Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss
The aim of this research was to investigate the effects of Cucurbita pepo extract (CPE) on performance, blood parameters, immune responses, expression of immunity-related gene, and disease resistance in rainbow trout. The fish were fed with 0, 2, 4, and 6 % of CPE in 15 tanks (100 L) for 8 weeks. Fi...
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| Language: | English |
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Elsevier
2025-06-01
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| Series: | Comparative Immunology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950311625000060 |
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| author | Kaveh Vakili Amir Eghbal Khajehrahimi Behnam Salimi Shapour Kakoolaki Arman Ghorbanzadeh |
| author_facet | Kaveh Vakili Amir Eghbal Khajehrahimi Behnam Salimi Shapour Kakoolaki Arman Ghorbanzadeh |
| author_sort | Kaveh Vakili |
| collection | DOAJ |
| description | The aim of this research was to investigate the effects of Cucurbita pepo extract (CPE) on performance, blood parameters, immune responses, expression of immunity-related gene, and disease resistance in rainbow trout. The fish were fed with 0, 2, 4, and 6 % of CPE in 15 tanks (100 L) for 8 weeks. Fish fed with 4 % of CPE showed significantly higher values of growth indices compared to control diet (P < 0.05). The highest hematocrit and hemoglobin contents were associated with the diet containing 4 % CPE. Similarly, dietary 4 % of CPE effectively increased lysozyme activity, and immunoglobulin content compared to the control diet. Furthermore, the highest mRNA level of tumor necrosis factor (TNF-α) was observed in 4 % CPE diet. Following the bacterial challenge, the highest relative percentage survival value was observed in fish fed with 4 % CPE. The current study demonstrated dietary incorporation of 4 % CPE can improve performance, hemato-immunological parameters and resistance of rainbow trout against Y. ruckeri infection. |
| format | Article |
| id | doaj-art-0920e11fd4444372a41319018da03370 |
| institution | Kabale University |
| issn | 2950-3116 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Comparative Immunology Reports |
| spelling | doaj-art-0920e11fd4444372a41319018da033702025-01-26T05:05:31ZengElsevierComparative Immunology Reports2950-31162025-06-018200200Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykissKaveh Vakili0Amir Eghbal Khajehrahimi1Behnam Salimi2Shapour Kakoolaki3Arman Ghorbanzadeh4Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, Iran; Corresponding author.Department of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Aquatic Animal Health and Disease, Sanandaj Branch, Islamic Azad University, Sanandaj, IranDepartment of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, IranDepartment of Food Hygiene, Science and Research Branch, Islamic Azad University, Tehran, IranThe aim of this research was to investigate the effects of Cucurbita pepo extract (CPE) on performance, blood parameters, immune responses, expression of immunity-related gene, and disease resistance in rainbow trout. The fish were fed with 0, 2, 4, and 6 % of CPE in 15 tanks (100 L) for 8 weeks. Fish fed with 4 % of CPE showed significantly higher values of growth indices compared to control diet (P < 0.05). The highest hematocrit and hemoglobin contents were associated with the diet containing 4 % CPE. Similarly, dietary 4 % of CPE effectively increased lysozyme activity, and immunoglobulin content compared to the control diet. Furthermore, the highest mRNA level of tumor necrosis factor (TNF-α) was observed in 4 % CPE diet. Following the bacterial challenge, the highest relative percentage survival value was observed in fish fed with 4 % CPE. The current study demonstrated dietary incorporation of 4 % CPE can improve performance, hemato-immunological parameters and resistance of rainbow trout against Y. ruckeri infection.http://www.sciencedirect.com/science/article/pii/S2950311625000060Herbal extractGrowthImmunityGene expressionRainbow troutDisease resistance |
| spellingShingle | Kaveh Vakili Amir Eghbal Khajehrahimi Behnam Salimi Shapour Kakoolaki Arman Ghorbanzadeh Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss Comparative Immunology Reports Herbal extract Growth Immunity Gene expression Rainbow trout Disease resistance |
| title | Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss |
| title_full | Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss |
| title_fullStr | Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss |
| title_full_unstemmed | Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss |
| title_short | Effect of dietary Cucurbita pepo extract on performance, hemato-immunological parameters, immune-related gene expression and bacterial resistance in rainbow trout, Oncorhynchus mykiss |
| title_sort | effect of dietary cucurbita pepo extract on performance hemato immunological parameters immune related gene expression and bacterial resistance in rainbow trout oncorhynchus mykiss |
| topic | Herbal extract Growth Immunity Gene expression Rainbow trout Disease resistance |
| url | http://www.sciencedirect.com/science/article/pii/S2950311625000060 |
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