Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo
Peripheral nerve injury remains a clinical challenge with severe physiological and functional consequences. Despite the existence of multiple possible therapeutic approaches, until now, there is no consensus regarding the advantages of each option or the best methodology in promoting nerve regenerat...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2021/6613029 |
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| author | Rui D. Alvites Mariana V. Branquinho Ana C. Sousa Irina Amorim Rui Magalhães Filipa João Diogo Almeida Sandra Amado Justina Prada Isabel Pires Federica Zen Stefania Raimondo Ana L. Luís Stefano Geuna Artur S. P. Varejão Ana C. Maurício |
| author_facet | Rui D. Alvites Mariana V. Branquinho Ana C. Sousa Irina Amorim Rui Magalhães Filipa João Diogo Almeida Sandra Amado Justina Prada Isabel Pires Federica Zen Stefania Raimondo Ana L. Luís Stefano Geuna Artur S. P. Varejão Ana C. Maurício |
| author_sort | Rui D. Alvites |
| collection | DOAJ |
| description | Peripheral nerve injury remains a clinical challenge with severe physiological and functional consequences. Despite the existence of multiple possible therapeutic approaches, until now, there is no consensus regarding the advantages of each option or the best methodology in promoting nerve regeneration. Regenerative medicine is a promise to overcome this medical limitation, and in this work, chitosan nerve guide conduits and olfactory mucosa mesenchymal stem/stromal cells were applied in different therapeutic combinations to promote regeneration in sciatic nerves after neurotmesis injury. Over 20 weeks, the intervened animals were subjected to a regular functional assessment (determination of motor performance, nociception, and sciatic indexes), and after this period, they were evaluated kinematically and the sciatic nerves and cranial tibial muscles were evaluated stereologically and histomorphometrically, respectively. The results obtained allowed confirming the beneficial effects of using these therapeutic approaches. The use of chitosan NGCs and cells resulted in better motor performance, better sciatic indexes, and lower gait dysfunction after 20 weeks. The use of only NGGs demonstrated better nociceptive recoveries. The stereological evaluation of the sciatic nerve revealed identical values in the different parameters for all therapeutic groups. In the muscle histomorphometric evaluation, the groups treated with NGCs and cells showed results close to those of the group that received traditional sutures, the one with the best final values. The therapeutic combinations studied show promising outcomes and should be the target of new future works to overcome some irregularities found in the results and establish the combination of nerve guidance conduits and olfactory mucosa mesenchymal stem/stromal cells as viable options in the treatment of peripheral nerves after injury. |
| format | Article |
| id | doaj-art-082fd402c4d748238da4e6f38c5cac54 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-082fd402c4d748238da4e6f38c5cac542025-02-03T01:25:26ZengWileyStem Cells International1687-966X1687-96782021-01-01202110.1155/2021/66130296613029Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In VivoRui D. Alvites0Mariana V. Branquinho1Ana C. Sousa2Irina Amorim3Rui Magalhães4Filipa João5Diogo Almeida6Sandra Amado7Justina Prada8Isabel Pires9Federica Zen10Stefania Raimondo11Ana L. Luís12Stefano Geuna13Artur S. P. Varejão14Ana C. Maurício15Departamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalDepartamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalDepartamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalDepartamento de Patologia e Imunologia Molecular, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalUniversidade Católica Portuguesa, Centro de Biotecnologia e Química Fina (CBQF)-Laboratório Associado, Escola Superior de Biotecnologia, Rua Diogo Botelho 1327, 4169-005 Porto, PortugalCentro Interdisciplinar de Estudo da Performance Humana (CIPER), Faculdade de Motricidade Humana (FMH), Universidade de Lisboa (ULisboa), 1495-751 Cruz Quebrada, PortugalInstituto Politécnico de Leiria, Unidade de Investigação em Saúde da Escola Superior de Saúde de Leiria (UIS-IPL), PortugalInstituto Politécnico de Leiria, Unidade de Investigação em Saúde da Escola Superior de Saúde de Leiria (UIS-IPL), PortugalCentro de Ciência Animal e Veterinária (CECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, PortugalCentro de Ciência Animal e Veterinária (CECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, PortugalDepartment of Clinical and Biological Sciences, Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalyDepartment of Clinical and Biological Sciences, Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalyDepartamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalDepartment of Clinical and Biological Sciences, Neuroscience Institute Cavalieri Ottolenghi, University of Turin, Regione Gonzole 10, 10043 Orbassano, ItalyCentro de Ciência Animal e Veterinária (CECAV), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, PortugalDepartamento de Clínicas Veterinárias, Instituto de Ciências Biomédicas de Abel Salazar (ICBAS), Universidade do Porto (UP), Rua de Jorge Viterbo Ferreira, No. 228, 4050-313 Porto, PortugalPeripheral nerve injury remains a clinical challenge with severe physiological and functional consequences. Despite the existence of multiple possible therapeutic approaches, until now, there is no consensus regarding the advantages of each option or the best methodology in promoting nerve regeneration. Regenerative medicine is a promise to overcome this medical limitation, and in this work, chitosan nerve guide conduits and olfactory mucosa mesenchymal stem/stromal cells were applied in different therapeutic combinations to promote regeneration in sciatic nerves after neurotmesis injury. Over 20 weeks, the intervened animals were subjected to a regular functional assessment (determination of motor performance, nociception, and sciatic indexes), and after this period, they were evaluated kinematically and the sciatic nerves and cranial tibial muscles were evaluated stereologically and histomorphometrically, respectively. The results obtained allowed confirming the beneficial effects of using these therapeutic approaches. The use of chitosan NGCs and cells resulted in better motor performance, better sciatic indexes, and lower gait dysfunction after 20 weeks. The use of only NGGs demonstrated better nociceptive recoveries. The stereological evaluation of the sciatic nerve revealed identical values in the different parameters for all therapeutic groups. In the muscle histomorphometric evaluation, the groups treated with NGCs and cells showed results close to those of the group that received traditional sutures, the one with the best final values. The therapeutic combinations studied show promising outcomes and should be the target of new future works to overcome some irregularities found in the results and establish the combination of nerve guidance conduits and olfactory mucosa mesenchymal stem/stromal cells as viable options in the treatment of peripheral nerves after injury.http://dx.doi.org/10.1155/2021/6613029 |
| spellingShingle | Rui D. Alvites Mariana V. Branquinho Ana C. Sousa Irina Amorim Rui Magalhães Filipa João Diogo Almeida Sandra Amado Justina Prada Isabel Pires Federica Zen Stefania Raimondo Ana L. Luís Stefano Geuna Artur S. P. Varejão Ana C. Maurício Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo Stem Cells International |
| title | Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo |
| title_full | Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo |
| title_fullStr | Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo |
| title_full_unstemmed | Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo |
| title_short | Combined Use of Chitosan and Olfactory Mucosa Mesenchymal Stem/Stromal Cells to Promote Peripheral Nerve Regeneration In Vivo |
| title_sort | combined use of chitosan and olfactory mucosa mesenchymal stem stromal cells to promote peripheral nerve regeneration in vivo |
| url | http://dx.doi.org/10.1155/2021/6613029 |
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