Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks
This study aimed to investigate the effects of a diet supplemented with different levels of oleic acid (OA) on growth performance, serum biochemical parameters, nutrient utilization, and intestinal lipid metabolism in Pekin ducks. A total of 350 fourteen-d-old male ducks were randomly assigned to th...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-05-01
|
| Series: | Poultry Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125002743 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850136951927078912 |
|---|---|
| author | W.X. Zhang G. Tian K.Y. Zhang S.P. Bai X.M. Ding J.P. Wang Y. Xuan Q.F. Zeng |
| author_facet | W.X. Zhang G. Tian K.Y. Zhang S.P. Bai X.M. Ding J.P. Wang Y. Xuan Q.F. Zeng |
| author_sort | W.X. Zhang |
| collection | DOAJ |
| description | This study aimed to investigate the effects of a diet supplemented with different levels of oleic acid (OA) on growth performance, serum biochemical parameters, nutrient utilization, and intestinal lipid metabolism in Pekin ducks. A total of 350 fourteen-d-old male ducks were randomly assigned to the following five isonitrogenous and heteroenergetic dietary treatment groups: 0.00% (control), 0.25%, 0.50%, 0.75%, and 1.00% OA groups. The experiment lasted 28 days. The findings indicated that neither growth performance nor nutrient utilization was affected by OA supplementation (P > 0.05). The 0.50% OA group displayed the lowest serum triglyceride (TG) levels among all treatment groups, with significantly lower values compared to both the 0.25%=% and 0.75% OA groups (P < 0.05). Moreover, the activities of lipid droplet (LD)-degrading enzymes in the jejunal mucosa, such as adipose triglyceride lipase (ATGL), showed a significant inverse linear relationship (P < 0.05); carboxylesterase 2 (CES2) activity exhibited a proportional dose-dependent increase (P < 0.05); and lysosomal acid lipase (LAL) activity was negatively correlated with the increased concentration of OA in the diet (P < 0.05). Moreover, the mRNA expression levels of the LD formation-related genes PLIN2 were significantly higher in the 0.50% OA group compared to the 0.25% and 0.75% OA groups (P < 0.05). The mRNA expression of LD degradation-related genes, the PNPLA2 expression in the 0.25%, 0.50%, and 0.75% OA groups and LPL expression in all OA groups were downregulated (P < 0.05) when compared with those in the control group. These results suggested that dietary supplementation with OA, especially at a level of 0.50%, may decrease the serum TG content and promote lipid deposition in the jejunum in Pekin ducks by regulating the formation and degradation of enterocyte LDs. |
| format | Article |
| id | doaj-art-09ca8ad9900a4db5b3ba8a4b227c5dd1 |
| institution | OA Journals |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-09ca8ad9900a4db5b3ba8a4b227c5dd12025-08-20T02:30:59ZengElsevierPoultry Science0032-57912025-05-01104510503510.1016/j.psj.2025.105035Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducksW.X. Zhang0G. Tian1K.Y. Zhang2S.P. Bai3X.M. Ding4J.P. Wang5Y. Xuan6Q.F. Zeng7Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR ChinaInstitute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan, PR China; Key Laboratory for Animal Disease-Resistance Nutrition, Ministry of Education, Ministry of Agriculture and Rural Affairs, Sichuan Province 611130, PR China; Corresponding author.This study aimed to investigate the effects of a diet supplemented with different levels of oleic acid (OA) on growth performance, serum biochemical parameters, nutrient utilization, and intestinal lipid metabolism in Pekin ducks. A total of 350 fourteen-d-old male ducks were randomly assigned to the following five isonitrogenous and heteroenergetic dietary treatment groups: 0.00% (control), 0.25%, 0.50%, 0.75%, and 1.00% OA groups. The experiment lasted 28 days. The findings indicated that neither growth performance nor nutrient utilization was affected by OA supplementation (P > 0.05). The 0.50% OA group displayed the lowest serum triglyceride (TG) levels among all treatment groups, with significantly lower values compared to both the 0.25%=% and 0.75% OA groups (P < 0.05). Moreover, the activities of lipid droplet (LD)-degrading enzymes in the jejunal mucosa, such as adipose triglyceride lipase (ATGL), showed a significant inverse linear relationship (P < 0.05); carboxylesterase 2 (CES2) activity exhibited a proportional dose-dependent increase (P < 0.05); and lysosomal acid lipase (LAL) activity was negatively correlated with the increased concentration of OA in the diet (P < 0.05). Moreover, the mRNA expression levels of the LD formation-related genes PLIN2 were significantly higher in the 0.50% OA group compared to the 0.25% and 0.75% OA groups (P < 0.05). The mRNA expression of LD degradation-related genes, the PNPLA2 expression in the 0.25%, 0.50%, and 0.75% OA groups and LPL expression in all OA groups were downregulated (P < 0.05) when compared with those in the control group. These results suggested that dietary supplementation with OA, especially at a level of 0.50%, may decrease the serum TG content and promote lipid deposition in the jejunum in Pekin ducks by regulating the formation and degradation of enterocyte LDs.http://www.sciencedirect.com/science/article/pii/S0032579125002743Oleic acidSerum triglycerideEnterocyte lipid dropletsPekin ducks |
| spellingShingle | W.X. Zhang G. Tian K.Y. Zhang S.P. Bai X.M. Ding J.P. Wang Y. Xuan Q.F. Zeng Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks Poultry Science Oleic acid Serum triglyceride Enterocyte lipid droplets Pekin ducks |
| title | Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks |
| title_full | Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks |
| title_fullStr | Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks |
| title_full_unstemmed | Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks |
| title_short | Effects of dietary supplementation with oleic acid on growth performance, dietary fat utilization, serum and intestinal lipid metabolic parameters, and enterocyte lipid droplet metabolism in Pekin ducks |
| title_sort | effects of dietary supplementation with oleic acid on growth performance dietary fat utilization serum and intestinal lipid metabolic parameters and enterocyte lipid droplet metabolism in pekin ducks |
| topic | Oleic acid Serum triglyceride Enterocyte lipid droplets Pekin ducks |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125002743 |
| work_keys_str_mv | AT wxzhang effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT gtian effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT kyzhang effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT spbai effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT xmding effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT jpwang effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT yxuan effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks AT qfzeng effectsofdietarysupplementationwitholeicacidongrowthperformancedietaryfatutilizationserumandintestinallipidmetabolicparametersandenterocytelipiddropletmetabolisminpekinducks |