Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice
The study aimed to investigate the effects of differentiated exercise regimes on high fat-induced metabolic and inflammatory pathways. Mice were fed a standard diet (ST) or a high fat diet (HFD) and subjected to regular endurance training (ET) or resistance training (RT). After 10 weeks body weight,...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/4536470 |
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| author | Cornelia Mardare Karsten Krüger Gerhard Liebisch Michael Seimetz Aline Couturier Robert Ringseis Jochen Wilhelm Norbert Weissmann Klaus Eder Frank-Christoph Mooren |
| author_facet | Cornelia Mardare Karsten Krüger Gerhard Liebisch Michael Seimetz Aline Couturier Robert Ringseis Jochen Wilhelm Norbert Weissmann Klaus Eder Frank-Christoph Mooren |
| author_sort | Cornelia Mardare |
| collection | DOAJ |
| description | The study aimed to investigate the effects of differentiated exercise regimes on high fat-induced metabolic and inflammatory pathways. Mice were fed a standard diet (ST) or a high fat diet (HFD) and subjected to regular endurance training (ET) or resistance training (RT). After 10 weeks body weight, glucose tolerance, fatty acids (FAs), circulating ceramides, cytokines, and immunological mediators were determined. The HFD induced a significant increase in body weight and a disturbed glucose tolerance (p<0.05). An increase of plasma FA, ceramides, and inflammatory mediators in adipose tissue and serum was found (p<0.05). Both endurance and resistance training decreased body weight (p<0.05) and reduced serum ceramides (p<0.005). While RT attenuated the increase of NLRP-3 (RT) expression in adipose tissue, ET was effective in reducing TNF-α and IL-18 expression. Furthermore, ET reduced levels of MIP-1γ, while RT decreased levels of IL-18, MIP-1γ, Timp-1, and CD40 in serum (p<0.001), respectively. Although both exercise regimes improved glucose tolerance (p<0.001), ET was more effective than RT. These results suggest that exercise improves HFD-induced complications possibly through a reduction of ceramides, the reduction of inflammasome activation in adipose tissues, and a systemic downregulation of inflammatory cytokines. |
| format | Article |
| id | doaj-art-081879737e03492586ce1a65ab5faba4 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-081879737e03492586ce1a65ab5faba42025-02-03T05:57:09ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/45364704536470Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in MiceCornelia Mardare0Karsten Krüger1Gerhard Liebisch2Michael Seimetz3Aline Couturier4Robert Ringseis5Jochen Wilhelm6Norbert Weissmann7Klaus Eder8Frank-Christoph Mooren9Department of Sports Medicine, Institute of Sports Sciences, Justus-Liebig-University, 35394 Giessen, GermanyDepartment of Sports Medicine, Institute of Sports Sciences, Justus-Liebig-University, 35394 Giessen, GermanyInstitute of Clinical Chemistry and Laboratory Medicine, University of Regensburg, 93053 Regensburg, GermanyUniversities of Giessen and Marburg Lung Center, German Center for Lung Research, Excellence Cluster Cardio-Pulmonary System, Justus-Liebig-University, 35392 Giessen, GermanyInstitute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University, 35392 Giessen, GermanyInstitute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University, 35392 Giessen, GermanyUniversities of Giessen and Marburg Lung Center, German Center for Lung Research, Excellence Cluster Cardio-Pulmonary System, Justus-Liebig-University, 35392 Giessen, GermanyUniversities of Giessen and Marburg Lung Center, German Center for Lung Research, Excellence Cluster Cardio-Pulmonary System, Justus-Liebig-University, 35392 Giessen, GermanyInstitute of Animal Nutrition and Nutrition Physiology, Justus-Liebig-University, 35392 Giessen, GermanyDepartment of Sports Medicine, Institute of Sports Sciences, Justus-Liebig-University, 35394 Giessen, GermanyThe study aimed to investigate the effects of differentiated exercise regimes on high fat-induced metabolic and inflammatory pathways. Mice were fed a standard diet (ST) or a high fat diet (HFD) and subjected to regular endurance training (ET) or resistance training (RT). After 10 weeks body weight, glucose tolerance, fatty acids (FAs), circulating ceramides, cytokines, and immunological mediators were determined. The HFD induced a significant increase in body weight and a disturbed glucose tolerance (p<0.05). An increase of plasma FA, ceramides, and inflammatory mediators in adipose tissue and serum was found (p<0.05). Both endurance and resistance training decreased body weight (p<0.05) and reduced serum ceramides (p<0.005). While RT attenuated the increase of NLRP-3 (RT) expression in adipose tissue, ET was effective in reducing TNF-α and IL-18 expression. Furthermore, ET reduced levels of MIP-1γ, while RT decreased levels of IL-18, MIP-1γ, Timp-1, and CD40 in serum (p<0.001), respectively. Although both exercise regimes improved glucose tolerance (p<0.001), ET was more effective than RT. These results suggest that exercise improves HFD-induced complications possibly through a reduction of ceramides, the reduction of inflammasome activation in adipose tissues, and a systemic downregulation of inflammatory cytokines.http://dx.doi.org/10.1155/2016/4536470 |
| spellingShingle | Cornelia Mardare Karsten Krüger Gerhard Liebisch Michael Seimetz Aline Couturier Robert Ringseis Jochen Wilhelm Norbert Weissmann Klaus Eder Frank-Christoph Mooren Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice Journal of Diabetes Research |
| title | Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice |
| title_full | Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice |
| title_fullStr | Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice |
| title_full_unstemmed | Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice |
| title_short | Endurance and Resistance Training Affect High Fat Diet-Induced Increase of Ceramides, Inflammasome Expression, and Systemic Inflammation in Mice |
| title_sort | endurance and resistance training affect high fat diet induced increase of ceramides inflammasome expression and systemic inflammation in mice |
| url | http://dx.doi.org/10.1155/2016/4536470 |
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