A Heating and Cooling Stage With Fast Temporal Control for Biological Applications
The study of biological processes involving live microscopy techniques requires adequate temperature control to respect the physiology of the organism under study. We present here a design strategy for a microscope temperature stage based on thermoelectric elements. The design allows the user to acc...
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| Format: | Article |
| Language: | English |
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IEEE
2024-01-01
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| Series: | IEEE Open Journal of Engineering in Medicine and Biology |
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| Online Access: | https://ieeexplore.ieee.org/document/10596548/ |
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| author | Manon Valet Juan M. Iglesias-Artola Falk Elsner Anatol W. Fritsch Otger Campas |
| author_facet | Manon Valet Juan M. Iglesias-Artola Falk Elsner Anatol W. Fritsch Otger Campas |
| author_sort | Manon Valet |
| collection | DOAJ |
| description | The study of biological processes involving live microscopy techniques requires adequate temperature control to respect the physiology of the organism under study. We present here a design strategy for a microscope temperature stage based on thermoelectric elements. The design allows the user to access a range of temperatures below and above room temperature and can accommodate samples of different geometries. In addition, by cooling simultaneously the sample insert and the objective, we minimize the temperature gradients along the sample for large magnification objectives requiring immersion oil. We illustrate how this design can be used to study the physiology of the zebrafish embryo over the temperature tolerance of this species. We envision that this device could benefit the communities using model and non-model organisms with physiological temperatures different from typical mammalian cell culture incubation in biomedical research. |
| format | Article |
| id | doaj-art-09f8681a5836477fa411fb68d391d74c |
| institution | Kabale University |
| issn | 2644-1276 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | IEEE |
| record_format | Article |
| series | IEEE Open Journal of Engineering in Medicine and Biology |
| spelling | doaj-art-09f8681a5836477fa411fb68d391d74c2025-01-30T00:03:54ZengIEEEIEEE Open Journal of Engineering in Medicine and Biology2644-12762024-01-01557357510.1109/OJEMB.2024.342691210596548A Heating and Cooling Stage With Fast Temporal Control for Biological ApplicationsManon Valet0https://orcid.org/0000-0002-0051-0663Juan M. Iglesias-Artola1https://orcid.org/0000-0002-0409-795XFalk Elsner2Anatol W. Fritsch3https://orcid.org/0000-0003-0986-7068Otger Campas4https://orcid.org/0000-0002-9642-8030Cluster of Excellence Physics of Life, TU Dresden, Dresden, GermanyMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, GermanyMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, GermanyMax Planck Institute of Molecular Cell Biology and Genetics, Dresden, GermanyCluster of Excellence Physics of Life, TU Dresden, Dresden, GermanyThe study of biological processes involving live microscopy techniques requires adequate temperature control to respect the physiology of the organism under study. We present here a design strategy for a microscope temperature stage based on thermoelectric elements. The design allows the user to access a range of temperatures below and above room temperature and can accommodate samples of different geometries. In addition, by cooling simultaneously the sample insert and the objective, we minimize the temperature gradients along the sample for large magnification objectives requiring immersion oil. We illustrate how this design can be used to study the physiology of the zebrafish embryo over the temperature tolerance of this species. We envision that this device could benefit the communities using model and non-model organisms with physiological temperatures different from typical mammalian cell culture incubation in biomedical research.https://ieeexplore.ieee.org/document/10596548/Live microscopypoikilothermytemperature controlthermal cardiac tolerancezebrafish |
| spellingShingle | Manon Valet Juan M. Iglesias-Artola Falk Elsner Anatol W. Fritsch Otger Campas A Heating and Cooling Stage With Fast Temporal Control for Biological Applications IEEE Open Journal of Engineering in Medicine and Biology Live microscopy poikilothermy temperature control thermal cardiac tolerance zebrafish |
| title | A Heating and Cooling Stage With Fast Temporal Control for Biological Applications |
| title_full | A Heating and Cooling Stage With Fast Temporal Control for Biological Applications |
| title_fullStr | A Heating and Cooling Stage With Fast Temporal Control for Biological Applications |
| title_full_unstemmed | A Heating and Cooling Stage With Fast Temporal Control for Biological Applications |
| title_short | A Heating and Cooling Stage With Fast Temporal Control for Biological Applications |
| title_sort | heating and cooling stage with fast temporal control for biological applications |
| topic | Live microscopy poikilothermy temperature control thermal cardiac tolerance zebrafish |
| url | https://ieeexplore.ieee.org/document/10596548/ |
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