Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt
Abstract This study compared two Annona squamosa L. cultivars, Abdelrazik (Annona A.) and Balady (Annona B.), in terms of their chemical profile, in vitro cytotoxicity against HCT-116 and A549 cell lines, and total acetogenin. In addition, the two cultivars pulp were compared regarding carbohydrates...
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Nature Portfolio
2025-01-01
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| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-024-00368-6 |
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| author | Safaa Yassin Samah M. Elsohafy Amr El-Hawiet Maged S. Abdel-Kader Doaa A. Ghareeb Fikria A. Darwish Masouda E. Amer |
| author_facet | Safaa Yassin Samah M. Elsohafy Amr El-Hawiet Maged S. Abdel-Kader Doaa A. Ghareeb Fikria A. Darwish Masouda E. Amer |
| author_sort | Safaa Yassin |
| collection | DOAJ |
| description | Abstract This study compared two Annona squamosa L. cultivars, Abdelrazik (Annona A.) and Balady (Annona B.), in terms of their chemical profile, in vitro cytotoxicity against HCT-116 and A549 cell lines, and total acetogenin. In addition, the two cultivars pulp were compared regarding carbohydrates and magnesium ions content and immunomodulating activity. The two cultivars were also differentiated genetically by DNA barcoding using the universal primer matK and the specific primer Annona squamosa matK. The results showed that Annona A. seeds had higher acetogenin content and exhibited more potent cytotoxic activity against the two cell lines. In contrast, Annona B. pulp had higher carbohydrate content and lower magnesium ions content. The splenic lymphocyte proliferation assay revealed that Annona A. pulp extract was slightly more active as an immunostimulant. The specific primer used for DNA barcoding was more effective for species identification, while the universal primer was better for cultivar differentiation. Overall, our findings indicate the potential for using active compounds of Annona squamosa L. cultivars to develop new therapeutic agents for cancer therapy and immune enhancement. |
| format | Article |
| id | doaj-art-08712f3e43dd4355b1bce63238f6c22d |
| institution | Kabale University |
| issn | 2396-8370 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj-art-08712f3e43dd4355b1bce63238f6c22d2025-01-19T12:40:10ZengNature Portfolionpj Science of Food2396-83702025-01-01911910.1038/s41538-024-00368-6Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in EgyptSafaa Yassin0Samah M. Elsohafy1Amr El-Hawiet2Maged S. Abdel-Kader3Doaa A. Ghareeb4Fikria A. Darwish5Masouda E. Amer6Department of Pharmacognosy, Faculty of Pharmacy, Alexandria UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Alexandria UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Alexandria UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Alexandria UniversityDepartment of Biochemistry, Biological Screening and Preclinical Trial Laboratory, Faculty of Science, Alexandria UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Alexandria UniversityDepartment of Pharmacognosy, Faculty of Pharmacy, Alexandria UniversityAbstract This study compared two Annona squamosa L. cultivars, Abdelrazik (Annona A.) and Balady (Annona B.), in terms of their chemical profile, in vitro cytotoxicity against HCT-116 and A549 cell lines, and total acetogenin. In addition, the two cultivars pulp were compared regarding carbohydrates and magnesium ions content and immunomodulating activity. The two cultivars were also differentiated genetically by DNA barcoding using the universal primer matK and the specific primer Annona squamosa matK. The results showed that Annona A. seeds had higher acetogenin content and exhibited more potent cytotoxic activity against the two cell lines. In contrast, Annona B. pulp had higher carbohydrate content and lower magnesium ions content. The splenic lymphocyte proliferation assay revealed that Annona A. pulp extract was slightly more active as an immunostimulant. The specific primer used for DNA barcoding was more effective for species identification, while the universal primer was better for cultivar differentiation. Overall, our findings indicate the potential for using active compounds of Annona squamosa L. cultivars to develop new therapeutic agents for cancer therapy and immune enhancement.https://doi.org/10.1038/s41538-024-00368-6 |
| spellingShingle | Safaa Yassin Samah M. Elsohafy Amr El-Hawiet Maged S. Abdel-Kader Doaa A. Ghareeb Fikria A. Darwish Masouda E. Amer Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt npj Science of Food |
| title | Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt |
| title_full | Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt |
| title_fullStr | Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt |
| title_full_unstemmed | Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt |
| title_short | Comparative phytochemical and pharmacological analysis of two cultivars of Annona squamosa L. cultivated in Egypt |
| title_sort | comparative phytochemical and pharmacological analysis of two cultivars of annona squamosa l cultivated in egypt |
| url | https://doi.org/10.1038/s41538-024-00368-6 |
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