Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India
Abstract Aedes aegypti and Aedes albopictus mosquitoes spread major vector-borne viral diseases in tropical and sub-tropical regions of the globe. In this study, we sequenced the genome of Indian Ae. aegypti and Ae. albopictus and mapped to their reference genomes. Comparative genomics were performe...
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Nature Portfolio
2024-10-01
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| author | Preeti Acharya Upasana Shyamsunder Singh Veeramanoharan Rajamannar Mayilsamy Muniaraj Binata Nayak Aparup Das |
| author_facet | Preeti Acharya Upasana Shyamsunder Singh Veeramanoharan Rajamannar Mayilsamy Muniaraj Binata Nayak Aparup Das |
| author_sort | Preeti Acharya |
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| description | Abstract Aedes aegypti and Aedes albopictus mosquitoes spread major vector-borne viral diseases in tropical and sub-tropical regions of the globe. In this study, we sequenced the genome of Indian Ae. aegypti and Ae. albopictus and mapped to their reference genomes. Comparative genomics were performed between our strain and the reference strains. A total of 14,416,484 single nucleotide polymorphisms (SNPs) and 156,487 insertions and deletions (InDels) were found in Ae. aegypti, and 28,940,433 SNPs and 188,987 InDels in Ae. albopictus. Particular emphasis was given to gene families involved in mosquito digestion, development, and innate immunity, which could be putative candidates for vector control. Serine protease cascades and their inhibitors called serpins, play a central role in these processes. We extracted high-impact variants in genes associated with serine proteases and serpins. This study reports for the first time a high coverage genome sequence data of an Indian Ae. albopictus mosquito. The results from this study will provide insights into Indian Aedes specific polymorphisms and the evolution of immune related genes in mosquitoes, which can serve as a resource for future comparative genomics and those pursuing the development of targeted biopesticides for effective mosquito control strategies. |
| format | Article |
| id | doaj-art-09c68e923fca451bae470cad4ee2598c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-09c68e923fca451bae470cad4ee2598c2025-02-02T12:25:19ZengNature PortfolioScientific Reports2045-23222024-10-0114111010.1038/s41598-024-71484-2Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south IndiaPreeti Acharya0Upasana Shyamsunder Singh1Veeramanoharan Rajamannar2Mayilsamy Muniaraj3Binata Nayak4Aparup Das5Sambalpur UniversityDepartment of Biological Sciences, Vanderbilt UniversityICMR-Vector Control Research Centre Field StationICMR-Vector Control Research Centre Field StationSambalpur UniversityICMR-National Institute of Research in Tribal HealthAbstract Aedes aegypti and Aedes albopictus mosquitoes spread major vector-borne viral diseases in tropical and sub-tropical regions of the globe. In this study, we sequenced the genome of Indian Ae. aegypti and Ae. albopictus and mapped to their reference genomes. Comparative genomics were performed between our strain and the reference strains. A total of 14,416,484 single nucleotide polymorphisms (SNPs) and 156,487 insertions and deletions (InDels) were found in Ae. aegypti, and 28,940,433 SNPs and 188,987 InDels in Ae. albopictus. Particular emphasis was given to gene families involved in mosquito digestion, development, and innate immunity, which could be putative candidates for vector control. Serine protease cascades and their inhibitors called serpins, play a central role in these processes. We extracted high-impact variants in genes associated with serine proteases and serpins. This study reports for the first time a high coverage genome sequence data of an Indian Ae. albopictus mosquito. The results from this study will provide insights into Indian Aedes specific polymorphisms and the evolution of immune related genes in mosquitoes, which can serve as a resource for future comparative genomics and those pursuing the development of targeted biopesticides for effective mosquito control strategies.https://doi.org/10.1038/s41598-024-71484-2GenomeAedesSerine proteaseSerpinDNA polymorphismDengue |
| spellingShingle | Preeti Acharya Upasana Shyamsunder Singh Veeramanoharan Rajamannar Mayilsamy Muniaraj Binata Nayak Aparup Das Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India Scientific Reports Genome Aedes Serine protease Serpin DNA polymorphism Dengue |
| title | Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India |
| title_full | Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India |
| title_fullStr | Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India |
| title_full_unstemmed | Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India |
| title_short | Genome resequencing and genome-wide polymorphisms in mosquito vectors Aedes aegypti and Aedes albopictus from south India |
| title_sort | genome resequencing and genome wide polymorphisms in mosquito vectors aedes aegypti and aedes albopictus from south india |
| topic | Genome Aedes Serine protease Serpin DNA polymorphism Dengue |
| url | https://doi.org/10.1038/s41598-024-71484-2 |
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