Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii
The influence of sublethal concentrations of two sanitizers, liquid iodophor and liquid hypochlorite (LH), on the growth rates and toxicity of food-borne pathogenic Escherichia coli strains grown in the presence of spoilage yeast Zygosaccharomyces bailii was assessed. When grown in combination with...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/135856 |
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| author | Lerato Mogotsi Olga De Smidt Pierre Venter Willem Groenewald |
| author_facet | Lerato Mogotsi Olga De Smidt Pierre Venter Willem Groenewald |
| author_sort | Lerato Mogotsi |
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| description | The influence of sublethal concentrations of two sanitizers, liquid iodophor and liquid hypochlorite (LH), on the growth rates and toxicity of food-borne pathogenic Escherichia coli strains grown in the presence of spoilage yeast Zygosaccharomyces bailii was assessed. When grown in combination with Z. bailii both E. coli O113 and E. coli O26 exhibited slower growth rates, except when E. coli O113 was grown in combination with Z. bailii at 0.2% LH. The growth rate of Z. bailii was not impacted by the addition of the sanitizers or by communal growth with E. coli strains. LAL and IL-6 results indicated a decrease in toxicity of pure E. coli cultures with comparable profiles for control and sanitizer exposed samples, although the LAL assay proved to be more sensitive. Interestingly, pure cultures of Z. bailii showed increased toxicity measured by LAL and decreased toxicity measured by IL-6. LAL analysis showed a decrease in toxicity of both E. coli strains grown in combination with Z. bailii, while IL-6 analysis of the mixed cultures showed an increase in toxicity. The use of LAL for toxicity determination in a mixed culture overlooks the contribution made by spoilage yeast, thus demonstrating the importance of using the appropriate method for toxicity testing in mixed microbe environments. |
| format | Article |
| id | doaj-art-08f9ac65b6de46f89f2605b50908787c |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-08f9ac65b6de46f89f2605b50908787c2025-02-03T06:11:08ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/135856135856Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailiiLerato Mogotsi0Olga De Smidt1Pierre Venter2Willem Groenewald3Unit for Applied Food Science and Biotechnology, Central University of Technology, Free State, P.O. Box 20539, Bloemfontein 9300, South AfricaUnit for Applied Food Science and Biotechnology, Central University of Technology, Free State, P.O. Box 20539, Bloemfontein 9300, South AfricaFonterra Co-Operative Group Limited, Private Bag 11029, Palmerston North 4442, Dairy Farm Road 1, Palmerston North, New ZealandUnit for Applied Food Science and Biotechnology, Central University of Technology, Free State, P.O. Box 20539, Bloemfontein 9300, South AfricaThe influence of sublethal concentrations of two sanitizers, liquid iodophor and liquid hypochlorite (LH), on the growth rates and toxicity of food-borne pathogenic Escherichia coli strains grown in the presence of spoilage yeast Zygosaccharomyces bailii was assessed. When grown in combination with Z. bailii both E. coli O113 and E. coli O26 exhibited slower growth rates, except when E. coli O113 was grown in combination with Z. bailii at 0.2% LH. The growth rate of Z. bailii was not impacted by the addition of the sanitizers or by communal growth with E. coli strains. LAL and IL-6 results indicated a decrease in toxicity of pure E. coli cultures with comparable profiles for control and sanitizer exposed samples, although the LAL assay proved to be more sensitive. Interestingly, pure cultures of Z. bailii showed increased toxicity measured by LAL and decreased toxicity measured by IL-6. LAL analysis showed a decrease in toxicity of both E. coli strains grown in combination with Z. bailii, while IL-6 analysis of the mixed cultures showed an increase in toxicity. The use of LAL for toxicity determination in a mixed culture overlooks the contribution made by spoilage yeast, thus demonstrating the importance of using the appropriate method for toxicity testing in mixed microbe environments.http://dx.doi.org/10.1155/2014/135856 |
| spellingShingle | Lerato Mogotsi Olga De Smidt Pierre Venter Willem Groenewald Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii The Scientific World Journal |
| title | Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii |
| title_full | Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii |
| title_fullStr | Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii |
| title_full_unstemmed | Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii |
| title_short | Influence of Sanitizers on the Lipopolysaccharide Toxicity of Escherichia coli Strains Cultivated in the Presence of Zygosaccharomyces bailii |
| title_sort | influence of sanitizers on the lipopolysaccharide toxicity of escherichia coli strains cultivated in the presence of zygosaccharomyces bailii |
| url | http://dx.doi.org/10.1155/2014/135856 |
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