Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings
Abstract The present study investigates the supplemental effects of chia seed oil (CSO) on the growth performance and modulation of intestinal microbiota in Labeo rohita fingerlings. Four diets were formulated with graded levels of CSO: 1.0%, 2.0%, and 3.0% represented as CSO (1), CSO (2), and, CSO...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83102-2 |
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| author | Sanjay Kumar Gupta Akruti Gupta Jaipal Singh Choudhary Md Javed Foysal Rajan Gupta Biplab Sarkar K. K. Krishnani |
| author_facet | Sanjay Kumar Gupta Akruti Gupta Jaipal Singh Choudhary Md Javed Foysal Rajan Gupta Biplab Sarkar K. K. Krishnani |
| author_sort | Sanjay Kumar Gupta |
| collection | DOAJ |
| description | Abstract The present study investigates the supplemental effects of chia seed oil (CSO) on the growth performance and modulation of intestinal microbiota in Labeo rohita fingerlings. Four diets were formulated with graded levels of CSO: 1.0%, 2.0%, and 3.0% represented as CSO (1), CSO (2), and, CSO (3) groups alongside a control group without CSO. L. rohita fingerlings (n = 180) (mean weight = 19.74 ± 0.33 g) were randomly distributed in triplicates for 60 days to these treatment groups. The results depicted significant improvements (p < 0.05) in weight gain (WG) %, specific growth rate (SGR), feed conversion ratio (FCR), and feed conversion efficiency (FCE) in the group supplemented with the lowest level of CSO. Gut microbial analysis evidenced the ability of CSO at 1.0% to augment the relative abundance of bacterial phyla such as Verrucomicrobiota, Actinobacteria, Bacteroidota, Fusobacteria and Firmicutes, as well as genera Luteolibacter and Cetobacterium, indicating higher alpha diversity compared to the control. Principle coordinate analysis (PCoA) demonstrated a distinct composition of microbial communities in CSO-supplemented groups relative to the control (p < 0.001). Correlation analysis further revealed a significant (p < 0.05) association of specific microbial taxa with growth performance parameters. The predictions of metabolic pathways suggested the involvement of carbohydrate and amino acid metabolic pathways in the CSO (1) group, indicating improved nutrient transport and metabolism. Overall, the findings highlight the beneficial effects of 1.0% CSO supplementation on growth performance and modulation of gut microbiota in L. rohita fingerlings. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-091f1d282f1f425d8d07428b664fbe222025-01-19T12:22:05ZengNature PortfolioScientific Reports2045-23222025-01-0115111810.1038/s41598-024-83102-2Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlingsSanjay Kumar Gupta0Akruti Gupta1Jaipal Singh Choudhary2Md Javed Foysal3Rajan Gupta4Biplab Sarkar5K. K. Krishnani6ICAR-Indian Institute of Agricultural BiotechnologyICAR-Indian Institute of Agricultural BiotechnologyICAR Research Complex for Eastern Region, Farming System Research Centre for Hill and Plateau RegionSchool of Molecular and Life Sciences, Curtin UniversityICAR-Indian Institute of Agricultural BiotechnologyICAR-Indian Institute of Agricultural BiotechnologyICAR-Indian Institute of Agricultural BiotechnologyAbstract The present study investigates the supplemental effects of chia seed oil (CSO) on the growth performance and modulation of intestinal microbiota in Labeo rohita fingerlings. Four diets were formulated with graded levels of CSO: 1.0%, 2.0%, and 3.0% represented as CSO (1), CSO (2), and, CSO (3) groups alongside a control group without CSO. L. rohita fingerlings (n = 180) (mean weight = 19.74 ± 0.33 g) were randomly distributed in triplicates for 60 days to these treatment groups. The results depicted significant improvements (p < 0.05) in weight gain (WG) %, specific growth rate (SGR), feed conversion ratio (FCR), and feed conversion efficiency (FCE) in the group supplemented with the lowest level of CSO. Gut microbial analysis evidenced the ability of CSO at 1.0% to augment the relative abundance of bacterial phyla such as Verrucomicrobiota, Actinobacteria, Bacteroidota, Fusobacteria and Firmicutes, as well as genera Luteolibacter and Cetobacterium, indicating higher alpha diversity compared to the control. Principle coordinate analysis (PCoA) demonstrated a distinct composition of microbial communities in CSO-supplemented groups relative to the control (p < 0.001). Correlation analysis further revealed a significant (p < 0.05) association of specific microbial taxa with growth performance parameters. The predictions of metabolic pathways suggested the involvement of carbohydrate and amino acid metabolic pathways in the CSO (1) group, indicating improved nutrient transport and metabolism. Overall, the findings highlight the beneficial effects of 1.0% CSO supplementation on growth performance and modulation of gut microbiota in L. rohita fingerlings.https://doi.org/10.1038/s41598-024-83102-2Chia seed oilgrowth performanceintestinal microbiota16S rRNA amplicon sequencingLabeo rohita |
| spellingShingle | Sanjay Kumar Gupta Akruti Gupta Jaipal Singh Choudhary Md Javed Foysal Rajan Gupta Biplab Sarkar K. K. Krishnani Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings Scientific Reports Chia seed oil growth performance intestinal microbiota 16S rRNA amplicon sequencing Labeo rohita |
| title | Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings |
| title_full | Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings |
| title_fullStr | Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings |
| title_full_unstemmed | Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings |
| title_short | Dietary Chia (Salvia hispanica L.) seeds oil supplementation augments growth performance and gut microbial composition in Labeo rohita fingerlings |
| title_sort | dietary chia salvia hispanica l seeds oil supplementation augments growth performance and gut microbial composition in labeo rohita fingerlings |
| topic | Chia seed oil growth performance intestinal microbiota 16S rRNA amplicon sequencing Labeo rohita |
| url | https://doi.org/10.1038/s41598-024-83102-2 |
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