Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest
The main purpose of the present study was to evaluate the antitumor effects of pinocembrin in human prostate cancer cells (PC-3) along with investigating its effects on cell apoptosis, endogenous ROS production and cell cycle. MTT assay and clonogenic assays were used to study the effects on cell vi...
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| Language: | English |
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Sciendo
2021-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2021-0042 |
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| author | Shao Linhai Shao Yajun Yuan Yu |
| author_facet | Shao Linhai Shao Yajun Yuan Yu |
| author_sort | Shao Linhai |
| collection | DOAJ |
| description | The main purpose of the present study was to evaluate the antitumor effects of pinocembrin in human prostate cancer cells (PC-3) along with investigating its effects on cell apoptosis, endogenous ROS production and cell cycle. MTT assay and clonogenic assays were used to study the effects on cell viability and cancer colony formation, respectively. Fluorescence microscopy along with Western blotting was used to study apoptotic effects induced by pinocembrin. Flow cytometry was used to study effects on ROS production and cell cycle phase distribution. Results indicated that pinocembrin promoted inhibition cell proliferation along with reducing cancer colony formation of PC-3 cells in a dose-dependent manner. Pinocembrin induced regulatory effects over expressions of caspase-3, caspase-9, Bax and Bcl-2, thereby promoting apoptotic cell death in PC-3 cells. It also led to the dose-dependent G0/G1 cell cycle arrest. In conclusion, pinocembrin exhibits strong anticancer effects in human prostate cancer cells mediated via apoptosis, endogenous ROS production and G0/G1 cell cycle arrest. |
| format | Article |
| id | doaj-art-09f420f181134cf9b259059302a2df7d |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-09f420f181134cf9b259059302a2df7d2025-02-02T10:03:15ZengSciendoActa Pharmaceutica1846-95582021-12-0171466967810.2478/acph-2021-0042Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrestShao Linhai0Shao Yajun1Yuan Yu2Department of Urology, Hanzhong Central Hospital, Shaanxi Province Hanzhong, 723000, ChinaDepartment of Ultrasound, Xi’an NO.1 Hospital, Shaanxi Province, Xi’an 710002, ChinaDepartment of Ophthalmology, Hanzhong Central Hospital, Shaanxi Province, Hanzhong, 723000, ChinaThe main purpose of the present study was to evaluate the antitumor effects of pinocembrin in human prostate cancer cells (PC-3) along with investigating its effects on cell apoptosis, endogenous ROS production and cell cycle. MTT assay and clonogenic assays were used to study the effects on cell viability and cancer colony formation, respectively. Fluorescence microscopy along with Western blotting was used to study apoptotic effects induced by pinocembrin. Flow cytometry was used to study effects on ROS production and cell cycle phase distribution. Results indicated that pinocembrin promoted inhibition cell proliferation along with reducing cancer colony formation of PC-3 cells in a dose-dependent manner. Pinocembrin induced regulatory effects over expressions of caspase-3, caspase-9, Bax and Bcl-2, thereby promoting apoptotic cell death in PC-3 cells. It also led to the dose-dependent G0/G1 cell cycle arrest. In conclusion, pinocembrin exhibits strong anticancer effects in human prostate cancer cells mediated via apoptosis, endogenous ROS production and G0/G1 cell cycle arrest.https://doi.org/10.2478/acph-2021-0042prostate cancerflavonespinocembrinapoptosiscell cycle arrest |
| spellingShingle | Shao Linhai Shao Yajun Yuan Yu Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest Acta Pharmaceutica prostate cancer flavones pinocembrin apoptosis cell cycle arrest |
| title | Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest |
| title_full | Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest |
| title_fullStr | Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest |
| title_full_unstemmed | Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest |
| title_short | Pinocembrin flavanone inhibits cell viability in PC-3 human prostate cancer by inducing cellular apoptosis, ROS production and cell cycle arrest |
| title_sort | pinocembrin flavanone inhibits cell viability in pc 3 human prostate cancer by inducing cellular apoptosis ros production and cell cycle arrest |
| topic | prostate cancer flavones pinocembrin apoptosis cell cycle arrest |
| url | https://doi.org/10.2478/acph-2021-0042 |
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