Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro
Angiogenesis is an important event both in the development of allergic inflammatory responses and in the pathophysiology of tissue remodeling in allergic diseases. In the present study, therefore, we examined the influence of antihistamines on angiogenesis through the choice of epinastine hydrochlor...
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| Format: | Article |
| Language: | English |
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Wiley
2008-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2008/265095 |
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| author | K. Asano A. Furuta K. Kanai S. Sakaue H. Suzaki T. Hisamitsu |
| author_facet | K. Asano A. Furuta K. Kanai S. Sakaue H. Suzaki T. Hisamitsu |
| author_sort | K. Asano |
| collection | DOAJ |
| description | Angiogenesis is an important event both in the development of allergic inflammatory responses and in the pathophysiology of tissue remodeling in allergic diseases. In the present study, therefore, we examined the influence of antihistamines on angiogenesis through the choice of epinastine hydrochloride (EP) and murine mast cells in vitro. Mast cells (5×105 cells/mL) presensitized with murine IgE specific for ovalbumin (OVA) were stimulated with 10 ng/mL OVA in the presence of various concentrations of EP for 4 hours. The levels of angiogenesis factors, keratinocyte-derived chemokine (KC), tumor necrosis factor-α (TNF), and vascular endothelial growth factor (VEGF) in culture supernatants, were examined by ELISA. We also examined mRNA expression for the angiogenesis factors by RT-PCR. EP significantly inhibited the production of KC, TNF, and VEGF induced by IgE-dependent mechanism at more than 25 ng/mL. Semiquantitative analysis using RT-PCR showed that EP also significantly reduced mRNA expressions for KC, TNF, and VEGF. These results strongly suggest that EP suppresses angiogenesis factor production through the inhibition of mRNA expression in mast cells and results in favorable modification of clinical conditions of allergic diseases. |
| format | Article |
| id | doaj-art-084d8551c5054ab0a602767b2295278c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-084d8551c5054ab0a602767b2295278c2025-02-03T01:13:02ZengWileyMediators of Inflammation0962-93511466-18612008-01-01200810.1155/2008/265095265095Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In VitroK. Asano0A. Furuta1K. Kanai2S. Sakaue3H. Suzaki4T. Hisamitsu5Department of Physiology, School of NRS, Showa University, 1865 Toka-Ichiba, Midori-ku, Yokohama 226-8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, Tokyo, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, Tokyo, JapanDepartment of Physiology, School of NRS, Showa University, 1865 Toka-Ichiba, Midori-ku, Yokohama 226-8555, JapanDepartment of Otolaryngology, School of Medicine, Showa University, Shinagawa-ku, Tokyo, JapanDepartment of Physiology, School of NRS, Showa University, 1865 Toka-Ichiba, Midori-ku, Yokohama 226-8555, JapanAngiogenesis is an important event both in the development of allergic inflammatory responses and in the pathophysiology of tissue remodeling in allergic diseases. In the present study, therefore, we examined the influence of antihistamines on angiogenesis through the choice of epinastine hydrochloride (EP) and murine mast cells in vitro. Mast cells (5×105 cells/mL) presensitized with murine IgE specific for ovalbumin (OVA) were stimulated with 10 ng/mL OVA in the presence of various concentrations of EP for 4 hours. The levels of angiogenesis factors, keratinocyte-derived chemokine (KC), tumor necrosis factor-α (TNF), and vascular endothelial growth factor (VEGF) in culture supernatants, were examined by ELISA. We also examined mRNA expression for the angiogenesis factors by RT-PCR. EP significantly inhibited the production of KC, TNF, and VEGF induced by IgE-dependent mechanism at more than 25 ng/mL. Semiquantitative analysis using RT-PCR showed that EP also significantly reduced mRNA expressions for KC, TNF, and VEGF. These results strongly suggest that EP suppresses angiogenesis factor production through the inhibition of mRNA expression in mast cells and results in favorable modification of clinical conditions of allergic diseases.http://dx.doi.org/10.1155/2008/265095 |
| spellingShingle | K. Asano A. Furuta K. Kanai S. Sakaue H. Suzaki T. Hisamitsu Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro Mediators of Inflammation |
| title | Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro |
| title_full | Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro |
| title_fullStr | Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro |
| title_full_unstemmed | Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro |
| title_short | Inhibition of Angiogenic Factor Production from Murine Mast Cells by an Antiallergic Agent (Epinastine Hydrochloride) In Vitro |
| title_sort | inhibition of angiogenic factor production from murine mast cells by an antiallergic agent epinastine hydrochloride in vitro |
| url | http://dx.doi.org/10.1155/2008/265095 |
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