Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance
Protein feed resources in China are scarce, which hinders the developement of feed industry and animal husbandry. Yeast protein is rich in nutrients and has significant development potential. Yeast strains were isolated and identified under acidic conditions (pH 3.5), from fermented flour, bovine ru...
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Academy of National Food and Strategic Reserves Administration
2025-01-01
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| Series: | Liang you shipin ke-ji |
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| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250121 |
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| author | DENG Juan-li SUN Chang-po ZHAO Yi-fan LI Tian-tian YIN Peng |
| author_facet | DENG Juan-li SUN Chang-po ZHAO Yi-fan LI Tian-tian YIN Peng |
| author_sort | DENG Juan-li |
| collection | DOAJ |
| description | Protein feed resources in China are scarce, which hinders the developement of feed industry and animal husbandry. Yeast protein is rich in nutrients and has significant development potential. Yeast strains were isolated and identified under acidic conditions (pH 3.5), from fermented flour, bovine rumen chyme, corn steep liquor, and other raw materials. Through the determination of the growth performance, bacterial protein and flavor of the strains, a promising high-protein yeast strain was identified as a potential strain for yeast protein processing. Further, the research indicated that 20 acid-tolerant yeast strains, including Saccharomyces cerevisiae, Pichia fermentans, Kluyveromyces marxianus, Yarrowia lipolytica, Candida tropicalis, Starmerella bacillaris, Zygosaccharomyces rouxii and Hanseniaspora thailandica, were obtained from different raw materials. Two superior strains, Pichia pastoris B-1 (7.65 g/L biomass, 4.96 g/L protein yield at pH 3.5; 9.32 g/L biomass, 5.81 g/L protein yield at pH 7.0) and Lactobacillus cerevisiae D-1 (8.00 g/L biomass, 3.91 g/L protein yield at pH 3.5; 14.91 g/L biomass, 6.87 g/L protein yield at pH 7.0), were identified based on biomass and protein production under shaking flask culture conditions. Flavor analysis revealed that strain B-1 had a higher content of flavor nucleotides and glutamic acid than strain D-1, along with a greater concentration of ester compounds. Thus, strain B-1 was identified as the potential strain for further application.. Therefore, B-1 strain was determined as the target strain. Finally, the growth characteristics and stress tolerance of B-1 strain were examined. It exhibited robust growth under conditions of pH≥2.0, temperature≤40 ℃ and NaCl concentration ( w /v )≤70 g/L. |
| format | Article |
| id | doaj-art-08650063ec0042bf83b025a10bdc74cd |
| institution | Kabale University |
| issn | 1007-7561 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
| record_format | Article |
| series | Liang you shipin ke-ji |
| spelling | doaj-art-08650063ec0042bf83b025a10bdc74cd2025-01-23T14:28:51ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612025-01-0133119219910.16210/j.cnki.1007-7561.2025.01.020Screening and Characterization of High Protein Yeast with Excellent Stress ToleranceDENG Juan-li0SUN Chang-po1ZHAO Yi-fan2LI Tian-tian3YIN Peng4College of Food Science and Engineering, Henan University of Technology, Zhengzhou, Henan 450001, China; Institute of Cereal and Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaStandard Quality Center of National Food and Strategic Reserves Administration, Beijing 100834, ChinaInstitute of Cereal and Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Cereal and Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaInstitute of Cereal and Oil Science and Technology, Academy of National Food and Strategic Reserves Administration, Beijing 100037, ChinaProtein feed resources in China are scarce, which hinders the developement of feed industry and animal husbandry. Yeast protein is rich in nutrients and has significant development potential. Yeast strains were isolated and identified under acidic conditions (pH 3.5), from fermented flour, bovine rumen chyme, corn steep liquor, and other raw materials. Through the determination of the growth performance, bacterial protein and flavor of the strains, a promising high-protein yeast strain was identified as a potential strain for yeast protein processing. Further, the research indicated that 20 acid-tolerant yeast strains, including Saccharomyces cerevisiae, Pichia fermentans, Kluyveromyces marxianus, Yarrowia lipolytica, Candida tropicalis, Starmerella bacillaris, Zygosaccharomyces rouxii and Hanseniaspora thailandica, were obtained from different raw materials. Two superior strains, Pichia pastoris B-1 (7.65 g/L biomass, 4.96 g/L protein yield at pH 3.5; 9.32 g/L biomass, 5.81 g/L protein yield at pH 7.0) and Lactobacillus cerevisiae D-1 (8.00 g/L biomass, 3.91 g/L protein yield at pH 3.5; 14.91 g/L biomass, 6.87 g/L protein yield at pH 7.0), were identified based on biomass and protein production under shaking flask culture conditions. Flavor analysis revealed that strain B-1 had a higher content of flavor nucleotides and glutamic acid than strain D-1, along with a greater concentration of ester compounds. Thus, strain B-1 was identified as the potential strain for further application.. Therefore, B-1 strain was determined as the target strain. Finally, the growth characteristics and stress tolerance of B-1 strain were examined. It exhibited robust growth under conditions of pH≥2.0, temperature≤40 ℃ and NaCl concentration ( w /v )≤70 g/L.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250121high protein yeastscreeningflavorstress tolerance |
| spellingShingle | DENG Juan-li SUN Chang-po ZHAO Yi-fan LI Tian-tian YIN Peng Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance Liang you shipin ke-ji high protein yeast screening flavor stress tolerance |
| title | Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance |
| title_full | Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance |
| title_fullStr | Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance |
| title_full_unstemmed | Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance |
| title_short | Screening and Characterization of High Protein Yeast with Excellent Stress Tolerance |
| title_sort | screening and characterization of high protein yeast with excellent stress tolerance |
| topic | high protein yeast screening flavor stress tolerance |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20250121 |
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