Suppression of Leukotriene B4 Generation by Ex-vivo Neutrophils Isolated from Asthma Patients on Dietary Supplementation with Gammalinolenic Acid-containing Borage Oil: Possible Implication in Asthma
Dietary gammalinolenic acid (GLA), a potent inhibitor of 5-lipoxygenase (5-LOX) and suppressor of leukotriene B4 (LTB4), can attenuate the clinical course of rheumatoid arthritics, with negligible side effects. Since Zileuton, also an inhibitor of 5-LOX, attenuates asthma but with an undesirable sid...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1080/10446670410001670445 |
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| Summary: | Dietary gammalinolenic acid (GLA), a potent inhibitor of 5-lipoxygenase (5-LOX) and suppressor of
leukotriene B4 (LTB4), can attenuate the clinical course of rheumatoid arthritics, with negligible side
effects. Since Zileuton, also an inhibitor of 5-LOX, attenuates asthma but with an undesirable side
effect, we investigated whether dietary GLA would suppress biosynthesis of PMN-LTB4 isolated from
asthma patients and attenuate asthma. Twenty-four mild-moderate asthma patients (16–75 years) were
randomized to receive either 2.0 g daily GLA (borage oil) or corn oil (placebo) for 12 months. Blood
drawn at 3 months intervals was used to prepare sera for fatty acid analysis, PMNs for determining
phospholipid fatty acids and for LTB4 generation. Patients were monitored by daily asthma scores,
pulmonary function, and exhaled NO. Ingestion of daily GLA (i) increased DGLA (GLA metabolite) in
PMN-phospholipids; (ii) increased generation of PMN-15-HETrE (5-LOX metabolite of DGLA).
Increased PMN-DGLA/15-HETrE paralleled the decreased PMN generation of proinflammatory LTB4.
However, the suppression of PMN-LTB4 did not reveal statistically significant suppression of the
asthma scores evaluated. Nonetheless, the study demonstrated dietary fatty acid modulation of
endogenous inflammatory mediators without side effects and thus warrant further explorations into the
roles of GLA at higher doses, leukotrienes and asthma. |
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| ISSN: | 1740-2522 1740-2530 |