Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents
Deep eutectic solvents (DES), as a new, efficient, and green solvents, have attracted more attention in recent years. This study was performed to extract Polygonatum sibiricum polysaccharides using DES (DPSP). Subsequently, DPSP was subjected to DEAE-Sepharose Fast Flow and Sephadex G-100 column for...
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2023/3147644 |
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| author | Jikun Meng Chunbo Guan Qiufeng Chen Xiao Pang Heqin Wang Xinwen Cui Ran Ye Xiuqing Zhang |
| author_facet | Jikun Meng Chunbo Guan Qiufeng Chen Xiao Pang Heqin Wang Xinwen Cui Ran Ye Xiuqing Zhang |
| author_sort | Jikun Meng |
| collection | DOAJ |
| description | Deep eutectic solvents (DES), as a new, efficient, and green solvents, have attracted more attention in recent years. This study was performed to extract Polygonatum sibiricum polysaccharides using DES (DPSP). Subsequently, DPSP was subjected to DEAE-Sepharose Fast Flow and Sephadex G-100 column for further purification (DPSP-3). Structural characterization and immunomodulatory activity in RAW264.7 cells were performed. Results showed that the yield of DPSP was (15.62 ± 0.71)%, and its yield was 1.53 times that of the polysaccharides extracted using the pharmacopoeia method (PSP). DPSP-3 had a smaller molecular weight (3.2 × 106 Da) and a higher ratio of galactose (65.75%) and mannose (19.76%) as compared to PSP. The scanning electron microscope (SEM) showed that DPSP-3 had curly shape, lamellar surface, and a few spherical edges, which were significantly different from PSP and DPSP. Both PSP and DPSP-3 could significantly improve phagotrophic capacity and increase the release of ROS, NO, IL-6, and TNF-α in RAW 264.7 cells, and DPSP-3 had better immunomodulatory activity. In summary, compared to the pharmacopoeia method, polysaccharides prepared using the DES extraction method showed higher yield and improved immunomodulatory activity. |
| format | Article |
| id | doaj-art-0810b6f507e04d9da1e950ffadd228e3 |
| institution | Kabale University |
| issn | 1745-4557 |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-0810b6f507e04d9da1e950ffadd228e32025-02-03T05:48:40ZengWileyJournal of Food Quality1745-45572023-01-01202310.1155/2023/3147644Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic SolventsJikun Meng0Chunbo Guan1Qiufeng Chen2Xiao Pang3Heqin Wang4Xinwen Cui5Ran Ye6Xiuqing Zhang7College of Food Science and Nutritional EngineeringCollege of Food Science and Nutritional EngineeringCollege of Food Science and Nutritional EngineeringCollege of Food Science and Nutritional EngineeringCollege of Food Science and Nutritional EngineeringCollege of Food Science and Nutritional EngineeringBeijing Yuanmai Shanqiu Food Co.College of Food Science and Nutritional EngineeringDeep eutectic solvents (DES), as a new, efficient, and green solvents, have attracted more attention in recent years. This study was performed to extract Polygonatum sibiricum polysaccharides using DES (DPSP). Subsequently, DPSP was subjected to DEAE-Sepharose Fast Flow and Sephadex G-100 column for further purification (DPSP-3). Structural characterization and immunomodulatory activity in RAW264.7 cells were performed. Results showed that the yield of DPSP was (15.62 ± 0.71)%, and its yield was 1.53 times that of the polysaccharides extracted using the pharmacopoeia method (PSP). DPSP-3 had a smaller molecular weight (3.2 × 106 Da) and a higher ratio of galactose (65.75%) and mannose (19.76%) as compared to PSP. The scanning electron microscope (SEM) showed that DPSP-3 had curly shape, lamellar surface, and a few spherical edges, which were significantly different from PSP and DPSP. Both PSP and DPSP-3 could significantly improve phagotrophic capacity and increase the release of ROS, NO, IL-6, and TNF-α in RAW 264.7 cells, and DPSP-3 had better immunomodulatory activity. In summary, compared to the pharmacopoeia method, polysaccharides prepared using the DES extraction method showed higher yield and improved immunomodulatory activity.http://dx.doi.org/10.1155/2023/3147644 |
| spellingShingle | Jikun Meng Chunbo Guan Qiufeng Chen Xiao Pang Heqin Wang Xinwen Cui Ran Ye Xiuqing Zhang Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents Journal of Food Quality |
| title | Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents |
| title_full | Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents |
| title_fullStr | Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents |
| title_full_unstemmed | Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents |
| title_short | Structural Characterization and Immunomodulatory Activity of Polysaccharides from Polygonatum sibiricum Prepared with Deep Eutectic Solvents |
| title_sort | structural characterization and immunomodulatory activity of polysaccharides from polygonatum sibiricum prepared with deep eutectic solvents |
| url | http://dx.doi.org/10.1155/2023/3147644 |
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