (AgNPs). The main purpose of the present study was to identify actinomycetes isolated from Thika waste dump soils that produce bioactive metabolites to synthesize antibacterial AgNPs. The synthesis of metabolite-mediated AgNP was confirmed with visual detection and a UV-vis spectrophotometer, whereas the functional groups involved in AgNP synthesis were identified using a FTIR spectrophotometer. The antibacterial activity of the metabolite-mediated AgNPs was tested by a well diffusion assay. Identification of actinomycete isolates involved in the synthesis of antibacterial AgNPs was done based on 16S rRNA gene sequence analysis. The visual detection showed that dark salmon and pale golden color change was observed due to the formation of AgNPs by KDT32 and KGT32 metabolites, respectively. The synthesis was confirmed by a characteristic UV spectra peak at 415.5 nm for KDT32-AgNP and 416 nm for KGT32-AgNP. The FTIR spectra revealed that OH, C=C, and S-S functional groups were involved in the synthesis of KDT32-AgNP, whereas OH, C=C, and C-H were involved in the formation of KGT32-AgNP. The inhibition zone results revealed that KDT32-AgNP showed 22.0 ± 1.4 mm and 19.0 ± 1.4 mm against Escherichia coli and Salmonella typhi, whereas KGT32-AgNP showed 21.5 ± 0.7 mm and 17.0 ± 0.0 mm, respectively. KDT32 and KGT32 isolates were identified as genus Streptomyces and their 16S rRNA gene sequences were deposited in the GenBank database with MH301089 and MH301090 accession numbers, respectively. Due to the bactericidal activity of synthesized AgNPs, KDT32 and KGT32 isolates can be used in biomedical applications.http://dx.doi.org/10.1155/2020/8816111 |
| spellingShingle | Abebe Bizuye Lashitew Gedamu Christine Bii Erastus Gatebe Naomi Maina Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles International Journal of Microbiology |
| title | Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles |
| title_full | Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles |
| title_fullStr | Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles |
| title_full_unstemmed | Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles |
| title_short | Molecular-Based Identification of Actinomycetes Species That Synthesize Antibacterial Silver Nanoparticles |
| title_sort | molecular based identification of actinomycetes species that synthesize antibacterial silver nanoparticles |
| url | http://dx.doi.org/10.1155/2020/8816111 |
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