Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors
IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at −607 and −137 with the level of IL-18...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2008/309721 |
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| author | Khripko Olga Pavlovna Sennikova Nadegda Sergeevna Lopatnikova Julia Anatolievna Khripko Jurii Ivanovich Filipenko Maxim Leonidovich Khrapov Evgenii Alexandrovich Gelfgat Evgenii L'vovich Yakushenko Elena Vladimirovna Kozlov Vladimir Alexandrovich Sennikov Sergei Vitalievich |
| author_facet | Khripko Olga Pavlovna Sennikova Nadegda Sergeevna Lopatnikova Julia Anatolievna Khripko Jurii Ivanovich Filipenko Maxim Leonidovich Khrapov Evgenii Alexandrovich Gelfgat Evgenii L'vovich Yakushenko Elena Vladimirovna Kozlov Vladimir Alexandrovich Sennikov Sergei Vitalievich |
| author_sort | Khripko Olga Pavlovna |
| collection | DOAJ |
| description | IL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at −607 and −137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs −607 and −137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia. |
| format | Article |
| id | doaj-art-085ba49b268f4f029535097910234236 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-085ba49b268f4f0295350979102342362025-02-03T05:46:46ZengWileyMediators of Inflammation0962-93511466-18612008-01-01200810.1155/2008/309721309721Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy DonorsKhripko Olga Pavlovna0Sennikova Nadegda Sergeevna1Lopatnikova Julia Anatolievna2Khripko Jurii Ivanovich3Filipenko Maxim Leonidovich4Khrapov Evgenii Alexandrovich5Gelfgat Evgenii L'vovich6Yakushenko Elena Vladimirovna7Kozlov Vladimir Alexandrovich8Sennikov Sergei Vitalievich9Laboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaLaboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaLaboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaPharmacogenomic Group, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, RussiaPharmacogenomic Group, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, RussiaPharmacogenomic Group, Institute of Chemical Biology and Fundamental Medicine, Novosibirsk 630090, RussiaLaboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaLaboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaLaboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaLaboratory of Molecular Immunology, Institute of Clinical Immunology, Siberian Branch of Russian Academy of Medical Sciences (SB RAMS), Novosibirsk 630099, RussiaIL-18 has proinflammatory effects and participates in both innate and adaptive cellular and humoral immunity. A number of SNPs that influence IL-18 production are found in the gene promoter region. We investigated the association of SNPs in the IL-18 promoter at −607 and −137 with the level of IL-18 protein production by PBMC from healthy donors from Southwestern Siberia. The genetic distribution of these SNPs in the promoter site was established by PCR. IL-18 protein production was determined by ELISA. Our results showed that PBMC from donors carrying allele 137C have lower levels of both spontaneous and LPS-stimulated IL-18 production. In contrast, PBMC from donors carrying allele 607A showed significant increases in spontaneous and stimulated IL-18 production compared to wild type. Our study suggests that the SNPs −607 and −137 in the promoter region of the IL-18 gene influence the level of IL-18 protein production by PBMC from healthy donors in Southwestern Siberia.http://dx.doi.org/10.1155/2008/309721 |
| spellingShingle | Khripko Olga Pavlovna Sennikova Nadegda Sergeevna Lopatnikova Julia Anatolievna Khripko Jurii Ivanovich Filipenko Maxim Leonidovich Khrapov Evgenii Alexandrovich Gelfgat Evgenii L'vovich Yakushenko Elena Vladimirovna Kozlov Vladimir Alexandrovich Sennikov Sergei Vitalievich Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors Mediators of Inflammation |
| title | Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors |
| title_full | Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors |
| title_fullStr | Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors |
| title_full_unstemmed | Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors |
| title_short | Association of Single Nucleotide Polymorphisms in the IL-18 Gene with Production of IL-18 Protein by Mononuclear Cells from Healthy Donors |
| title_sort | association of single nucleotide polymorphisms in the il 18 gene with production of il 18 protein by mononuclear cells from healthy donors |
| url | http://dx.doi.org/10.1155/2008/309721 |
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