Development and validation of a RP- HPLC method for the quantitation studies of bromadiolone in Ratitox F
An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of bromadiolone (hydroxycoumarins) in Ratitox F product – rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 Hypersil C18 (250 mm x 4.6 mm i.e.; 5 ìm...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Asociaţia Naţională a Fabricanţilor de Produse de Uz Veterinar
2011-12-01
|
| Series: | Medicamentul Veterinar |
| Subjects: | |
| Online Access: | http://www.veterinarypharmacon.com/docs/999-2011-ART.%206.%20eng.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | An isocratic high-performance liquid chromatography (HPLC) procedure was developed for the quantitative determination of bromadiolone (hydroxycoumarins) in Ratitox F product – rodenticide. HPLC separation was carried out by reversed phase chromatography ODS 2 Hypersil C18 (250 mm x 4.6 mm i.e.; 5 ìm particle size), held in thermostat at 25°C. The mobile phase consisted of methanol/0.1% aqueous solution phosphoric acid (90/10v/v), with a flow rate of 1 ml/min and with UV detection at 265 nm. In order to validate the method, the following parameters have been investigated- linearity (r2 = 0.9999), range, precision, accuracy, specificity, limit of detection and limit of quantification. The described method can be successfully applied for the analysis of Ratitox F – rodenticide. |
|---|---|
| ISSN: | 1843-9527 2069-2463 |