Screening and validation of tsRNAs associated with lung adenocarcinoma
Objective To explore the roles of transfer RNA-derived small RNAs （tsRNAs） in the oncogenesis and progression of lung adenocarcinoma by analyzing the differential expression of tsRNAs in lung adenocarcinoma and the relationship between the expression levels of tsRNAs in lung adenocarcinoma and the...
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Editorial Office of Journal of Army Medical University
2025-01-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | https://aammt.tmmu.edu.cn/html/202408059.html |
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| _version_ | 1832590722871590912 |
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| author | LU Chunli SHAN Yifan XIE Weijia |
| author_facet | LU Chunli SHAN Yifan XIE Weijia |
| author_sort | LU Chunli |
| collection | DOAJ |
| description | Objective To explore the roles of transfer RNA-derived small RNAs （tsRNAs） in the oncogenesis and progression of lung adenocarcinoma by analyzing the differential expression of tsRNAs in lung adenocarcinoma and the relationship between the expression levels of tsRNAs in lung adenocarcinoma and the prognosis of patients in order to further screen and validate the tsRNAs associated with lung adenocarcinoma. Methods The differential expression of tsRNAs between lung adenocarcinoma tissues and normal tissues was analyzed based on the database of the Computational Medicine Center. The effects of tsRNAs expression levels on the prognosis of lung adenocarcinoma patients were analyzed based on the Cancer Genome Atlas （TCGA） database （TCGA-LUAD）. The target genes were predicted based on TRFtarget2.0 and tRFTar databases. Gene ontology （GO） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis were performed based on DAVID and KOBA KEGG online websites. The expression levels of target genes in lung adenocarcinoma tissues and normal tissues were analyzed based on the University of ALabama at Birmingham CANcer data analysis Portal （UALCAN） database. In vitro cell proliferation， migration， and invasion assays were performed to investigate the biological functions of tRF-19-69M8LOJX in lung adenocarcinoma cells.Results Compared with the normal tissues， tRF-19-69M8LOJX was up-regulated in lung adenocarcinoma tissues （log2FC=4.28， FDR<0.05）. High expression level of tRF-19-69M8LOJX was associated with shorter progression-free survival （HR=1.565， 95%CI=1.142-2.145， P=0.005）. And its overexpression promoted cell proliferation and migration （P<0.001）， and invasion （P=0.009） of A549 cells， and up-regulated COL1A1 （P=0.002） and VCAN （P=0.022） significantly in the tRF-19-69M8LOJX overexpression cell model. Conclusion tRF-19-69M8LOJX is up-regulated in lung adenocarcinoma tissues. And its high expression is closely associated with poor prognosis. The tsRNA may play an important role in the pathogenesis and development of lung adenocarcinoma.
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| format | Article |
| id | doaj-art-08e10103a5b8492e8060196b89ba56fc |
| institution | Kabale University |
| issn | 2097-0927 |
| language | zho |
| publishDate | 2025-01-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj-art-08e10103a5b8492e8060196b89ba56fc2025-01-23T07:55:56ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272025-01-0147212213110.16016/j.2097-0927.202408059Screening and validation of tsRNAs associated with lung adenocarcinomaLU Chunli0SHAN Yifan1XIE Weijia2Department of Epidemiology， Faculty of Military Preventive Medicine， Army Medical University （Third Military Medical University）， Chongqing, PLA Key Laboratory of Respiratory Diseases， Second Affiliated Hospital， Army Medical University （Third Military Medical University）， Chongqing， ChinaDepartment of Epidemiology， Faculty of Military Preventive Medicine， Army Medical University （Third Military Medical University）， Chongqing, PLA Key Laboratory of Respiratory Diseases， Second Affiliated Hospital， Army Medical University （Third Military Medical University）， Chongqing， ChinaDepartment of Epidemiology， Faculty of Military Preventive Medicine， Army Medical University （Third Military Medical University）， Chongqing, PLA Key Laboratory of Respiratory Diseases， Second Affiliated Hospital， Army Medical University （Third Military Medical University）， Chongqing， ChinaObjective To explore the roles of transfer RNA-derived small RNAs （tsRNAs） in the oncogenesis and progression of lung adenocarcinoma by analyzing the differential expression of tsRNAs in lung adenocarcinoma and the relationship between the expression levels of tsRNAs in lung adenocarcinoma and the prognosis of patients in order to further screen and validate the tsRNAs associated with lung adenocarcinoma. Methods The differential expression of tsRNAs between lung adenocarcinoma tissues and normal tissues was analyzed based on the database of the Computational Medicine Center. The effects of tsRNAs expression levels on the prognosis of lung adenocarcinoma patients were analyzed based on the Cancer Genome Atlas （TCGA） database （TCGA-LUAD）. The target genes were predicted based on TRFtarget2.0 and tRFTar databases. Gene ontology （GO） enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analysis were performed based on DAVID and KOBA KEGG online websites. The expression levels of target genes in lung adenocarcinoma tissues and normal tissues were analyzed based on the University of ALabama at Birmingham CANcer data analysis Portal （UALCAN） database. In vitro cell proliferation， migration， and invasion assays were performed to investigate the biological functions of tRF-19-69M8LOJX in lung adenocarcinoma cells.Results Compared with the normal tissues， tRF-19-69M8LOJX was up-regulated in lung adenocarcinoma tissues （log2FC=4.28， FDR<0.05）. High expression level of tRF-19-69M8LOJX was associated with shorter progression-free survival （HR=1.565， 95%CI=1.142-2.145， P=0.005）. And its overexpression promoted cell proliferation and migration （P<0.001）， and invasion （P=0.009） of A549 cells， and up-regulated COL1A1 （P=0.002） and VCAN （P=0.022） significantly in the tRF-19-69M8LOJX overexpression cell model. Conclusion tRF-19-69M8LOJX is up-regulated in lung adenocarcinoma tissues. And its high expression is closely associated with poor prognosis. The tsRNA may play an important role in the pathogenesis and development of lung adenocarcinoma. https://aammt.tmmu.edu.cn/html/202408059.htmltransfer rna-derived small rnalung adenocarcinomaprognosis |
| spellingShingle | LU Chunli SHAN Yifan XIE Weijia Screening and validation of tsRNAs associated with lung adenocarcinoma 陆军军医大学学报 transfer rna-derived small rna lung adenocarcinoma prognosis |
| title | Screening and validation of tsRNAs associated with lung adenocarcinoma |
| title_full | Screening and validation of tsRNAs associated with lung adenocarcinoma |
| title_fullStr | Screening and validation of tsRNAs associated with lung adenocarcinoma |
| title_full_unstemmed | Screening and validation of tsRNAs associated with lung adenocarcinoma |
| title_short | Screening and validation of tsRNAs associated with lung adenocarcinoma |
| title_sort | screening and validation of tsrnas associated with lung adenocarcinoma |
| topic | transfer rna-derived small rna lung adenocarcinoma prognosis |
| url | https://aammt.tmmu.edu.cn/html/202408059.html |
| work_keys_str_mv | AT luchunli screeningandvalidationoftsrnasassociatedwithlungadenocarcinoma AT shanyifan screeningandvalidationoftsrnasassociatedwithlungadenocarcinoma AT xieweijia screeningandvalidationoftsrnasassociatedwithlungadenocarcinoma |